Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China)

Xü, Wei; Lin, Wei-Ming; Wang, Zhichao; Gao, Yuelin; Luo, Yu; Grossart, Hans‐Peter; Guo, Ying; Gao, Qiancheng; Huang, Lixing; Luo, Zengwei

doi:10.1016/j.csbj.2021.07.040

Cited by 12 publications

(10 citation statements)

References 56 publications

(102 reference statements)

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“…Total RNA was extracted using TRIzol reagent (Invitrogen, USA) following the manufacturer's instructions ( 11 , 50 ). Reverse transcription was performed from 2.0 mg of total RNA using A Reverse Mu-MLV cDNA Synthesis Kit (TransGen Biotech Co., Ltd., Beijing, China) following the manufacturer's instructions ( 11 , 51 ).…”

Section: Methodsmentioning

confidence: 99%

Virulence regulation of Zn2+ uptake system znuABC on mesophilic Aeromonas salmonicida SRW-OG1

et al. 2023

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Psychrophilic Aeromonas salmonicida could not grow above 25°C and therefore thought unable to infect mammals and humans. In our previous study, a mesophilic A. salmonicida SRW-OG1 was isolated from Epinephelus coioides with furunculosis. Through the analysis of preliminary RNA-seq, it was found that the Zn2+ uptake related genes znuA, znuB and znuC might be involved in the virulence regulation of A. salmonicida SRW-OG1. Therefore, the purpose of this study was to explore the effect of znuABC silencing on the virulence regulation of A. salmonicida SRW-OG1. The results showed that the growth of the znuA-RNAi, znuB-RNAi, and znuC-RNAi strains was severely restricted under the Fe2+ starvation, but surprisingly there was no significant difference under the Zn2+ restriction. In the absence of Zn2+ and Fe2+, the expression level of znuABC was significantly increased. The motility, biofilm formation, adhesion and hemolysis of the znuA-RNAi, znuB-RNAi, and znuC-RNAi strains were significantly reduced. We also detected the expression of znuABC under different growth periods, temperatures, pH, as well as Cu2+ and Pb2+ stresses. The results showed that znuABC was significantly up-regulated in the logarithmic phase and the decline phase of A. salmonicida. Interestingly, the trend of expression levels of the znuABC at 18, 28, and 37°C was reversed to another Zn2+ uptake related gene zupT. Taken together, these indicated that the znuABC was necessary for A. salmonicida SRW-OG1 pathogenicity and environmental adaptability, and was cross regulated by iron starvation, but it was not irreplaceable for A. salmonicida SRW-OG1 Zn2+ uptake in the host.

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Section: Methodsmentioning

confidence: 99%

Virulence regulation of Zn2+ uptake system znuABC on mesophilic Aeromonas salmonicida SRW-OG1

et al. 2023

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“…It was cultured in Luria–Bertani (LB) medium (Sangon Biotech, Shanghai, China) at 18, 28 or 37 °C (220 rpm). For RNA extraction, bacterial cultures were adjusted to OD 560 = 0.3 [ 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

“…Hemolytic capacity of A . salmonicida was detected according to the methods described by Wei [ 25 ]. We centrifuged 100 μL of fresh sheep blood (Ping Rui Biotechnology Co., Ltd., Beijing, China) at 3000 rpm for 10 min at 25 °C, and the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Hemolytic Activity of Mesophilic Aeromonas salmonicida SRW-OG1 Is Brought about by Elevated Temperatures

et al. 2022

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Aeromonas salmonicida is a well-known cold-water pathogenic bacterium. Previously, we reported the first isolation of pathogenic A. salmonicida from diseased Epinephelus coioides, a kind of warm-water fish, and it was proved to be a putative mesophilic strain with potent pathogenicity to humans. In order to investigate the mechanisms underlying mesophilic growth ability and virulence, the transcriptome of A. salmonicida SRW-OG1 at 18, 28, and 37 °C was analyzed. The transcriptome of A. salmonicida SRW-OG1 at different temperatures showed a clear separation boundary, which might provide valuable information for the temperature adaptation and virulence regulation of A. salmonicida SRW-OG1. Interestingly, aerA and hlyA, the hemolytic genes encoding aerolysin and hemolysin, were found to be significantly up-regulated at 28 and 37 °C. Since aerolysin and hemolysin are the most well-known and -characterized virulence factors of pathogenic Aeromonas strains, the induction of aerA and hlyA was associated with the mesophilic virulence. Further study proved that the extracellular products (ECPs) purchased from A. salmonicida SRW-OG1 cultured at 28 and 37 °C showed elevated hemolytic activity and virulence than those at 18 °C. Moreover, the silence of aerA and hlyA led to significantly decreased hemolysis and virulence. Taken together, our results revealed that the mesophilic virulence of A. salmonicida SRW-OG1 might be due to the enhanced expression of aerA and hlyA induced by elevated temperatures.

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“…The hemolysis assay for P. plecoglossicida was carried out as described by Wei et al. ( Tsou and Zhu, 2010 ; Xu et al., 2021 ). 100 μl of fresh sheep blood (Ping Rui Biotechnology Co., Ltd., Beijing, China) was centrifuged at 2,500 rpm for 5 min, and the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

“…TRIzol reagent (Invitrogen, Carlsbad, CA, USA) was used for total RNA extraction from bacterial cells, as directed by the manufacturer. Reverse transcription was carried out with A Reverse Aid Mu-MLV cDNA synthesis kit (TransGen Biotech Co., Ltd., Beijing, China) from 2.0 mg total RNA, as instructed by the manufacturer ( Ge et al., 2020 ; Huang et al., 2020a ; Xu et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

Wang

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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Pseudomonas plecoglossicida is an aerobic Gram-negative bacterium, which is the pathogen of “Visceral white spot disease” in large yellow croaker. P. plecoglossicida is a temperature-dependent bacterial pathogen in fish, which not only reduces the yield of large yellow croaker but also causes continuous transmission of the disease, seriously endangering the healthy development of fisheries. In this study, a mutant strain of fusA was constructed using homologous recombination technology. The results showed that knockout of P. plecoglossicida fusA significantly affected the ability of growth, adhesion, and biofilm formation. Temperature, pH, H2O2, heavy metals, and the iron-chelating agent were used to treat the wild type of P. plecoglossicida; the results showed that the expression of fusA was significantly reduced at 4°C, 12°C, and 37°C. The expression of fusA was significantly increased at pH 4 and 5. Cu2+ has a significant inducing effect on the expression of fusA, but Pb2+ has no obvious effect; the expression of fusA was significantly upregulated under different concentrations of H2O2. The expression of the fusA gene was significantly upregulated in the 0.5~4-μmol/l iron-chelating agent. The expression level of the fusA gene was significantly upregulated after the logarithmic phase. It was suggested that fusA included in the TBDR family not only was involved in the transport of ferredoxin but also played important roles in the pathogenicity and environment adaptation of P. plecoglossicida.

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Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China)

Abstract: Graphical abstract

Cited by 12 publications

References 56 publications

Virulence regulation of Zn2+ uptake system znuABC on mesophilic Aeromonas salmonicida SRW-OG1

Virulence regulation of Zn2+ uptake system znuABC on mesophilic Aeromonas salmonicida SRW-OG1

Enhanced Hemolytic Activity of Mesophilic Aeromonas salmonicida SRW-OG1 Is Brought about by Elevated Temperatures

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

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