Disposition kinetics and urinary excretion of cefpirome after intravenous injection in buffalo calves

Abstract

We investigated the disposition kinetics and urinary excretion of cefpirome in buffalo calves after a single intravenous administration of 10 mg/kg. Also, an appropriate dosage regimen was calculated. At 1 min after injection, the concentration of cefpirome in the plasma was 57.4 ± 0.72 µg/ml, which declined to 0.22 ± 0.01 µg/ml at 24 h. The cefpirome was rapidly distributed from the blood to the tissue compartment as shown by the high distribution coefficient values (8.67 ± 0.46/h), and by the drug's rate of transfer constant from the central to the peripheral compartment, K12 (4.94 ± 0.31/h). The elimination halflife and the volume of distribution were 2.14 ± 0.02 h and 0.42 ± 0.005 l/kg, respectively. Once the distribution equilibrium was reached between the tissues and plasma, the total body clearance (ClB) and the ratio of the drug present in the peripheral to the central compartment (T/P ratio) were 0.14 ± 0.002 l/kg/h and 1.73 ± 0.06, respectively. Based on the pharmacokinetic parameters we obtained, an appropriate intravenous cefpirome dosage regimen for treating cefpiromesensitive bacteria in buffalo calves would be 8.0 mg/kg repeated at 12 h intervals for 5 days, or until persistence of the bacterial infection occurred.

Development and Validation of Uv Spectrophotometry and Liquid Chromatography Methods for Determination of Cefpirome in Raw Material and Pharmaceutical Dosage

“…Methods have been reported for the determination of cefpirome in plasma, serum, urine, tissue, raw material and pharmaceutical form. These methods include microbiological assay, HPLC, voltammetry assay, UV spectrometry and pH potentiometry (2,(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). The aim of this study was to develop and validate simple and rapid UV Spectrophotometry and Liquid Chromatography (HPLC) methods for the quantification of cefpirome in raw material and powder for injectable preparation.…”

Microbiological Assay for the Determination of Cefpirome in Raw Material and Injectable Preparation

“…The increased spectrum of activity, together with high stability against -lactamases and rapid transmembrane transport, distinguishes cefpirome as an example of a fourth-generation cephalosporin and its principal use is in treatment for patients' septic shock or several sepsis [2,[4][5][6][7][8][9]. Methods have been reported for the determination of cefpirome biological fluids [2,[6][7][8][9][10][11][12] and pharmaceutical dosage forms [13][14][15]. Until now, there isn't any report for the determination of cefpirome in raw material and pharmaceutical preparation by biossay.…”