Disinfection of Perinereis aibuhitensis eggs with peroxymonosulfate to eliminate covert mortality nodavirus (CMNV)

Yang, Mingshu; Zeng, Chao; Xia, Jitao; Liu, Qinghui; Fang, Jinghui; Zhang, Qingli

doi:10.1016/j.aquaculture.2023.739539

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…However, little attention has been paid to the marine mudflat organisms known as Perinereis aibuhitensis (P. aibuhitensis), which also belong to Annelida (phylum). They are often present as bait in aquaculture [22]. They have been explored as research subjects to investigate the potential impacts of marine pollutants [23].…”

Section: Introductionmentioning

confidence: 99%

Purification and Properties of a Plasmin-like Marine Protease from Clamworm (Perinereis aibuhitensis)

Jiang,

Zhang,

Zhang

et al. 2024

Marine Drugs

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Marine organisms are a rich source of enzymes that exhibit excellent biological activity and a wide range of applications. However, there has been limited research on the proteases found in marine mudflat organisms. Based on this background, the marine fibrinolytic enzyme FELP, which was isolated and purified from clamworm (Perinereis aibuhitensis), has exhibited excellent fibrinolytic activity. We demonstrated the FELP with a purification of 10.61-fold by precipitation with ammonium sulfate, ion-exchange chromatography, and gel-filtration chromatography. SDS-PAGE, fibrin plate method, and LC–MS/MS indicated that the molecular weight of FELP is 28.9 kDa and identified FELP as a fibrinolytic enzyme-like protease. FELP displayed the maximum fibrinolytic activity at pH 9 (407 ± 16 mm2) and 50 °C (724 ± 27 mm2) and had excellent stability at pH 7–11 (50%) or 30–60 °C (60%), respectively. The three-dimensional structure of some amino acid residues of FELP was predicted with the SWISS-MODEL. The fibrinolytic and fibrinogenolytic assays showed that the enzyme possessed direct fibrinolytic activity and indirect fibrinolysis via the activation of plasminogen; it could preferentially degrade Aα-chains of fibrinogen, followed by Bβ- and γ-chains. Overall, the fibrinolytic enzyme was successfully purified from Perinereis aibuhitensis, a marine Annelida (phylum), with favorable stability that has strong fibrinolysis activity in vitro. Therefore, FELP appears to be a potent fibrinolytic enzyme with an application that deserves further investigation.

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Section: Introductionmentioning

confidence: 99%

Purification and Properties of a Plasmin-like Marine Protease from Clamworm (Perinereis aibuhitensis)

Jiang,

Zhang,

Zhang

et al. 2024

Marine Drugs

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“…For example, Devauchelle reported the discovery of Nereis iridescent virus (NIV) from Nereis diversicolor through electron microscopy [ 25 ]. However, the exploration of unknown viruses within the Perinereis genus remains limited [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. This gap in knowledge underscores the importance of preemptively identifying and understanding novel Perinereis viruses to mitigate potential economic losses due to disease outbreaks.…”

Section: Introductionmentioning

confidence: 99%

Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes Perinereis

Luo,

Zhang,

Zhou

et al. 2024

Veterinary Sciences

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Perinereis species are essential benthonic animals in coastal ecosystems and have significant roles as live feed in aquaculture, owing to their high-protein and low-fat nutritional profile. Despite their ecological importance, the viral communities associated with these organisms need to be better understood. In this study, we generated 2.6 × 108 reads using meta-transcriptomic sequencing and de novo assembled 5.3 × 103 virus-associated contigs. We identified 12 novel RNA viruses from two species, Perinereis aibuhitensis and P. wilsoni, which were classified into four major viral groups: Picobirnaviridae, Marnaviridae, unclassified Picornavirales, and unclassified Bunyavirales. Our findings revealed the hidden diversity of viruses and genome structures in Perinereis, enriching the RNA virosphere and expanding the host range of Picobirnaviridae, Marnaviridae, and Bunyavirales. This study also highlighted the potential biosecurity risk of the novel viruses carried by Perinereis to aquaculture.

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Evaluation of combined disinfection methods of infectious myonecrosis virus (IMNV) in seawater using Pacific white shrimp (Penaeus vannamei)

Yang,

Wang,

et al. 2024

Aquaculture

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Disinfection of Perinereis aibuhitensis eggs with peroxymonosulfate to eliminate covert mortality nodavirus (CMNV)

Cited by 3 publications

References 30 publications

Purification and Properties of a Plasmin-like Marine Protease from Clamworm (Perinereis aibuhitensis)

Purification and Properties of a Plasmin-like Marine Protease from Clamworm (Perinereis aibuhitensis)

Meta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes Perinereis

Evaluation of combined disinfection methods of infectious myonecrosis virus (IMNV) in seawater using Pacific white shrimp (Penaeus vannamei)

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