Disinfection protocol for human musculoskeletal allografts in tissue banking using hydrogen peroxide 30%

Dantas, Letícia Ramos; Wollmann, Luciana Cristina; Suss, Paula Hansen; Kraft, Letícia; Ribeiro, Victoria Stadler Tasca; Tuon, Felipe Francisco

doi:10.1007/s10561-021-09938-4

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…The discs of 6 mm in diameter and 3 mm thick (0.084 mL) (retrieved from a sagittal cut in the bone using a table band saw, using 6 mm diamond drill and small bone cylinders) (Figure 2) were made. Then, using a 1 2 inch chisel, a hammer and a plastic caliper, the discs were measured and cut and processed using a modified, previously described protocol [16]. The procedure used to AgNPs synthesis was adapted from Dong et al [17], where 8.5 mg of silver nitrate was dissolved (AgNO 3 ) in 45 mL of deionized water, followed by the addition of 14.7 mg of trisodium citrate (Na 3 C 6 H 5 O 7 ).…”

Section: Bone Scaffolds' Processing Nanoparticles' Synthesis and Agnp...mentioning

confidence: 99%

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms

Gonçalves,

Ribeiro,

Dantas

et al. 2023

Compounds

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Introduction: The antibiofilm activity of silver nanoparticles has been extensively investigated in common bacteria. Metallo-β-lactamase-producing Gram-negative bacteria are hard-to-treat microorganisms with few therapeutic options, and silver nanoparticles were not evaluated on the biofilm of these bacteria. Objectives: The aim of this study was to evaluate the antibiofilm activity of a bone scaffold impregnated with silver nanoparticles in NDM-producing Gram-negative bacilli. Methods: Bone scaffolds from bovine femur were used for the tests and impregnated with silver nanoparticles (50 nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentrations (MIC and MBC) were performed on NDM-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Disc diffusion tests for silver nanoparticles’ susceptibility and the quantification of biofilm production on plate and bone with sessile cell count were performed. Results: The MIC results demonstrated that silver nitrate had an antimicrobial effect on all microorganisms, inactivating the growth of isolates from a concentration of 8 µg/mL. MBC results showed that E. coli 16.211 was the only isolate to present MIC that were different from MBC, with a value of 16 µg/mL. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce the biofilm of multidrug-resistant bacteria. This is a strategical material that can be used as bone implant in different clinical conditions.

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Section: Bone Scaffolds' Processing Nanoparticles' Synthesis and Agnp...mentioning

confidence: 99%

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms

Gonçalves,

Ribeiro,

Dantas

et al. 2023

Compounds

Self Cite

View full text Add to dashboard Cite

show abstract

“…The discs with 6 mm in diameter and 3 mm thick (0.084 mL) (retrieved from a sagittal cut in the bone using a table band saw, using 6 mm diamond drill, small bone cylinders) (Figure 1) were made. Then, using a ½ inch chisel, a hammer and a plastic caliper, the discs were measured and cut) were processed using a modified protocol previously described [16]. The procedure used to AgNPs synthesis was adapted from Dong et al [17].…”

Section: Bone Scaffolds Processing Nanoparticles Synthesis and Agnps ...mentioning

confidence: 99%

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New-Delhi-Metallo-β-Lactamase Producing <em>Enterobacterales</em> Biofilms

Gonçalves,

Ribeiro,

Dantas

et al. 2023

Preprint

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Background: Antibiofilm activity of silver nanoparticles has been extensively investigated in common bacteria. Metallo-β-lactamase producing Gram-negative bacteria are hard-to-treat microorganisms with few therapeutic options, and silver nanoparticles were not evaluated on the biofilm of these bacteria. Objectives: The aim of this study was to evaluate the antibiofilm activity of a bone scaffold impregnated with silver nanoparticles in NDM-producing Gram-negative bacilli. Methods: Bone scaffolds from bovine femur were used for the tests and impregnated with silver nanoparticles (50 nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentrations (MIC and MBC) were performed on NDM-producing Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Disc diffusion tests for silver nanoparticles susceptibility and quantification of biofilm production on plate and bone with sessile cell count were performed. Results: The MIC results demonstrated that silver nitrate had an antimicrobial effect on all microorganisms, inactivating the growth of isolates from a concentration of 8 µg/mL. MBC results showed that E. coli 16.211 was the only isolate to present MIC different from MBC, with a value of 16 µg/mL. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce biofilm, and it can be a strategic material to be used as an implant for different approaches.

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“…These steps were carried out to remove various components such as bone marrow, blood cells, lipids, proteins, and nucleic acids. Each of these steps is detailed in a previous publication from our research group [10]. For processing, the volume of each washing solution was calculated at a ratio of eight samples to 10 mL of washing solution.…”

Section: Bone Processingmentioning

confidence: 99%

Antimicrobial Activity of Bone Scaffolds Impregnated with Silver Nanoparticles on Bacterial and Fungal Biofilm

Dantas,

Kraft,

Gonçalves

et al. 2023

Preprint

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Introduction: Silver nanoparticles have been extensively investigated in dental and orthopedic materials. However, the impregnation of bone graft with silver nanoparticles has been poorly investigated. Objectives: The aim of this study was to evaluate the in vitro antibiofilm activity of a bovine bone impregnated with silver nanoparticles. Methods: Bone scaffolds from cancellous bovine femur were used for the tests and impregnated with silver nanoparticles (50nm) by physical adsorption. Silver nitrate minimal inhibitory and bactericidal concentration were performed Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis, Acinetobacter baumannii, and Escherichia coli. Disc diffusion tests for silver nanoparticles susceptibility and quantification of biofilm production on plate and bone with sessile cell count were also performed. Results: All pathogens were susceptible to silver with low minimal inhibitory concentration (0.25 – 4 mg/L). The scaffold impregnated with silver nanoparticles presented a significant reduction in the biofilm cells for all microorganisms with a reduction of more than 3 logs in colony forming units count. Conclusion: Bone scaffolds impregnated with silver nanoparticles can significantly reduce biofilm, and it can be a strategical material to be used as an implant for different approaches.

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Disinfection protocol for human musculoskeletal allografts in tissue banking using hydrogen peroxide 30%

Cited by 6 publications

References 28 publications

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New Delhi Metallo-β-Lactamase-Producing Gram-Negative Bacilli Biofilms

Antimicrobial Activity of Bovine Bone Scaffolds Impregnated with Silver Nanoparticles on New-Delhi-Metallo-β-Lactamase Producing <em>Enterobacterales</em> Biofilms

Antimicrobial Activity of Bone Scaffolds Impregnated with Silver Nanoparticles on Bacterial and Fungal Biofilm

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