Dispersion Behaviour of Silica Nanoparticles in Biological Media and Its Influence on Cellular Uptake

Halamoda-Kenzaoui, Blanka; Ceridono, Mara; Colpo, Pascal; Valsesia, Andrea; Urbán, Patricia; Ojea-Jiménez, Isaac; Gioria, Sabrina; Gilliland, Douglas; Rossi, François; Kinsner‐Ovaskainen, Agnieszka

doi:10.1371/journal.pone.0141593

Cited by 54 publications

(45 citation statements)

References 46 publications

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“…This can be attributed to the interaction of cationic AuNP with the negative surface charge on MDA MB 231 cells. Similar results have been reported in case of silica nanoparticles, wherein, it was observed that presence of cells changes the dispersion state of nanoparticle suspension 29 . The PCS and LDV analysis to estimate size and zeta potential of these agglomerates could not be performed as the protein content of media was very high and it caused a significant interference ( Figure 6A).…”

Section: Resultssupporting

confidence: 88%

Physicochemical changes in plasma membrane mirror nanoparticle-mediated cytotoxicity

Sood

Katti

2019

Preprint

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The aim of this chapter was to understand the influence of nanoparticle challenge on physicochemical characteristics of the cells and to correlate these changes with cytotoxic response of nanoparticles. A nanoparticle surface charge and a concentration-dependent cytotoxic response were observed in the breast cancer cell lines MDA MB 231 and SKBR3. The cationic gold nanoparticles were more cytotoxic to cells as compared to anionic gold nanoparticles. It was also observed that cationic nanoparticles compromised the integrity of the plasma membrane at higher concentrations. Cationic nanoparticle challenge also caused changes in physicochemical characteristics of plasma membrane.Higher concentration of cationic nanoparticles caused an irreversible change in the surface charge density of cells. However, anionic gold nanoparticles did not show any such effect. It was observed that the ROS-mediated oxidative stress was the mechanism of cationic gold nanoparticle-mediated cytotoxic effect. Mitochondrial depolarization was observed in both anionic and cationic nanoparticle challenge. Therefore, the role of mitochondrial ROS in nanoparticle-mediated cytotoxicity is questionable. Finally, a generalized model involving modulation of intracellular Ca 2+ can potentially provide an explanation for the observed pluralistic response of the cells towards nanoparticle challenge.

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Section: Resultssupporting

confidence: 88%

Physicochemical changes in plasma membrane mirror nanoparticle-mediated cytotoxicity

Sood

Katti

2019

Preprint

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“…As one can resolve from the images in Figure (see also Figure S5 in the Supporting Information), all white spots are localized outside cell membranes, while a granular shape of the corresponding blue spots in the SHG pattern suggests the aggregation of Si NPs in these regions. Such an aggregation in aqueous conditions via van der Waals forces was reported for many other nanoparticles (e.g., silica NPs) . It should be noted that SHG signals should be intense enough to detect only for large NP fragments (see our discussion below), confirming our supposition about the presence of large aggregations of Si NPs outside cell membranes.…”

supporting

confidence: 87%

Bi‐Modal Nonlinear Optical Contrast from Si Nanoparticles for Cancer Theranostics

Kharin

Lysenko

Rogov

et al. 2019

Advanced Optical Materials

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Having negligible toxicity compared to quantum dots based on compound semiconductors [1,2] and being capable of providing efficient imaging and therapeutic functionalities based on its unique physicochemical characteristics, [3][4][5] nanosilicon occupies a particularly important niche related to biological applications. The imaging functionality of nanosilicon typically employs photoluminescence (PL) of quantum-confined excitonic states in silicon (Si) nanocrystals (NCs) with sizes smaller than the exciton Bohr's radius (≈5 nm for the bulk crystalline Si (c-Si)), which enables tracking the presence of Si nanoparticles (NPs) in cells or tissues. [6][7][8][9][10][11][12][13] On the other hand, Si NPs can serve as efficient sensitizers of local heating under external stimuli to initiate hyperthermia-based therapies. As an example, Presenting a safe alternative to conventional compound quantum dots and other functional nanostructures, nanosilicon can offer a series of breakthrough hyperthermia-based therapies under near-infrared, radiofrequency, ultrasound, etc., excitation, but the size range to sensitize these therapies is typically too large (>10 nm) to enable efficient imaging functionality based on photoluminescence properties of quantum-confined excitonic states. Here, it is shown that large Si nanoparticles (NPs) are capable of providing two-photon excited luminescence (TPEL) and second harmonic generation (SHG) responses, much exceeding that of smaller Si NPs, which promises their use as probes for bi-modal nonlinear optical bioimaging. It is finally demonstrated that the combination of TPEL and SHG channels makes possible efficient tracing of both separated Si NPs and their aggregations in different cell compartments, while the resolution of such an approach is enough to obtain 3D images. The obtained bi-modal contrast provides lacking imaging functionality for large Si NPs and promises the development of novel cancer theranostic modalities on their basis.

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“…Besides, it is worth noting that there is a possibility of the formation of a protein corona, since the samples were dispersed in serum-enriched DMEM. It common in the literature that the corona formed around the nanoparticles lowers its cytotoxicity [43,45,46,47] by lowering its positively-charged surface. …”

Section: Resultsmentioning

confidence: 99%

Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles

et al. 2018

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Recently, flufenamic acid (FFA) was discovered among fenamates as a free radical scavenger and gap junction blocker; however, its effects have only been studied in cancer cells. Normal cells in the surroundings of a tumor also respond to radiation, although they are not hit by it directly. This phenomenon is known as the bystander effect, where response molecules pass from tumor cells to normal ones, through communication channels called gap junctions. The use of the enhanced permeability and retention effect, through which drug-loaded nanoparticles smaller than 200 nm may accumulate around a tumor, can prevent the local side effect upon controlled release of the drug. The present work, aimed at functionalizing MCM-41 (Mobil Composition of Matter No. 41) silica nanoparticles with FFA and determining its biocompatibility with human fibroblasts MRC-5 (Medical Research Council cell strain 5). MCM-41, was synthesized and characterized structurally and chemically, with multiple techniques. The biocompatibility assay was performed by Live/Dead technique, with calcein and propidium–iodide. MRC-5 cells were treated with FFA-grafted MCM-41 for 48 h, and 98% of cells remained viable, without signs of necrosis or morphological changes. The results show the feasibility of MCM-41 functionalization with FFA, and its potential protection of normal cells, in comparison to the role of FFA in cancerous ones.

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Dispersion Behaviour of Silica Nanoparticles in Biological Media and Its Influence on Cellular Uptake

Cited by 54 publications

References 46 publications

Physicochemical changes in plasma membrane mirror nanoparticle-mediated cytotoxicity

Physicochemical changes in plasma membrane mirror nanoparticle-mediated cytotoxicity

Bi‐Modal Nonlinear Optical Contrast from Si Nanoparticles for Cancer Theranostics

Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles

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