Disintegration of wet microalgae biomass with deep-eutectic-solvent-assisted hydrothermal treatment for sustainable lipid extraction

Abstract

Deep eutectic solvent (DES) with abundant hydrogen bond acceptors and donors was employed to promote disintegration of microalgae biomass with hydrothermal treatment (HTT) for green lipid extraction. The lipid extraction...

Efficient fractionation of microalgae biomass as a whole for biodiesel production enabled by deep eutectic solvent and water

“…The protein concentrations dissolved in the aqueous phase of DESs were 9.5 g/L (CC-FA), 7.1 g/L (CC-AA), and 9.2 g/L (CC-LA), respectively. This trend was observed to be the same as the π* value of DESs, because the high polarity solution helps protein solubilization [12]. In general, the fractionation e ciency showed that CC-LA had a better pretreatment effect, followed by CC-FA and CC-AA, which was veri ed by the sacchari cation e ciency of RMP (Fig.…”

“…Furthermore, DES can effectively destroy the hydrogen bond network on the cell wall of microalgae to achieve the purpose of cracking microalgae cells. Meanwhile, DES has a high a nity for proteins, and can quickly dissolve and extract a large number of proteins during the treatment of microalgae [12]. Importantly, the composition of DES can be freely adjusted to change its physicochemical properties.…”

Deep eutectic solvents as an emerging green platform for the synthesis of functional materials

“…The full utilization of biomass sugars and more efficient biomass conversion can only be achieved by greater recovery of glucan sugars and xylan. Huang et al 65 on the other hand, utilized DESs to treat wet microalgal biomass for lipid extraction. The hydrogen bonding network of the DES can disrupt intermolecular interactions and promote depolymerization of microalgal organisms to facilitate diffusive extraction of lipids.…”

Combination of a Deep Eutectic Solvent and Macroporous Resin for Green Recovery of Iridoids, Chlorogenic Acid, and Flavonoids from Eucommia ulmoides Leaves

“…The surface tension and viscosity of solvents can be impacted by variations in the molar ratio of choline chloride to hydrogen bond donors [43][44][45]. Thus, the impact of the DES composition molar ratio on the extraction yield was examined.…”

“…The RAW264.7 cell viability was assessed using the MTT assay [45]. Specifically, the RAW264.7 cell suspension (1 × 10 5 cells/mL) was pipetted into a 96-well plate (100 µL), where it was cultivated for 24 h at 310.15 K in a humid incubator with 5% CO 2 .…”