Let A and B be Banach algebras. A linear map T : A → B is called separating or disjointness preserving if ab = 0 implies T a T b = 0 for all a, b ∈ A. In this paper, we study a new class of regular Tauberian algebras and prove that some well-known Banach algebras in harmonic analysis belong to this class. We show that a bijective separating map between these algebras turns out to be continuous and the maximal ideal spaces of underlying algebras are homeomorphic. By imposing extra conditions on these algebras, we find a more thorough characterization of separating maps. The existence of a bijective separating map also leads to the existence of an algebraic isomorphism in some cases.