Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates

Jeon, Suhak; Shin, Jong Hee; Lim, Ha Jin; Choi, Min Ji; Byun, Seung A; Lee, Dain; Lee, Seung Yeob; Won, Eun Jeong; Kim, Soo Hyun; Shin, Myung Geun

doi:10.3343/alm.2021.41.6.559

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…The results showed a very good agreement rate between both methods (97.1%). These findings are in conformity with those conducted by Jeon et al, 2021 43 and Kumar et al, 2015 44 . They reported 97%, 100% categorical agreement between DD method and the BMD method for assessing FLZ susceptibility against C. albicans isolates 43,44 .…”

Section: Discussionsupporting

confidence: 93%

“…These findings are in conformity with those conducted by Jeon et al, 2021 43 and Kumar et al, 2015 44 . They reported 97%, 100% categorical agreement between DD method and the BMD method for assessing FLZ susceptibility against C. albicans isolates 43,44 . Kumar et al concluded that DD methods can be used as substitute for the reference BMD method for Candida spp susceptibility testing of FLZ 44 .…”

Section: Discussionsupporting

confidence: 93%

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Potential Anti-fungal Activity of Lactobacilli Probiotic Against Fluconazole Resistant Candida albicans Clinical Isolates

El-Ashmony

Raslan

Abdelhamid

et al. 2022

Azhar International Journal of Pharmaceutical and Medical Scien

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Fluconazole resistance by Candida albicans (C. albicans) is a growing medical issue which makes patient management more difficult. The development of new effective, safe and inexpensive alternatives becomes essential. Probiotics represent an intriguing strategy that can be applied for prevention or treatment of Candida infections. The aim of our study is to investigate the anti-fungal activity of different Lactobacillus species against fluconazole resistant C. albicans isolates and to evaluate the inhibitory effect of cell free supernatant of the most potent one. Sixty-nine C. albicans isolates were collected from different clinical specimens and subjected to antifungal susceptibility testing. Then, the anti-Candida activity of various Lactobacillus spp was investigated via different methods, spot overlay method, radial streak method and agar well diffusion method. Results showed that 33% of C. albicans isolates were resistant to fluconazole with MIC ≥ 64 μg/ml. L. rhamnosus demonstrated strong inhibitory activity against the majority of fluconazole resistant C. albicans isolates, followed by L. reuteri and L. salivarus respectively. Inhibitory activity of L. rhamnosus against C. albicans may be due to acid production. Our findings conclude that L. rhamnosus represent a promising candidate to be used in the treatment of candidiasis caused by fluconazole-resistant C. albicans strains.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Potential Anti-fungal Activity of Lactobacilli Probiotic Against Fluconazole Resistant Candida albicans Clinical Isolates

El-Ashmony

Raslan

Abdelhamid

et al. 2022

Azhar International Journal of Pharmaceutical and Medical Scien

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“…Furthermore, similar to our findings, recent studies have demonstrated that the DD antifungal susceptibility method showed a good agreement with the BMD method for Candida spp., including C. glabrata and C. auris [22,23]. However, the use of a larger inoculum may detect fluconazole-resistant Candida species more rapidly and reliably [24].…”

Section: Discussionmentioning

confidence: 99%

Impact of the Disk Diffusion Test on Fluconazole De-Escalation in Patients with Candidemia

Tantasuwan

Chongtrakool

Waesamaae

et al. 2022

JoF

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Disk diffusion (DD) is a simple antifungal susceptibility method for Candida. This study examined the impact of fluconazole DD testing on antifungal de-escalation. We enrolled patients with candidemia whose Candida isolates were tested for fluconazole susceptibility using DD between January 2019 and January 2020. The historical controls were patients with candidemia who underwent fluconazole susceptibility testing using the broth microdilution (BMD) method. Clinical data including antifungal therapy were analyzed. In total, 108 patients were enrolled. Most baseline characteristics were comparable between the groups. C. tropicalis was the predominant isolate (54.6%), followed by C. albicans (17.6%). The rates of antifungal de-escalation within 72 h were 25.9 and 9.3% in the DD and BMD groups, respectively (p = 0.023). The median time to de-escalation was 3 days in the DD group, versus 6 days in the BMD group (p = 0.037). The 14-day mortality rate and antifungal cost tended to be lower in the DD group. There were no differences in the length of hospital stay and treatment-related complications between the two groups. The agreement between the DD and BMD results was 90%. DD testing can be substituted for BMD to enhance antifungal de-escalation and antifungal stewardship.

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“…To investigate the antifungal effect of six synthesized ionic liquids, the disc diffusion method was used based on standard CLSI M44-A2 ( Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts) ( 16 ). After evaluating the ILs antifungal ability, the powerful IL against candida growth was selected for continued experimental.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans

Habibi,

Bayat,

Omidi

et al. 2023

IJM

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Background and Objectives: Candida albicans cause a problematic condition in immunocompromised patients that could not be treated quickly due to the resistant behavior of microorganisms. This study aimed to investigate the effect of a novel ionic liquid (IL) as a new drug on C. albicans strains. Materials and Methods: Seven newly binary ionic liquids mixtures were synthesized, and among them, ([prollinium chlo- ride] [1-methylimidazolium 3-sulfonate] ([pro-HCl][MImS]) was selected and characterized by 1HNMR, 13C NMR, and FT-IR methods. Samples from patients (n=50) with candidiasis were collected and identified through culture media. ERG11 gene overexpression was related to resistance against azole-bearing drugs. The antibiogram, well diffusion assay, MICs, and MFCs tests were operated. PCR and Real-time evaluated the expression of the ERG11 gene, and the rate of cell death was detected using Flow Cytometry. Results: Our data manifested that this novel IL (Ionic Liquid) can inhibit C. albican's growth, reduce the expression of ERG11 and increase dead cells. Conclusion: The newly synthesized IL had an inhibiting effect on the growth of the C. albicans strains and may be used as an alternative candidate for novel drug design.

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Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates

Cited by 6 publications

References 22 publications

Potential Anti-fungal Activity of Lactobacilli Probiotic Against Fluconazole Resistant Candida albicans Clinical Isolates

Potential Anti-fungal Activity of Lactobacilli Probiotic Against Fluconazole Resistant Candida albicans Clinical Isolates

Impact of the Disk Diffusion Test on Fluconazole De-Escalation in Patients with Candidemia

Synthesis of new imidazole-based ionic liquids with antifungal activity against Candida albicans

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