Disposable label-free electrochemical immunosensor based on prussian blue nanocubes for four breast cancer tumor markers

Kongkaew, Supatinee; Cotchim, Suparat; Kanatharana, Proespichaya; Thavarungkul, Panote; Limbut, Warakorn

doi:10.1016/j.talanta.2022.124229

Cited by 26 publications

(5 citation statements)

References 41 publications

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“…The detection limit (DL) calculated based on a signal-to-noise ratio (S/N) of 3 was 0.13 mU/mL. Table 1 summarized the comparison of the analytical performance obtained on different modified electrodes for the detection of CA15-3 using electroluminescence (ECL), photoelectrochemical (PEC) or electrochemical (EC) methods ( Cui et al, 2014 ; Bao et al, 2021 ; Zhong et al, 2021 ; Kongkaew et al, 2023 ; Lin et al, 2023 ). As seen, the electrochemical aptasensor established in this paper has a wide linear range and a low LOD.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical aptasensor fabricated by anchoring recognition aptamers and immobilizing redox probes on bipolar silica nanochannel array for reagentless detection of carbohydrate antigen 15-3

Xing,

Han,

Liu

et al. 2023

Front. Chem.

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Timely, convenient, and efficient detection of carbohydrate antigen 15-3 (CA15-3) levels in serum holds significant importance in early screening, diagnostic assistance and prognosis prediction of breast cancer. The development of efficient and convenient electrochemical aptasensors with immobilized redox probes for label-free detection of CA15-3 is highly desirable. In this work, a bipolar silica nanochannel array film (bp-SNA) with two distinct functional domains including nanochannels and an outer surface was employed for the immobilization of recognition ligands and electrochemical redox probes, enabling the construction of a probe-integrated aptasensor for reagentless electrochemical detection of CA15-3. Cost-effective and readily available indium tin oxide (ITO) was used as the supporting electrode for sequential growth of a negatively charged inner layer (n-SNA) followed by a positively charged outer layer (p-SNA). The preparation process of bp-SNA is convenient. Functionalization of amino groups on the outer surface of bp-SNA was modified by aldehyde groups for covalent immobilization of recognition aptamers, further establishing the recognition interface. Within the nanochannels of bp-SNA, the electrochemical redox probe, tri (2,2′-dipyridyl) cobalt (II) (Co(bpy)32+) was immobilized, which experienced a dual effect of electrostatic attraction from n-SNA and electrostatic repulsion from p-SNA, resulting in high stability of the immobilized probes. The constructed aptasensor allowed for reagentless electrochemical detection of CA15-3 ranged from 0.001 U/mL to 500 U/mL with a low detection limit (DL), 0.13 mU/mL). The application of the constructed aptasensor for CA15-3 detection in fetal bovine serum was also validated. This sensor offers advantages of a simple and readily obtainable supporting electrode, easy bp-SNA fabrication, high probe stability and good stability.

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Section: Resultsmentioning

confidence: 99%

Electrochemical aptasensor fabricated by anchoring recognition aptamers and immobilizing redox probes on bipolar silica nanochannel array for reagentless detection of carbohydrate antigen 15-3

Xing,

Han,

Liu

et al. 2023

Front. Chem.

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“…The accuracy of the results was confirmed by measuring the electrochemical properties of the reacted and unreacted PBNPs to achieve two complementary measurement modes of signal switching with LODs of 5.2 μU mL −1 and 0.41 μU mL −1 , respectively. Kongkaew et al [82] proposed a multi-electrode array consisting of four working electrodes, shared reference electrodes, and auxiliary electrodes for the detection of four breast cancer tumor markers (CEA, CA125, CA153, and CA199). In addition, Zhu et al [83] developed an isolated photoimmunoassay for CEA using polydopamine-encapsulated PBNPs as a signal marker.…”

Section: Electrochemical Immunoassaysmentioning

confidence: 99%

Advances in nanoprobes‐based immunoassays

Zhang,

Huang,

Jin

et al. 2023

BMEMat

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Immunoassay is a powerful technique that uses highly specific antigen‐antibody interactions to detect biochemical targets such as proteins and toxins. As a diagnostic tool, immunoassay is employed in the screening, diagnosis, and prognosis of diseases, which are crucial for the grasp and control of patient conditions in clinical practice. With the rapid development of nanotechnology, immunoassays based on nanoprobes have attracted more and more attention due to the advantages of high sensitivity, specificity, stability, and versatility. These nanoprobes are nanoscale particles that can act as signal carriers or targeting agents for immunoassays. In this paper, we review the recent advances in various types of nanoprobes for immunoassays, such as colloidal gold, quantum dots, magnetic nanoparticles, nanozymes, aggregation‐induced emission, and up‐conversion nanoparticles. The effect of the nanoprobe construction and synthesis methods on their detection performance deserves to be studied in depth. We also compare their detection ranges and limits in different immunoassay methods, such as lateral flow immunoassays, fluorescent immunoassays, and surface‐enhanced Raman scattering immunoassays. Moreover, we discuss the benefits and challenges of nanoprobes in immunoassays and provide insights into their future development. This study aims to offer a comprehensive and critical perspective on the role of nanoprobes in the field of immunoassays.

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“…Kongkaew S. et al [ 48 ] report that the multi-electrode array (MEA) was fabricated in the presence of working, reference, and auxiliary electrodes. Prussian blue nanocubes (PBNCs) were synthesized and applied by cyclic voltammetry.…”

Section: Application Of Sensorsmentioning

confidence: 99%

Comparative study of electrochemical-based sensors and immunosensors in terms of advantageous features for detection of cancer biomarkers

ÖZÇELİKAY,

ÇETİNKAYA,

KAYA

et al. 2023

Turkish Journal of Chemistry

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Cancer, becoming increasingly common globally, has a high mortality rate. Despite the much research on diagnosis and treatment methods, the benefits of technological developments, and newly developed sensor devices, cancer is still one of the leading causes of death worldwide. Early detection using powerful and noninvasive tools could be a future focus for prognosis and treatment follow-up. Therefore, electrochemical biosensors can be a strong choice for the detection of cancer biomarkers (such as alpha-fetoprotein, cytochrome c, prostate-specific antigen, myoglobin, carcinoembryonic antigen, alpha-fetoprotein, a cancer antigen, epidermal growth factor receptor, vascular endothelial growth factor, circulating tumor cell, and breast cancer antigen 1/2) due to their advantages such as high sensitivity, excellent selectivity, low cost, short analysis time, and simplicity. Furthermore, electrochemical biosensors are better suited for point-of-care applications due to their mass production and miniaturization ease. This review provides an overview of different electrochemical measurement techniques, bioreceptor surfaces, signal production and amplification, and the integration of electrochemical-modified sensors. Cancer biomarkers based on electrochemical biosensors were given in detail. In addition, studies with MIP-based sensors and immunosensors have been extensively discussed. Integrating electrochemical biosensors with cancer biomarkers was also emphasized as a new research trend. Finally, we provide an overview of current advances in measuring and analyzing cancer biomarkers using electrochemical biosensors and detail current challenges and future perspectives.

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Disposable label-free electrochemical immunosensor based on prussian blue nanocubes for four breast cancer tumor markers

Cited by 26 publications

References 41 publications

Electrochemical aptasensor fabricated by anchoring recognition aptamers and immobilizing redox probes on bipolar silica nanochannel array for reagentless detection of carbohydrate antigen 15-3

Electrochemical aptasensor fabricated by anchoring recognition aptamers and immobilizing redox probes on bipolar silica nanochannel array for reagentless detection of carbohydrate antigen 15-3

Advances in nanoprobes‐based immunoassays

Comparative study of electrochemical-based sensors and immunosensors in terms of advantageous features for detection of cancer biomarkers

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