Disinfection of an <scp>EMS</scp>/<scp>AHPND</scp> strain of <i>Vibrio parahaemolyticus</i> using ozone nanobubbles

Imaizumi, Kentaro; Tinwongger, Sasiwipa; Kondo, Hidehiro; Hirono, Ikuo

doi:10.1111/jfd.12783

Cited by 29 publications

(29 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Application of ozone gas using nanobubble technology is relatively new to aquaculture. A previous study reported the sterilization efficacy of NB-O3 against pathogenic V. parahaemolyticus, a Gram negative bacteria causing disease in marine shrimp (Imaizumi et al, 2018). In this study, we first revealed that NB-O3 has disinfection property against two common bacterial pathogens of freshwater farmed tilapia, S. agalactiae and A. veronii.…”

Section: Discussionmentioning

confidence: 77%

“…However, in their study NB-O3 showed a negative effect on shrimp when administered at a high level (970 mV ORP). When the NB-O3 treated water was diluted by 50% and the results revealed that all shrimp exposing to pathogenic V. parahaemolyticus survived from the bacterial infection, while all shrimp died in the group without the NB-O3 treatment (Imaizumi et al, 2018).…”

Section: Introductionmentioning

confidence: 98%

“…Most importantly, this treatment condition was safe for both sea cucumbers (Apostichopus japonicas) and sea urchins (Strongylocentrotus intermedius), which are commonly infected with these pathogenic crustaceans in Japanese aquaculture systems. In another study, Imaizumi et al (2018) reported that NB-O3 could be used for disinfection of Vibrio parahaemolyticus, a unique strain causing early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) in whiteleg shrimp (Penaeus vannamei). However, in their study NB-O3 showed a negative effect on shrimp when administered at a high level (970 mV ORP).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ozone nanobubble treatment effectively reduced pathogenic Gram positive and negative bacteria in freshwater and safe for tilapia

Jhunkeaw

Khongcharoen

Rungrueng

et al. 2020

Preprint

View full text Add to dashboard Cite

19High concentrations of pathogenic bacteria in water usually results in outbreaks of bacterial 20 diseases in farmed fish. Here, we explored the potential application of an emerging nanobubble 21 technology in freshwater aquaculture. Specifically, we aimed to determine if this technology was 22 effective at reducing the concentration of pathogenic bacteria in the water, and to assess whether 23 it was safe for fish. An ozone nanobubble (NB-O3) treatment protocol was established based on 24 examination of nanobubble size, concentration, disinfection property, and impact on fish health. 25A 10-min treatment with NB-O3 in 50 L water generated approximately 2-3 × 10 7 bubbles with 26 majority sizes less than 130 nm and ozone level of ~800 mV ORP. A single treatment with water 27 contaminated with either Streptococcus agalactiae or Aeromonas veronii effectively reduced 28 96.11-97.92 % of the bacterial load. This same protocol was repeated 3 times with 99.93-99.99 % 29 reduction in the bacterial concentration. In comparison, bacterial concentration the control tanks 30 remained the same level during the experiments. In fish-cultured water with the presence of 31 organic matter (e.g. mucus, feces, bacterial flora, feed, etc.), the disinfection property of NB-O3 32 was reduced i.e bacterial concentration was reduced by 42.94 %, 84.94 % and 99.27 % after the 33 first, second and third treatments, respectively. To evaluate the safety of NB-O3 to fish, juvenile 34Nile tilapia were exposed to NB-O3 treatment for 10 minutes. No mortality was observed during 35 the treatment or 48 h post treatment. Gill histology examination revealed that a single NB-O3 36 treatment caused no alteration morphology. However, damage in the gill filaments was noticed in 37 the fish receiving two or three consecutive exposures within the same day. Results of all the 38 experiments conducted in this study suggest that NB-O3 technology is promising for controlling 39 pathogenic bacteria in aquaculture systems, and may be useful at reducing the risk of bacterial 40 disease outbreaks in farmed fish. 41

show abstract

Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ozone nanobubble treatment effectively reduced pathogenic Gram positive and negative bacteria in freshwater and safe for tilapia

Jhunkeaw

Khongcharoen

Rungrueng

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Kurita et al (2017) reported that NB-O 3 treatment significantly reduced planktonic crustacean parasites (63%) in juvenile sea cucumbers ( Apostichopus japonicas ) and sea urchins ( Strongylocentrotus intermedius ). In another study, NB-O 3 demonstrated good disinfection of Vibrio parahaemolyticus in water, and prevention of acute hepatopancreatic necrosis disease (AHPND) in whiteleg shrimp (Imaizumi et al, 2018). We found that NB-O 3 treatment (1-2 × 10 7 bubbles/mL) reduced the level of Streptococcus agalactiae and Aeromonas veronii in water by more than 97% and made it relatively safe for juvenile Nile tilapia (Jhunkeaw et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Oxygen nanobubbles (NB-O 2 ) have been used for improving dissolved oxygen (DO) in aquaculture systems, and promoting growth of Nile tilapia ( O. niloticus ) (Mahasri et al, 2018) and whiteleg shrimp ( Penaeus vannamei ) (Mauladani et al, 2020; Rahmawati et al, 2020). Recently, several studies have revealed that ozone nanobubbles (NB-O 3 ) show promise at reducing quantities of pathogenic bacteria and improving DO in water, as well as modulating the immune systems against bacterial infections (Imaizumi et al, 2018; Jhunkeaw et al, 2021; Linh et al, 2021; Nghia et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multidrug-resistantAeromonas hydrophila

Dien

Linh

Sangpo

et al. 2021

Preprint

View full text Add to dashboard Cite

Multidrug-resistant (MDR) bacteria has rapidly increased in aquaculture, which highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB-O3) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple treatments with direct NB-O3 exposures caused alterations to the gills of exposed-fish. Here, we set up a modified recirculation system (MRS) assembled with an NB-O3 device (MRS-NB-O3) to investigate whether MRS-NB-O3 were 1) safe for tilapia (Oreochromis niloticus), 2) effective at reducing bacterial load in rearing water, and 3) improved survivability of Nile tilapia following an immersion challenge with a lethal dose of MDR Aeromonas hydrophila. The results indicated no behavioral abnormalities or mortality of Nile tilapia during the 14-day study using the MRS-NB-O3 system. In the immersion challenge, although high bacterial concentration (~2 × 107 CFU/mL) was used, multiple NB-O3 treatments in the first two days reduced the bacteria between 15.9% to 35.6% of bacterial load in water while bacterial concentration increased 13.1% to 27.9% in the untreated control. There was slight up-regulation of non-specific immune-related genes in the gills of the fish receiving NB-O3 treatments. Most importantly, this treatment significantly improved survivability of Nile tilapia with relative percent survival (RPS) of 64.7 - 66.7% in treated fish and surviving fish developed specific antibody against MDR A. hydrophila. In summary, the result suggests that NB-O3 is a promising alternative to antibiotics to control bacterial diseases, including MDR bacteria, and has high potential for application in recirculation aquaculture system (RAS).

show abstract

Transcriptome Analysis Provides New Insights into Host Response to Hepatopancreatic Necrosis Disease in the Black Tiger Shrimp Penaeus monodon

Zhao

Chen

et al. 2021

J. Ocean Univ. China

View full text Add to dashboard Cite

Disinfection of an EMS/AHPND strain of Vibrio parahaemolyticus using ozone nanobubbles

Cited by 29 publications

References 21 publications

Ozone nanobubble treatment effectively reduced pathogenic Gram positive and negative bacteria in freshwater and safe for tilapia

Ozone nanobubble treatment effectively reduced pathogenic Gram positive and negative bacteria in freshwater and safe for tilapia

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multidrug-resistantAeromonas hydrophila

Transcriptome Analysis Provides New Insights into Host Response to Hepatopancreatic Necrosis Disease in the Black Tiger Shrimp Penaeus monodon

Contact Info

Product

Resources

About