Disentangling the Impact of Sulfur Limitation on Exopolysaccharide and Functionality of Alr2882 by In Silico Approaches in Anabaena sp. PCC 7120

Kharwar, Surbhi; Bhattacharjee, Samujjal; Mishra, Arun Kumar

doi:10.1007/s12010-021-03501-3

Cited by 15 publications

(4 citation statements)

References 87 publications

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“…Microalgae are considered to be the most efficient organisms in converting solar energy. Additionally, microalgae do not require cultivated land, so they do not compete with food crops on arable land [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

et al. 2021

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Our study focused on investigating the possibilities of controlling the accumulation of carbohydrates in certain microalgae species (Arthrospira platensis Gomont, Chlorella vulgaris Beijer, and Dunaliella salina Teod) to determine their potential in biofuel production (biohydrogen). It was found that after the introduction of carbohydrates (0.05 g⋅L−1) into the nutrient medium, the growth rate of the microalgae biomass increased, and the accumulation of carbohydrates reached 41.1%, 47.9%, and 31.7% for Arthrospira platensis, Chlorella vulgaris, and Dunaliella salina, respectively. Chlorella vulgaris had the highest total carbohydrate content (a mixture of glucose, fructose, sucrose, and maltose, 16.97%) among the studied microalgae, while for Arthrospira platensis and Dunaliella salina, the accumulation of total carbohydrates was 9.59% and 8.68%, respectively. Thus, the introduction of carbohydrates into the nutrient medium can stimulate their accumulation in the microalgae biomass, an application of biofuel production (biohydrogen).

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Section: Introductionmentioning

confidence: 99%

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

et al. 2021

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“…The distribution of putative OCAs among cyanobacteria exhibited a clear relation with larger genome size and morphological complexities (Kharwar et al, 2021), since the Oscillatoriales and Nostocales abundantly harbored them. This assumption was also supported by the absence of OCAs in the majority of the unicellular members of Synechococcales and their presence in the filamentous ones.…”

Section: Rich Pool Of Cyanobacterial Ocas Having Considerable Diversity and Sharing A Complex Phylogenetic Relationshipmentioning

confidence: 99%

Insights Into the Phylogenetic Distribution, Diversity, Structural Attributes, and Substrate Specificity of Putative Cyanobacterial Orthocaspases

2021

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The functionality of caspase homologs in prokaryotic cell execution has been perceived, yet the dimensions of their metabolic pertinence are still cryptic. Here, a detailed in silico study on putative cyanobacterial caspase homologs, termed orthocaspases, in a sequenced genome of 132 strains was performed. We observed that 473 putative orthocaspases were distributed among 62% cyanobacterial strains subsumed within all the taxonomical orders. However, high diversity among these orthocaspases was also evident as the conventional histidine–cysteine (HC) dyad was present only in 72.03% of orthocaspases (wild-type), whereas the rest 28.18% were pseudo-variants having substituted the catalytic dyad. Besides, the presence of various accessory functional domains with Peptidase C14 probably suggested the multifunctionality of the orthocaspases. Moreover, the early origin and emergence of wild-type orthocaspases were conferred by their presence in Gloeobacter; however, the complex phylogeny displayed by these caspase-homologs perhaps suggested horizontal a gene transfer for their acquisition. However, morpho-physiological advancements and larger genome size favored the acquisition of orthocaspases. Moreover, the conserved caspase hemoglobinase fold not only in the wild-type but also in the pseudo-orthocaspases in Nostoc sp. PCC 7120 ascertained the least effect of catalytic motifs in the protein tertiary structure. Further, the 100-ns molecular dynamic simulation and molecular mechanics/generalized born surface area exhibited stable binding of arginylarginine dipeptide with wild-type orthocaspase of Nostoc sp. PCC 7120, displaying arginine-P1 specificity of wild-type orthocaspases. This study deciphered the distribution, diversity, domain architecture, structure, and basic substrate specificity of putative cyanobacterial orthocaspases, which may aid in functional investigations in the future.

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“…They have relatively high photosynthetic efficiency and growth rates, and some species grow well in brackish or seawater, as well as in wastewater of various origins. Microalgae are known for their potential to produce bioproducts and are promising targets for biotechnology due to their lipid, carbohydrate, protein content and photosynthetic activity [1,2].…”

Section: Introductionmentioning

confidence: 99%

The influence of varying concentrations of CO₂ on the buildup of carbohydrates in microalgae biomass

Mahmoudi,

Kossalbayev,

Sarsekeyeva

et al. 2024

BIO Web Conf.

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Microalgae have gained popularity over the century due to their numerous intrinsic properties superior to higher plants, making them a potential target and feedstock for the development of biotechnological products in various fields. The storage of carbohydrates within microalgae cells positions them as a promising reservoir for biofuel production and a range of other valuable bioproducts, encompassing biological compounds, nutritional supplements, and more. Key determinants influencing microalgae carbohydrate levels comprise nutrient availability, light intensity, temperature, and CO2 concentrations. In this context, the CO2 concentration plays a key role, as it is one of the main factors influencing the photosynthetic processes. This study aimed to explore the impact of different CO2 concentrations on the carbohydrate profile of biomass sourced from Chlorella vulgaris sp. BB-2 and Scenedesmus quadicauda B-1. The findings revealed that a CO2 concentration of 2% v/v notably enhanced carbohydrate accumulation, reaching 75.5% for Chlorella vulgaris sp. BB-2 and 72.0% for Scenedesmus quadicauda B-1.

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Disentangling the Impact of Sulfur Limitation on Exopolysaccharide and Functionality of Alr2882 by In Silico Approaches in Anabaena sp. PCC 7120

Cited by 15 publications

References 87 publications

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

Insights Into the Phylogenetic Distribution, Diversity, Structural Attributes, and Substrate Specificity of Putative Cyanobacterial Orthocaspases

The influence of varying concentrations of CO₂ on the buildup of carbohydrates in microalgae biomass

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Disentangling the Impact of Sulfur Limitation on Exopolysaccharide and Functionality of Alr2882 by In Silico Approaches in Anabaena sp. PCC 7120

Cited by 15 publications

References 87 publications

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

Insights Into the Phylogenetic Distribution, Diversity, Structural Attributes, and Substrate Specificity of Putative Cyanobacterial Orthocaspases

The influence of varying concentrations of CO2 on the buildup of carbohydrates in microalgae biomass

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The influence of varying concentrations of CO₂ on the buildup of carbohydrates in microalgae biomass