Dispensability of Surfactant Proteins A and D in Immune Control of<i>Mycobacterium tuberculosis</i>Infection following Aerosol Challenge of Mice

Lemos, Maria P.; McKinney, John D.; Rhee, Kyu Y.

doi:10.1128/iai.00286-10

Cited by 24 publications

(23 citation statements)

References 49 publications

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“…and digested in dissociation media (21). Single-cell suspensions were counted in a hematocytometer and stained for surface flow cytometry (21).…”

Section: Methodsmentioning

confidence: 99%

“…and digested in dissociation media (21). Single-cell suspensions were counted in a hematocytometer and stained for surface flow cytometry (21). Fixed samples were collected on a FACSort Flow cytometer (BD) using CellQuest software and analyzed using FlowJo.…”

Section: Methodsmentioning

confidence: 99%

“…Lung cells were incubated for 5 h at 37°C in the presence of 5 ng/ml ESAT6 peptide (a kind gift from Dr. Eric Pamer) and GolgiStop (BD). Intracellular cytokine staining were performed as described previously (21). …”

Section: Methodsmentioning

confidence: 99%

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Expression of the Leptin Receptor outside of Bone Marrow-Derived Cells Regulates Tuberculosis Control and Lung Macrophage MHC Expression

Lemos

Rhee

McKinney

2011

The Journal of Immunology

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Leptin is a pleiotropic hormone proposed to link nutritional status to the development of strong Th1 immunity. As Mycobacterium tuberculosis (MTb) control is affected by starvation and diabetes, we studied the role of the leptin receptor in regulating distinct immune cells during chronic infection. Infected db/db mice, bearing a natural mutation in the leptin receptor, have a markedly increased bacterial load in their lungs when compared to their wild-type (WT) counterparts. In response to MTb infection, db/db mice exhibited disorganized granulomas, neutrophilia, and reduced B cell migration to the lungs, correlating with dysfunctional lung chemokine responses that include XCL1, CCL2, CXCL1, CXCL2, and CXCL13. In a db/db lung, myeloid cells were delayed in their production of inducible nitric oxide synthase (iNOS) and had reduced expression of MHC I and II. Although the Th1 cell response developed normally in the absence of leptin signaling, production of pulmonary IFNγ was delayed and ineffective. Surprisingly, a proper immune response took place in bone marrow (BM) chimeras lacking leptin receptor exclusively in BM-derived cells, indicating that leptin acts indirectly on immune cells to modulate the anti-tuberculosis response and bacterial control. Together these findings suggest that the pulmonary response to MTb is affected by the host’s nutritional status via the regulation of non-BM derived cells, and not through direct action of leptin on the Th1 immunity.

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“…and digested in dissociation media (21). Single-cell suspensions were counted in a hematocytometer and stained for surface flow cytometry (21).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Expression of the Leptin Receptor outside of Bone Marrow-Derived Cells Regulates Tuberculosis Control and Lung Macrophage MHC Expression

Lemos

Rhee

McKinney

2011

The Journal of Immunology

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“…The mortality of VACV-infected mice was increased in the absence of SP-D, suggesting the involvement of SP-D to neutralize VACV infection. Future experiments could be performed by measuring virus titers in lung, cells recruitment [48] [64], pro-inflammatory cytokine production [49] [48] and histopathological changes to explain the difference of mortality between SP-D -/- and wild-type mice and to address the significance of SP-D in protection against VACV infection.…”

Section: Resultsmentioning

confidence: 99%

Protective Effect of Surfactant Protein D in Pulmonary Vaccinia Virus Infection: Implication of A27 Viral Protein

Perino

Thielens

Crouch

et al. 2013

Viruses

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Vaccinia virus (VACV) was used as a surrogate of variola virus (VARV) (genus Orthopoxvirus), the causative agent of smallpox, to study Orthopoxvirus infection. VARV is principally transmitted between humans by aerosol droplets. Once inhaled, VARV first infects the respiratory tract where it could encounter surfactant components, such as soluble pattern recognition receptors. Surfactant protein D (SP-D), constitutively present in the lining fluids of the respiratory tract, plays important roles in innate host defense against virus infection. We investigated the role of SP-D in VACV infection and studied the A27 viral protein involvement in the interaction with SP-D. Interaction between SP-D and VACV caused viral inhibition in a lung cell model. Interaction of SP-D with VACV was mediated by the A27 viral protein. Binding required Ca2+ and interactions were blocked in the presence of excess of SP-D saccharide ligands. A27, which lacks glycosylation, directly interacted with SP-D. The interaction between SP-D and the viral particle was also observed using electron microscopy. Infection of mice lacking SP-D (SP-D-/-) resulted in increased mortality compared to SP-D+/+ mice. Altogether, our data show that SP-D participates in host defense against the vaccinia virus infection and that the interaction occurs with the viral surface protein A27.

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“…Constituting the first barrier Mtb encounters during infection, lung surfactant was shown to alter Mtb gene expression [158] and Mtb cell wall lipids change the function of lung surfactant [159,160]. SP-A and SP-D deficiency did not change the outcome of infection in mice [161], but the effect of lung surfactant on interactions between Mtb and macrophages is not fully understood.…”

Section: Recognition Recognition Recognitionmentioning

confidence: 99%

Interplay of human macrophages and Mycobacterium tuberculosis phenotypes

Raffetseder¹

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Dispensability of Surfactant Proteins A and D in Immune Control ofMycobacterium tuberculosisInfection following Aerosol Challenge of Mice

Cited by 24 publications

References 49 publications

Expression of the Leptin Receptor outside of Bone Marrow-Derived Cells Regulates Tuberculosis Control and Lung Macrophage MHC Expression

Expression of the Leptin Receptor outside of Bone Marrow-Derived Cells Regulates Tuberculosis Control and Lung Macrophage MHC Expression

Protective Effect of Surfactant Protein D in Pulmonary Vaccinia Virus Infection: Implication of A27 Viral Protein

Interplay of human macrophages and Mycobacterium tuberculosis phenotypes

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