Display of CCL21 on cancer cell membrane through genetic modification using a pH low insertion peptide

Li, Sitian; Wang, Yongtao; Jiang, Huaiqing; Bai, Yitong; Chen, Tao; Chen, Min; Ma, Mengze; Yang, Shenyu; Wu, Yan; Shi, Chaochen; Wang, Fazhan; Chen, Yazhou

doi:10.1016/j.ijbiomac.2023.124324

Cited by 4 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, transcription cofactor YAP will give a response to biomechanical tension along with the stretched direction . YAP nuclear translocation may be related to cytoskeleton parallel stretch, FA kinase activation, and zyxin relocation by anisotropic cell nanomechanics. , Cell nanomechanics has been reported to suppress cell senescence and aging by the inhibition of cGAS-STING signaling due to YAP/TAZ mechanotransduction. , In addition, the nuclear shape is changed by cytoskeleton force to regulate chromatin remodeling and phenotype alternation. − Therefore, in this study, the nuclear DNA synthesis level could be regulated by the mechanotransduction from anisotropic YAP signaling and biased cell nanomechanics due to chiral FA formation and heterogeneous cytoskeleton assembly (Figure ).…”

Section: Discussionmentioning

confidence: 84%

Chiral Cell Nanomechanics Originated in Clockwise/Counterclockwise Biofunctional Microarrays to Govern the Nuclear Mechanotransduction of Mesenchymal Stem Cells

Wang,

Tong,

Shi

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Cell chirality is extremely important for the evolution of cell morphogenesis to manipulate cell performance due to left−right asymmetry. Although chiral micro-and nanoscale biomaterials have been developed to regulate cell functions, how cell chirality affects cell nanomechanics to command nuclear mechanotransduction was ambiguous. In this study, chiral engineered microcircle arrays were prepared by photosensitive crosslinking synthesis on cell culture plates to control the clockwise/counterclockwise geometric topology of stem cells. Asymmetric focal adhesion and cytoskeleton structures could induce chiral cell nanomechanics measured by atomic force microscopy (AFM) nanoindentation in left-/right-handed stem cells. Cell nanomechanics could be enhanced when the construction of mature focal adhesion and the assembly of actin and myosin cytoskeletons were well organized in chiral engineered stem cells. Curvature angles had a negative effect on cell nanomechanics, while cell chirality did not change cytoskeletal mechanics. The biased cytoskeleton tension would engender different nuclear mechanotransductions by yes-associated protein (YAP) evaluation. The chiral stimuli were delivered into the nuclei to oversee nuclear behaviors. A strong cell modulus could activate high nuclear DNA synthesis activity by mechanotransduction. The results will bring the possibility of understanding the interplay of chiral cell nanomechanics and mechanotransduction in nanomedicines and biomaterials.

show abstract

Section: Discussionmentioning

confidence: 84%

Chiral Cell Nanomechanics Originated in Clockwise/Counterclockwise Biofunctional Microarrays to Govern the Nuclear Mechanotransduction of Mesenchymal Stem Cells

Wang,

Tong,

Shi

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, a fusion protein of IL-2 and pHLIP (IL2-pHLIP) demonstrated tumor targeting and resulted in effective reduction of breast and melanoma tumors in animal tumor models ( Chu et al, 2023 ). Another example of targeted delivery of chemokines is a fusion of chemokine C-C motif ligand 21 (CCL21) with pHLIP ( Li et al, 2023 ). CCL21 binds to the CCR7 cell-surface chemokine receptor found on leukocytes ( Yoshida et al, 1998 ).…”

Section: Extracellular Delivery Of Imaging and Therapeutic Payloads B...mentioning

confidence: 99%

Aiming the magic bullet: targeted delivery of imaging and therapeutic agents to solid tumors by pHLIP peptides

Reshetnyak,

Andreev,

Engelman

2024

Front. Pharmacol.

View full text Add to dashboard Cite

The family of pH (Low) Insertion Peptides (pHLIP) comprises a tumor-agnostic technology that uses the low pH (or high acidity) at the surfaces of cells within the tumor microenvironment (TME) as a targeted biomarker. pHLIPs can be used for extracellular and intracellular delivery of a variety of imaging and therapeutic payloads. Unlike therapeutic delivery targeted to specific receptors on the surfaces of particular cells, pHLIP targets cancer, stromal and some immune cells all at once. Since the TME exhibits complex cellular crosstalk interactions, simultaneous targeting and delivery to different cell types leads to a significant synergistic effect for many agents. pHLIPs can also be positioned on the surfaces of various nanoparticles (NPs) for the targeted intracellular delivery of encapsulated payloads. The pHLIP technology is currently advancing in pre-clinical and clinical applications for tumor imaging and treatment.

show abstract

Encapsulation of individual mammalian cells as a cell-based drug delivery carrier for lung cancer treatment

Shi,

Chen,

et al. 2025

Journal of Controlled Release

View full text Add to dashboard Cite

Display of CCL21 on cancer cell membrane through genetic modification using a pH low insertion peptide

Cited by 4 publications

References 32 publications

Chiral Cell Nanomechanics Originated in Clockwise/Counterclockwise Biofunctional Microarrays to Govern the Nuclear Mechanotransduction of Mesenchymal Stem Cells

Chiral Cell Nanomechanics Originated in Clockwise/Counterclockwise Biofunctional Microarrays to Govern the Nuclear Mechanotransduction of Mesenchymal Stem Cells

Aiming the magic bullet: targeted delivery of imaging and therapeutic agents to solid tumors by pHLIP peptides

Encapsulation of individual mammalian cells as a cell-based drug delivery carrier for lung cancer treatment

Contact Info

Product

Resources

About