Disposable carbon nanotube scaffold films for fast and reliable assessment of total α1-acid glycoprotein in human serum using adsorptive transfer stripping square wave voltammetry

Sierra, Tania; Dortez, Silvia; González, Marı́a Cristina; Palomares, F. J.; Crevillén, Agustín G.; Escarpa, Alberto

doi:10.1007/s00216-018-1419-6

Cited by 6 publications

(8 citation statements)

References 48 publications

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“…These kinds of nanomaterials (SWCNT and MWCNT) showed excellent analytical performance for electrochemical detection of glycoproteins. 21 For this reason, we explored other types of carbon nanomaterials (DWCNT). Figure 3A shows the square wave voltammograms corresponding to the different electrodes using 1 mM FcTMA + as the redox probe.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…This result agrees with previous works on the characterization of this tag. 20,21 The DWE showed the best analytical response, and the TPE showed the poorest performance.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…However, the protocol to label the glycoprotein was too long (around 16 h), which complicates its implementation as POCT. In the second example, 21 they took the advantage of the favorable kinetics of a chemical approach based on an exchange of ligands to achieve high stability of the products [AGP-Os(VI)TEMED], allowing a dramatic reduction in labeling time to 12 min (offchip).…”

Section: T H Imentioning

confidence: 99%

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Pump-Free Microfluidic Device for the Electrochemical Detection of α₁-Acid Glycoprotein

et al. 2021

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α1-Acid glycoprotein (AGP) is a glycoprotein present in serum, which is associated with the modulation of the immune system in response to stress or injuries, and a biomarker for inflammatory diseases and cancers. Here, we propose a pump-free microfluidic device for the electrochemical determination of AGP. The microfluidic device utilizes capillary-driven flow and a passive mixing system to label the AGP with the Os (VI) complex (an electrochemical tag) inside the main channel, before delivering the products to the electrode surface. Furthermore, thanks to the resulting geometry, all the analytical steps can be carried out inside the device: labeling, washing, and detection by adsorptive transfer stripping square wave voltammetry. The microfluidic device exhibited a linear range from 500 to 2000 mg L–1 (R 2 = 0.990) and adequate limit of detection (LOD = 231 mg L–1). Commercial serum samples were analyzed to demonstrate the success of the method, yielding recoveries around 83%. Due to its simplicity, low sample consumption, low cost, short analysis time, disposability, and portability, the proposed method can serve as a point-of-care/need testing device for AGP.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…This result agrees with previous works on the characterization of this tag. 20,21 The DWE showed the best analytical response, and the TPE showed the poorest performance.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: T H Imentioning

confidence: 99%

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Pump-Free Microfluidic Device for the Electrochemical Detection of α₁-Acid Glycoprotein

et al. 2021

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“…In this sense, Palecek’s group published several works in which glycoproteins were detected using an osmium(VI) complex as electrochemical probe. , They demonstrated that this Os(VI) complex specifically links to the carbohydrate part and not to the polypeptide backbone. Specifically, the Os(VI) complexes react with diol groups from saccharides and produce osmate esters, which generate two electrochemical signals in carbon and mercury electrodes at −0.70 and −0.25 V. , Recently, our group has demonstrated the usefulness of this Os(VI) complex in the off-chip determination of AGP in serum samples. , …”

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confidence: 99%

“…Specifically, the Os(VI) complexes react with diol groups from saccharides and produce osmate esters, which generate two electrochemical signals in carbon and mercury electrodes at −0.70 and −0.25 V. 36,37 Recently, our group has demonstrated the usefulness of this Os(VI) complex in the off-chip determination of AGP in serum samples. 38,39 In the following sections, we will demonstrate for the first time the analysis of glycoproteins by microchip electrophoresis with amperometric detection (ME-ED) using Os-based electrochemistry, under an oxidation potential so far completely unexplored in the literature.…”

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confidence: 99%

Determination of Glycoproteins by Microchip Electrophoresis Using Os(VI)-Based Selective Electrochemical Tag

2019

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Glycoproteins are excellent biomarkers for diagnosis and prognosis of several illnesses. α-1-Acid glycoprotein (AGP) and transferrin (Tf) are the main glycoproteins present in the serum, whose levels are increased during disease or injury. In this work, a selective detection of these glycoproteins using a microchip electrophoresis-electrochemical detection (ME-ED) platform is proposed for the first time. Because of the reduced sensitivity of glycoproteins, they were labeled with an electrochemical tag (osmium(VI) complex), which binds only to glycans, increasing the amperometric signal. Interestingly, oxidation potential of glycoprotein-Os(VI) adducts started at +0.50 V (vs Ag/AgCl) while nontagged glycoproteins started at +0.60 V. So, when the detection potential is set at +0.50 V, only glycoproteins tagged with Os(VI) complex are detected, avoiding the interference of the rest of the proteins. Determination of AGP and Tf was successfully demonstrated in the analysis of a certified human serum reference material yielding excellent accuracy (E r ≤ 4%) in just 400 s. This work offers new possibilities for ED for glycoprotein analysis in microfluidic systems, which has been dominated by fluorescence and MS detection until now. It is worth mentioning that ED has two interesting advantages with respect to others in the point-of-care testing field: easy miniaturization (bedside devices) and low cost.

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Electropolymerization of thionine as a stable film along with carbon nanotube for sensitive detection of tetracycline antibiotic drug

et al. 2020

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Disposable carbon nanotube scaffold films for fast and reliable assessment of total α1-acid glycoprotein in human serum using adsorptive transfer stripping square wave voltammetry

Cited by 6 publications

References 48 publications

Pump-Free Microfluidic Device for the Electrochemical Detection of α₁-Acid Glycoprotein

Pump-Free Microfluidic Device for the Electrochemical Detection of α₁-Acid Glycoprotein

Determination of Glycoproteins by Microchip Electrophoresis Using Os(VI)-Based Selective Electrochemical Tag

Electropolymerization of thionine as a stable film along with carbon nanotube for sensitive detection of tetracycline antibiotic drug

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Disposable carbon nanotube scaffold films for fast and reliable assessment of total α1-acid glycoprotein in human serum using adsorptive transfer stripping square wave voltammetry

Cited by 6 publications

References 48 publications

Pump-Free Microfluidic Device for the Electrochemical Detection of α1-Acid Glycoprotein

Pump-Free Microfluidic Device for the Electrochemical Detection of α1-Acid Glycoprotein

Determination of Glycoproteins by Microchip Electrophoresis Using Os(VI)-Based Selective Electrochemical Tag

Electropolymerization of thionine as a stable film along with carbon nanotube for sensitive detection of tetracycline antibiotic drug

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Product

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Pump-Free Microfluidic Device for the Electrochemical Detection of α₁-Acid Glycoprotein

Pump-Free Microfluidic Device for the Electrochemical Detection of α₁-Acid Glycoprotein