Disordered protein networks as mechanistic drivers of membrane remodeling and endocytosis

Zeno, Wade F.; Feng, Yuan; Graham, Kristin; Stachowiak, Jeanne C.

doi:10.1016/b978-0-323-99533-7.00011-x

Cited by 4 publications

(5 citation statements)

References 121 publications

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“…Increasingly more evidence suggests that in spite of lacking any specific structural motifs, IDRs and IDPs can be potent sensors of membrane curvature. 56 Two mechanisms have been identified to account for the membrane curvature sensing by IDPs. 57 The first one is entropic in nature: IDPs bind preferentially to membrane regions curved outward because the more convex the membrane is, the larger the conformational entropy of membrane-associated IDPs becomes.…”

Section: Discussionmentioning

confidence: 99%

Membrane curvature sensing by model biomolecular condensates

2023

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Section: Discussionmentioning

confidence: 99%

Membrane curvature sensing by model biomolecular condensates

2023

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“…Increasingly more evidence suggests that in spite of lacking any specific structural motifs, IDRs and IDPs can be potent sensors of membrane curvature [60]. Two mechanisms have been identified to account for the membrane curvature sensing by IDPs [59].…”

Section: Discussionmentioning

confidence: 99%

Membrane curvature sensing by model biomolecular condensates

Anila

Ghosh

Różycki

2023

Preprint

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Biomolecular condensates (BCs) are fluid droplets that form in biological cells by liquid-liquid phase separation. Their major components are intrinsically disordered proteins. Vast attention has been given in recent years to BCs inside the cytosol and nucleus. BCs at the cell membrane have not been studied to the same extent so far. However, recent studies provide increasingly more examples of interfaces between BCs and membranes which function as platforms for diverse biomolecular processes. Galectin-3, for example, is known to mediate clathrin-independent endocytosis and has been recently shown to undergo liquid-liquid phase separation, but the function of BCs of galectin-3 in endocytic pit formation is unknown. Here, we use dissipative particle dynamics simulations to study a generic coarse-grained model for BCs interacting with lipid membranes. In analogy to galectin-3, we consider polymers comprising two segments -- one of them mediates multivalent attractive interactions between the polymers, and the other one has affinity for association with specific lipid head groups. When these polymers are brought into contact with a multi-component membrane, they spontaneously assemble into droplets and, simultaneously, induce lateral separation of lipids within the membrane. Interestingly, we find that if the membrane is bent, the polymer droplets localize at membrane regions curved inward. Although the polymers have no particular shape or intrinsic curvature, they appear to sense membrane curvature when clustered at the membrane. Our results indicate toward a generic mechanism of membrane curvature sensing by BCs involved in such processes as endocytosis.

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“…In addition to the disorder‐driven PPIs, the interaction of IDPs/IDRs with membranes is important in autophagy (Popelka & Uversky, 2022). The PPI involving disorder are in fact drive endocytosis and membrane remodeling (Wade et al, 2023). The bacterial tubulin‐like GTPase FtsZ that serves as the foundation for the cytokinetic ring at the nascent division site uses its intrinsically disordered C‐terminal domain for interaction with the membrane and modulation of the interactions both between FtsZ subunits and between FtsZ and modulatory proteins in the cytoplasm (Buske et al, 2015).…”

Section: Interactions Of Idps/idrs With Other Proteins and Surfaces I...mentioning

confidence: 99%

Protein structure–function continuum model: Emerging nexuses between specificity, evolution, and structure

Gupta,

Uversky

2024

Protein Science

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The rationale for replacing the old binary of structure–function with the trinity of structure, disorder, and function has gained considerable ground in recent years. A continuum model based on the expanded form of the existing paradigm can now subsume importance of both conformational flexibility and intrinsic disorder in protein function. The disorder is actually critical for understanding the protein–protein interactions in many regulatory processes, formation of membrane‐less organelles, and our revised notions of specificity as amply illustrated by moonlighting proteins. While its importance in formation of amyloids and function of prions is often discussed, the roles of intrinsic disorder in infectious diseases and protein function under extreme conditions are also becoming clear. This review is an attempt to discuss how our current understanding of protein function, specificity, and evolution fit better with the continuum model. This integration of structure and disorder under a single model may bring greater clarity in our continuing quest for understanding proteins and molecular mechanisms of their functionality.

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Disordered protein networks as mechanistic drivers of membrane remodeling and endocytosis

Cited by 4 publications

References 121 publications

Membrane curvature sensing by model biomolecular condensates

Membrane curvature sensing by model biomolecular condensates

Membrane curvature sensing by model biomolecular condensates

Protein structure–function continuum model: Emerging nexuses between specificity, evolution, and structure

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