Disomic Segregation of Microsatellites in the Tetraploid Prunus serotina Ehrh. (Rosaceae)

Pairon, Marie; Jacquemart, Anne‐Laure

doi:10.21273/jashs.130.5.729

Cited by 18 publications

(12 citation statements)

References 31 publications

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“…(Saal et al, 2001), or Pyrus communis L. (Fernandez-Fernandez et al, 2006)], and the data can be analyzed as if the species were diploids. So far, five microsatellite markers have been described in black cherry (Downey and Iezzoni, 2000;Pairon and Jacquemart, 2005), among which two amplified both duplicated loci with overlapping expected allele ranges (M4c, Ps12a02), one amplified both loci with different allele ranges (pchpgms3), and two (pchgms2, PceGA34) were genome-specific (Pairon and Jacquemart, 2005).…”

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Detection and Characterization of Genome-specific Microsatellite Markers in the Allotetraploid Prunus serotina

Pairon¹,

Jacquemart²,

Potter³

2008

J. Amer. Soc. Hort. Sci.

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The utility of microsatellite markers to characterize the genetic diversity of a polyploid species with disomic inheritance is often hampered by the impossibility of determining allele frequencies and the complexity of inheritance patterns. The objective of this study was to solve these problems in the allotetraploid Prunus serotina Ehrh. by finding genome-specific primers (i.e., primers that are specific to one of the two genomes that initially formed the species). Sixty-seven microsatellite primers described in cultivated Prunus L. species were tested for cross-amplification in P. serotina, and evidence that conserved markers were genome-specific was found by demonstrating their typical Mendelian diploid inheritance in embryos resulting from controlled crosses. Among the 67 microsatellite markers tested, 26 produced successful amplification and five were genome-specific. No linkage disequilibrium was detected for these loci, but evidence was found for the presence of a null allele at one locus. We found both a high number of alleles per locus (three to 12) and a high mean expected heterozygosity (0.71), which were nonsignificantly different from the number of alleles and estimates of expected heterozygosity calculated for three non-genome-specific markers in the same population. The potential use of these genome-specific markers in population genetic studies is discussed.

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Detection and Characterization of Genome-specific Microsatellite Markers in the Allotetraploid Prunus serotina

Pairon¹,

Jacquemart²,

Potter³

2008

J. Amer. Soc. Hort. Sci.

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“…were specified. Although all samples are presumably allotetraploid (Pairon & Jacquemart, ; Pairon & Jaquemart, ; Stairs & Hauck, ), 10 of the 15 loci amplified ≤2 alleles per sample, presumably due to mutational differences at priming sites in the two constituent genomes. Both standard Mantel and Mantel correlogram analyses utilized the basic “Lynch” band‐sharing distance (Lynch, ) obtained in the R package “polysat” v.1.7 (Clark & Jasieniuk, ).…”

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confidence: 99%

A rangewide herbarium‐derived dataset indicates high levels of gene flow in black cherry (Prunus serotina)

Konrade

Shaw

Beck

2019

Ecology and Evolution

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Isolation by Distance (IBD) is a genetic pattern in which populations geographically closer to one another are more genetically similar to each other than populations which are farther apart. Black cherry (Prunus serotina Ehrh.) (Rosaceae) is a forest tree species widespread in eastern North America, and found sporadically in the southwestern United States, Mexico, and Guatemala. IBD has been studied in relatively few North American plant taxa, and no study has rigorously sampled across the range of such a widespread species. In this study, IBD and overall genetic structure were assessed in eastern black cherry (P. serotina Ehrh. var. serotina), the widespread variety of eastern North America. Eastern North America. Prunus serotina Ehrh. var. serotina (Rosaceae). Dense sampling across the entire range of eastern black cherry was made possible by genotyping 15 microsatellite loci in 439 herbarium samples from all portions of the range. Mantel tests and STRUCTURE analyses were performed to evaluate the hypothesis of IBD and genetic structure. Mantel tests demonstrated significant but weak IBD, while STRUCTURE analyses revealed no clear geographic pattern of genetic groups. The modest geographic/genetic structure across the eastern black cherry range suggests widespread gene flow in this taxon. This is consistent with P. serotina's status as a disturbance‐associated species. Further studies should similarly evaluate IBD in species characteristic of low‐disturbance forests.

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“…Bullock and Clarke 2000;Nathan et al 2000), degree of gene flow within a landscape (e.g. Ouborg et al 1999;Grace et al 2004;Jones et al 2005;Sato et al 2006;Hardesty et al 2006;Pairon and Jacquemart 2005) or different types of mechanistic models (e.g. Nathan et al 2001Nathan et al , 2002Tackenberg et al 2003;Soons et al 2004;Kuparinen et al 2007) to estimate possible dispersal distances.…”

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confidence: 99%

Spatial and temporal variation in dispersal pattern of an invasive pine

et al. 2009

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Understanding dispersal ability of an invasive species is crucial for describing its potential spread. Despite this, we still know little about the dispersal potential of many invasive species. We explored dispersal spectra in Pinus strobus, an invasive tree in sandstone areas in Central Europe. We studied dispersal of the species using distribution of self-sown trees in the field. We compared these observed data with theoretical dispersal curves derived using information on wind speed, seed terminal velocity and tree height. Finally, we fitted various empirical dispersal curves to the observed data. All the analyses based on the observed field data were done for the whole dataset, and for the dataset divided by habitat types and age categories of the self-sown trees. P. strobus seeds can disperse up to 757.5 m from the source. The observed data fall within the confidence intervals of the predictions based on a negative exponential model. When comparing different dispersal functions fitted to the data, it was not easy to decide which of the dispersal curves provides the overall best fit. This was because different functions were the best predictors of different parts of the dispersal curve. We suggest that future studies should provide not only empirical fitted dispersal curves but also observed data and provide estimates of confidence intervals. Such information will provide insights into the reliability of the dispersal estimates in general and help to evaluate the predictive power of the different models.

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Disomic Segregation of Microsatellites in the Tetraploid Prunus serotina Ehrh. (Rosaceae)

Cited by 18 publications

References 31 publications

Detection and Characterization of Genome-specific Microsatellite Markers in the Allotetraploid Prunus serotina

Detection and Characterization of Genome-specific Microsatellite Markers in the Allotetraploid Prunus serotina

A rangewide herbarium‐derived dataset indicates high levels of gene flow in black cherry (Prunus serotina)

Spatial and temporal variation in dispersal pattern of an invasive pine

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