Disinfection of dentinal tubules with 2% Chlorhexidine gel, Calcium hydroxide and herbal intracanal medicaments against Enterococcus faecalis : An in-vitro study

Vasudeva, Agrima; Sinha, Dakshita Joy; Tyagi, Shashi Prabha; Singh, Narendra Nath; Garg, Paridhi; Upadhyay, Dr. Deepti

doi:10.1016/j.sdj.2017.06.001

Cited by 43 publications

(38 citation statements)

References 24 publications

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“…The proficiency of E. faecalis to infiltrate dentinal tubules even up to a depth of 1000 µm is accredited to efaA and ace, bacterial adhesion factors. [ 3 ]…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of antimicrobial efficacy of calcium hydroxide along with proton pump inhibitor against Enterococcus faecalis

Divakar¹,

Mohan²,

Pulyodan³

et al. 2020

J Pharm Bioall Sci

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A BSTRACT Aim: The aim of this study was to evaluate in vitro the efficacy of pantoprazole, a proton pump inhibitor, alone, and in combination with calcium hydroxide against Enterococcus faecalis , a star survivor in endodontic pathology. Materials and Methods: Teeth were inoculated with E. faecalis strains MTCC 439 and were divided into groups. Group 1 used saline as the negative control, Group 2 used calcium hydroxide, Group 3 used pantoprazole alone, and Group 4 used calcium hydroxide along with pantoprazole. They were incubated and dentine harvesting was performed. Colonies were counted using a digital counting machine. Data were statistically analyzed. Results: Group 4 was found to be the most effective against the pathogen. Discussion: This study indicates that the association of calcium hydroxide with the proton pump inhibitor pantoprazole can be successfully used as an intracanal medicament against E. faecalis .

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“…The proficiency of E. faecalis to infiltrate dentinal tubules even up to a depth of 1000 µm is accredited to efaA and ace, bacterial adhesion factors. [ 3 ]…”

Section: Discussionmentioning

confidence: 99%

“…The specimens were irrigated with 10 mL of sterile saline at the end of 21 days to remove the incubation broth. [ 3 ] They were assigned into the remaining groups.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

Evaluation of antimicrobial efficacy of calcium hydroxide along with proton pump inhibitor against Enterococcus faecalis

Divakar¹,

Mohan²,

Pulyodan³

et al. 2020

J Pharm Bioall Sci

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“…The results of colony count showed that H-Av mixture at a concentration of 25% completely inhibited the growth of the studied bacteria. In studies examining the antibacterial effect of Aloe vera and chlorhexidine against E.faecalis, chlorhexidine has shown better results than aloe vera (24)(25)(26). In the agar well diffusion test, H-Av mixture at a concentration of 25% did not show significant difference in the diameter of inhibitory zone compared to 2% chlorhexidine.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of antibacterial effect of combination of honey and Aloe vera extract against Enterococcus faecalis and Escherichia coli

Motahari

Kafil

Bonab

2021

ABCMed

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Antimicrobial agents are used as intra-canal drugs or canal cleaners for complete dental root canal cleaning. This new study attempts to compare the antibacterial effect of 2% chlorhexidine and combination of honey and Aloe vera extract (H-Av mixture) against Enterococcus faecalis (E.faecalis) and Escherichia coli (E.coli) which are the most important pathogens isolated from infectious root canals. Enterococcus faecalis (ATCC® 29212™) and Escherichia coli (ATCC® 25922™) were cultured in the tryptic soy broth medium. Dilution of H-Av mixture by the method of macrodilution to determine Minimum Inhibitory Concentration was investigated. In this paper agar well diffusion and colony count methods were also used to ensure the accuracy of the results. The results were statistically analyzed by student’s t-test. The significant level established at 5% (P<0.05). The results of the methods of macrodilution, colony count and Agar well diffusion confirm the inhibitory effect of H-Av mixture on E.coli and E. fecalis. Statistically, there was no significant difference between the antibacterial effect of 2% chlorhexidine and 25% H-Av mixture (P>0.05) Increasing drug resistance to antimicrobial compounds needs to study of new drugs against pathogens. H-Av mixture with benefits such as availability, good taste and easier use than chlorhexidine, and fewer side effects can be a good option for intracanal irrigation after clinical trials.

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“…Canal lumen 57741:9165 + 4885:35226 0.02 Dentinal chips 64333:3333 + 6468:72819 7 BioMed Research International intracanal medicaments [35,[77][78][79], a more significant effort is done to develop materials that can eliminate EF from the root canal system completely.…”

Section: S3mentioning

confidence: 99%

Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics

Abdulrahman

Faraj

Dizaye

2020

BioMed Research International

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Background and Objectives. Multiple antibacterial agents have been mixed and used as an intracanal medicament-like modified triple antibiotic paste (MTAP) to eliminate Enterococcus faecalis (EF), which has been most frequently identified in the cases of failed root canal treatment and periapical lesions. This study is aimed at using a single antibacterial agent, nitrofurantoin (Nit), as an experimental intracanal medicament paste against different clinical isolates of EF bacteria and at comparing its antimicrobial efficacy with MTAP. Materials and Methods. Three strains of EF (S1, S2, and S3) were clinically isolated. A total of 198 straight single-rooted human teeth were collected and divided randomly into three main groups: group N (Nit) (n=90), group M (MTAP) (n=90), and group W (distilled water) (n=18). The main groups were subdivided into three subgroups according to the strain of EF: in groups N and M, subgroups S1, S2, and S3 (n=30), while in group W, subgroups S1, S2, and S3 (n=6). Then, each subgroup of N and M was divided into five groups (n=6) according to the concentrations of Nit or MTAP (6.25, 12.5, 25, 50, and 100 mg/mL). The colony-forming unit (CFU) of EF from the canal lumen and dentinal chips was measured. Results. Nit could eradicate S1, S2, and S3 completely with concentrations of 6.25, 12.5, and 25 mg/mL, respectively, while MTAP showed complete eradication of the three strains only at 25 mg/mL. In all the groups, it was found that the CFU counts of EF in the dentinal chips were higher than those in the root canal lumen. Conclusion. At the concentration of 25 mg/mL, the Nit paste is effective in eradicating EF completely when it is used as an intracanal medicament.

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Disinfection of dentinal tubules with 2% Chlorhexidine gel, Calcium hydroxide and herbal intracanal medicaments against Enterococcus faecalis : An in-vitro study

Abstract: 2% Chlorhexidine gel gave the best results. Among the herbal extracts Propolis and Curcuma longa hold a promising future but to implement their use as sole intracanal medicaments clinically, further in vivo and long term studies are warranted.

Cited by 43 publications

References 24 publications

Evaluation of antimicrobial efficacy of calcium hydroxide along with proton pump inhibitor against Enterococcus faecalis

Evaluation of antimicrobial efficacy of calcium hydroxide along with proton pump inhibitor against Enterococcus faecalis

In vitro evaluation of antibacterial effect of combination of honey and Aloe vera extract against Enterococcus faecalis and Escherichia coli

Assessment of Nitrofurantoin as an Experimental Intracanal Medicament in Endodontics

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