Disinfection efficacy of photon-induced photoacoustic streaming on root canals infected with Enterococcus faecalis

Olivi, Giovanni; DiVito, Enrico; Peters, Ove A.; Kaitsas, Vassilios; Angiero, Francesca; Signore, Antonio; Benedicenti, Stefano

doi:10.14219/jada.2014.46

Cited by 46 publications

(34 citation statements)

References 28 publications

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“…A standard curve of E. faecalis was performed using the assay with a known number of bacteria. Similar results showing improved disinfection capability of PIPS irrigation have been reported using different analytical methodologies [26][27][28]. The challenges of determining antibacterial efficacy are compounded by the difficulty in assessing what is actually being measured.…”

supporting

confidence: 75%

“…Techniques which are commonly used, such as paper point sampling followed by CFU counting can determine general bacterial counting from the coronal third which can exclude information from the apical third, where disinfection is more indicative of irrigation efficiency and more difficult to access. Measurement of the apical third cleanliness has been attempted by direct-access methods, as well as split-model methods, both requiring structural modification of the dentin and apical complexities [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. While the method used in this experiment contains similar shortcomings to the paper point/CFU method with regards to sampling location, it provides expeditious sample collection, reuse of samples in order for the same sample to serve as a control, and a well-known, reliable methodology for bacterial quantification.…”

mentioning

confidence: 99%

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In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

Babu¹

2017

JDHODT

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Objective: This in vitro study was designed to compare the disinfection potential of an Er:YAG photon-initiated photoacousitc (PIPS) irrigation mechanism to standard needle irrigation (SNI) in eliminating biofilms from complex root canal systems. Method and materials:Twenty-four extracted mandibular molar teeth exhibiting a Vertucci Type II canal system were selected and underwent canal instrumentation to create either a minimal (20/.04) or fully-flared (25/.06) preparation. Teeth were inoculated with bacterial plaque and incubated for 3 weeks creating a mature biofilm. Both instrumentation groups were sub-divided according to mode of canal disinfection: sterile saline or sodium hypochlorite (NaOCl) with PIPS laser-activation or same irrigants delivered with SNI. Each irrigation protocol used similar quantities of NaOCl or saline over the same time periods. Viable intracanal bacteria were quantified by MTT bacterial viability assay. Sub-groups were analyzed for statistical significance by ANOVA, followed by Scheffe's F-test (P = .05).Results: All saline groups showed significantly more viable bacteria after irrigation than the corresponding NaOCl sub-groups. The #20/.04 sub-group that underwent PIPS and NaOCl irrigation had greater disinfection than the SNI counterpart, which was not significant (P > 0.05). The #25/.06 PIPS/NaOCl subgroup showed greater disinfection than SNI (P > 0.0008). All other groups showed no significant differences in disinfection of root canals. Conclusion:In this in vitro study PIPS laser-activated irrigation achieved higher disinfection in #25/.06 fully-flared preparations than SNI. Although not significantly different, greater disinfection was found in the #20/.04 minimal canal preparations that were treated with PIPS irrigation compared to SNI. Keywords: In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars 2/5Copyright: ©2017 Garcia-Godoy et al. [19,20]. The combination of varying tip size and taper continues to be studied, aimed at improving canal disinfection while conserving dentin. As apical preparation size increases, irrigant replacement was enhanced, providing space existed between the needle and the canal wall [21]. Increased canal taper also improved fluid interchange and reduced the risk of extrusion; minimal taper with large apical preparations also improved irrigant replacement [22]. Ex vivo investigation of the optimal tip size taper combination for laser activated irrigation have yet to be determined. The objective of this study was to examine the disinfection of intentionally contaminated Type II canals in the mesial root of mandibular molars by comparing needle flushing versus PIPS activation during endodontic irrigation of complex Vertucci Type II canal systems of two different sizes and taper. The null hypothesis was that no difference exists between bacterial disinfection present in #20/.04 taper and #25/.06 flared taper preparations using either PIPS or needle irrigation. Methods and MaterialsToot...

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supporting

confidence: 75%

mentioning

confidence: 99%

In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

Babu¹

2017

JDHODT

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“…PIPS uses a sub-ablative photoacoustic technique that allows the penetration of the irrigating solution into inaccessible areas of the root canal system, killing bacteria not by a thermal event but rather by antimicrobial irrigants that are activated by the photomechanical effects of the PIPS-tapered and stripped laser tip (Jaramillo et al 2012). Previous studies have shown that PIPS in combination with 5.25 and 6 % sodium hypochlorite is an effective means of eliminating resistant bacteria such as E. faecalis from the root canal system (Jaramillo et al 2012;Olivi et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Root canal disinfection comparing conventional irrigation vs photon-induced photoacoustic streaming (PIPS) using a buffered 0.5 % sodium hypochlorite solution

et al. 2016

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Background: The purpose of this study was to assess the antimicrobial effect of a buffered 0.5 % sodium hypochlorite solution activated by photon-induced photoacoustic streaming compared to conventional irrigation. Methods: The canals on 48 single canal lower bicuspids were cleaned and shaped using rotary instrumentation. All roots were autoclaved for 20 min. Thirty-six of the roots were placed in glass flasks with blood heart infusion media and Enterococcus faecalis (ATCC 4082) for 4 weeks. The remaining 12 roots were placed in a sterile environment and served as negative controls. The contaminated roots were irrigated by conventional means using a buffered 0.5 % sodium hypochlorite solution with or without photon-induced photoacoustic streaming (PIPS) activation (n = 12 each group). The remaining 12 roots did not receive any treatment and served as positive controls. The apical 3 mm of each tooth was sectioned and pulverized. The pulverized samples were collected and placed in Eppendorf micro-centrifuge tubes with sterile phosphate-buffered saline. Thirty MicroLiters of the collected samples was then placed in the blood heart infusion media and incubated for 24 h at 37°C. Colony forming units (CFU) were compared with Wilcoxon signed ranked test. Mann-Whitney U test was used to assess PIPS effectiveness in comparison with conventional irrigation. Results: Both regimens reduced significantly the number of CFU; however, reduction was significantly higher for the PIPS group (p = 0.002). Conclusion: Buffered 0.5 % sodium hypochlorite delivered by conventional method was effective in removing E. faecalis from contaminated root canals; however, activation of a buffered 0.5 % sodium hypochlorite solution by PIPS significantly increased its antimicrobial capacity.

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“…27 However, they did not evaluate the changes in root dentin roughness caused by LAI. An increase in roughness contributes to removal of the dentin with endodontics files, and promotes micromechanical interlocking of root sealers to dentin.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nd:YAG (1064-nm) and Diode Laser (980-nm) EDTA Agitation on Root Dentin Ultrastructure Properties

Macedo

Colucci

Messias

et al. 2015

Photomedicine and Laser Surgery

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Disinfection efficacy of photon-induced photoacoustic streaming on root canals infected with Enterococcus faecalis

Cited by 46 publications

References 28 publications

In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

In-Vitro Evaluation of the Efficacy of PIPS Irrigation System on Disinfection of Type 2 Canal Systems in Molars

Root canal disinfection comparing conventional irrigation vs photon-induced photoacoustic streaming (PIPS) using a buffered 0.5 % sodium hypochlorite solution

Effect of Nd:YAG (1064-nm) and Diode Laser (980-nm) EDTA Agitation on Root Dentin Ultrastructure Properties

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