Disinfection of human skin allografts in tissue banking: a systematic review report

Johnston, C.; Callum, Jeannie; Mohr, Jim; Duong, Andrew; Garibaldi, Alisha; Simunovic, Nicole; Ayeni, Olufemi R.

doi:10.1007/s10561-016-9569-2

Cited by 25 publications

(26 citation statements)

References 16 publications

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“…Furthermore, disinfectant-mediated viral inactivation cannot be used for corneal and cardiovascular tissue. The use of peracetic acid (0.1%; with or without 96% ethanol) is a validated method for sterilizing bone (Mohr et al 2016) and skin (Johnston et al 2016). However, some virus is highly resistant to this treatment (Pruss et al 2001).…”

Section: Disinfectantsmentioning

confidence: 99%

Effect of different human tissue processing techniques on SARS-CoV-2 inactivation-review

2020

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The safety of the tissue transplant recipient is a top priority for tissue banks, and the emergence of the new coronavirus SARS-CoV-2 has raised significant concerns about the risks of releasing tissue for clinical use. In the present study, we conducted a literature review about the potential infectivity of SARS-CoV-2 in different biological tissues and the influence of various tissue processing and sterilization procedures on viral inactivation. The search revealed that SARS-CoV-2 binds to the human angiotensinconverting enzyme receptor to penetrate human cells. These receptors are present in skin cells, musculoskeletal tissue, amniotic membranes, cardiovascular tissue and ocular tissues, including the cornea. In general, we found that coronaviruses are stable at low temperatures, and inactivated upon exposure to extreme heat and pH. Notably, gamma irradiation, which has already been employed to inactivate SARS and MERS, could be useful for sterilizing skin, amnion and musculoskeletal tissues against SARS-CoV-2. We conclude that due to the limited information about the effects of physical and chemical tissue processing methods on viral neutralization, rigorous donor screening is still essential for tissue transplant recipient safety.

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Section: Disinfectantsmentioning

confidence: 99%

Effect of different human tissue processing techniques on SARS-CoV-2 inactivation-review

2020

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“…Soaking in antibiotic or antimicrobial solutions is widely used to decontaminate viable tissues that cannot be terminally sterilized. 36,[39][40][41][42][43] To maintain cell integrity and tissue structure, skin grafts should be incubated in cryoprotectants, cooled gradually and thawed rapidly when needed for clinical use. [44][45][46] Cryopreserved skin grafts are stored at −130°C in vapor-phase nitrogen or at −196°C in liquid nitrogen.…”

Section: Cryopreservation and Freezingmentioning

confidence: 99%

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Tognetti¹,

Pianigiani²,

Ierardi³

et al. 2017

BSAM

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Donor skin and dermal grafts are used in several types of loss of substance for different clinical purposes. As biological physiological medication, donor skin grafts can promote re-epithelization, shorten healing time, alleviate pain and protect dermal and subcutaneous structures such as cartilage, tendons, bones and nerves. Though a great variety of dermal matrices and skin equivalents, both synthetic and semisynthetic, are now available for wound treatment, viable human skin allografts remain an important therapeutic choice for extensive deep burns and hard-to-heal wounds. In such cases, viable skin allografts have significantly better clinical outcomes than unviable human-derived allografts or synthetic medications. The demand for human-derived skin bioproducts continues to be a reason for the existence of skin banks. Skin bank organization is complex and requires continuous updating. Careful donor selection, thorough microbiological and serological donor screening for transmissible diseases and rigorous quality control during tissue preparation are necessary to minimize the risk of transmission of pathogenic agents. Skin banks must also observe standardized reproducible procedures to ensure tissue traceability and biological safety in all phases of processing and to avoid new biological contamination. Constant training and periodic checks are needed to keep skin bank operators attentive and responsible. Finally, skin banks should guarantee collection and storage of highly viable skin. Here, we discuss available tissue storage methods and the different types of skin bioproducts.

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“…Tissue procurement is recognised as presenting a major risk of contamination in cell therapy protocols [30]. However, the development of strict skin decontamination regimens prior to tissue harvesting and novel, closed harvesting devices to enable transfer of the source tissue begin to address this issue [31,32].…”

Section: From Lab-scale To Automated Large-scale Manufacturing Processesmentioning

confidence: 99%

Advances in automation for the production of clinical-grade mesenchymal stromal cells: the AUTOSTEM robotic platform

Ochs¹,

Barry²,

Schmitt³

et al. 2017

Cell Gene Therapy Insights

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Stem cell-based therapies are a central element of regenerative medicine and provide new treatment modalities for chronic and life-threatening conditions. Mesenchymal stem/stromal cells (MSCs) represent an important technology in regenerative medicine, although less developed with respect to clinical translation than hematopoietic stem cells (HSCs). MSC therapies may be based on the potential of the cells to differentiate to mesenchymal lineages or on their paracrine effects on host tissue. Both autologous and allogeneic applications are possible, the latter enabled by the low immunogenicity of the cells. Although stem cell therapy holds much promise for the treatment of chronic and debilitating diseases, there are still many obstacles to be overcome. In addition to the compelling need to generate strong and unambiguous clinical evidence, there are major technical gaps that must be filled. Chief among these is the development of manufacturing platforms for cell products that are efficient, cost effective and reproducible. Automated, robotic and closed production systems will provide the most efficient manufacturing strategy. Here we describe advances in automation for the clinical-scale production of MSCs and challenges associated with translating from lab-scale to automated large-scale manufacturing processes.

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Disinfection of human skin allografts in tissue banking: a systematic review report

Cited by 25 publications

References 16 publications

Effect of different human tissue processing techniques on SARS-CoV-2 inactivation-review

Effect of different human tissue processing techniques on SARS-CoV-2 inactivation-review

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Advances in automation for the production of clinical-grade mesenchymal stromal cells: the AUTOSTEM robotic platform

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