Disinfection of Aerosolized Pathogenic Fungi on Laboratory Surfaces

Kruse, Richard H.; Green, Theron D.; Chambers, Richard C.; Jones, Marian W.

doi:10.1128/am.11.5.436-445.1963

Cited by 9 publications

References 34 publications

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The Surface Decontamination of Seeds to Produce Axenic Seedlings

Sweet

Bolton

1979

American J of Botany

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A variety of sterilizing agents were tested to develop a standard procedure for surface decontaminating seeds to produce axenic seedlings. The use of calcium hypochlorite (0.5% phosphate buffer, pH 6) for 10 min followed by three sterile water rinses was among the most effective agents, and it did not injure some species as did sodium hypochlorite, formaldehyde, ethylene oxide and mercuric chloride. Some species contained internal microbes requiring severe treatments which killed or injured the seedling, while other species were “decontaminated” with a sterile water rinse. The percentage of seeds with internal microbes varied considerably among plant species, seed lot, and the length of seed storage. Thus, with seeds not easily decontaminated, screening of additional seed lots would be more profitable than testing additional decontamination agents. Release of microbes from the seed's interior is associated with germination, and microbial testing must last at least 11 days. Nutrient agar permitted growth, although the seedlings outgrew petri plates too quickly for adequate certification. These seedlings were transferred to nutrient agar in quart jars in which an internal pool of broth was periodically agitated to permit microbial sampling of the leaves while the plant grew.

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Disinfectants In Interventional Practices

Aranke

Moheimani

Phuphanich

et al. 2021

Curr Pain Headache Rep

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Purpose of Review This review aims to provide relevant, aggregate information about a variety of disinfectants and antiseptics, along with potential utility and limitations. While not exhaustive, this review’s goal is to add to the body of literature available on this topic and give interventional providers and practitioners an additional resource to consider when performing procedures. Recent Findings In the current SARS-CoV2 epidemiological environment, infection control and costs associated with healthcare-associated infections (HAIs) are of paramount importance. Even before the onset of SARS-CoV2, HAIs affected nearly 2million patients a year in the USA and resulted in nearly 90,000 deaths, all of which resulted in a cost to hospitals ranging from US$28 billion to 45 billion. The onset SARS-CoV2, though not spread by an airborne route, has heightened infection control protocols in hospitals and, as such, cast a renewed focus on disinfectants and their utility across different settings and organisms. Summary The aim of this review is to provide a comprehensive overview of disinfectants used in the inpatient setting.

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Disinfection of Aerosolized Pathogenic Fungi on Laboratory Surfaces

Cited by 9 publications

References 34 publications

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The Surface Decontamination of Seeds to Produce Axenic Seedlings

Disinfectants In Interventional Practices

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