Disrupted gonadogenesis and male-to-female sex reversal in<i>Pod1</i>knockout mice

Cui, Shiying; Ross, Andrea J.; Stallings, Nancy R.; Parker, Keith L.; Capel, Blanche; Quaggin, Susan E.

doi:10.1242/dev.01266

Cited by 162 publications

(166 citation statements)

References 45 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…At later stages Pod1 expression becomes sexually dimorphic and it is found in the testis interstitium but not within the ovary [65]. Pod1 was shown to repress Sf-1 expression in tissue culture experiments [65][66][67]. Consistent with this, Pod1 deficient embryos showed an expanded Sf-1 expression domain at the boundary between the gonad and mesonephros.…”

Section: Page 7 Of 23mentioning

confidence: 63%

“…Although Pod1 was not directly involved in adrenal development, its disruption prevented the physical separation between the gonad and adrenal primordia. Interestingly, there was no increase in adrenal markers expression in the gonad which suggested that differentiation and sorting of the precursors were not affected [66]. Thus Pod1 acts to restrict Sf-1 expression such that the adrenal and gonad primordia are separated during development.…”

Section: Page 7 Of 23mentioning

confidence: 96%

See 1 more Smart Citation

Gene dosage effects and transcriptional regulation of early mammalian adrenal cortex development

Val

Swain

2010

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

show abstract

Section: Page 7 Of 23mentioning

confidence: 63%

Section: Page 7 Of 23mentioning

confidence: 96%

Gene dosage effects and transcriptional regulation of early mammalian adrenal cortex development

Val

Swain

2010

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

show abstract

“…In Sf1 heterozygous embryos, Dhh expression was temporarily reduced at E11.5, and fetal Leydig cell markers Cyp17 and Cyp11a1 were reduced at E13.5 [58], suggesting an indirect role for SF1 in initial fetal Leydig differentiation via DHH. Additionally, a null mutation of Pod1, a helix-turn-helix transcription factor expressed in the gonadal interstitium, increased the number of steroidogenic cells in both male and female gonads but reduced androgen production [59]. These results indicate that fetal Leydig cell differentiation is controlled externally through pathways elicited by Sertoli cells and intrinsically by transcription factors.…”

Section: From Sry To Androgensmentioning

confidence: 86%

The road to maleness: from testis to Wolffian duct

Barsoum

Yao

2006

Trends in Endocrinology & Metabolism

View full text Add to dashboard Cite

The establishment of the male internal reproductive system involves two crucial events: the formation of the testis and the maintenance and differentiation of the Wolffian duct. Testis formation, particularly the specification of Sertoli cell and Leydig cell lineages, is controlled strictly by genetic components initiated by the testis-determining gene SRY (sex-determining region of the Y chromosome). Conversely, Wolffian duct differentiation is not directly mediated via the composition of the sex chromosome or SRY; instead, it relies on androgens derived from the Leydig cells. Leydig cells do not express SRY, indicating that a crosstalk must be present between the SRY-positive Sertoli and Leydig cells to ensure normal androgen production. Recent advancement of genetic and genomic approaches has unveiled the molecular pathways for differentiation of Sertoli cells and Leydig cells as well as development of the Wolffian duct.

show abstract

“…and fixed in 4% PFA. Ten-micrometer cryosections were prepared as described previously (16). The primary antibodies used were anti-␣ 8 integrin (1:1500 dilution).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…The immunofluorescent staining procedure was described previously (16). The secondary antibodies used were FITC-conjugated goat anti-rabbit IgG (Jackson Laboratories, West Grove, PA; 1:400) to detect anti-␣ 8 integrin and Cy3-conjugated donkey anti-mouse IgG (Jackson Laboratories, 1:500) to detect anti-lacZ.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Rapid Isolation of Glomeruli Coupled with Gene Expression Profiling Identifies Downstream Targets in Pod1 Knockout Mice

Cui

Ema

et al. 2005

Journal of the American Society of Nephrology

Self Cite

View full text Add to dashboard Cite

Mouse mutations have provided tremendous insights into the molecular basis of renal and glomerular development. However, genes often play important roles during multiple stages of nephrogenesis, making it difficult to determine the role of a gene in a specific cell lineage such as the podocyte. Conditional gene targeting and chimeric analysis are two possible approaches to dissect the function of genes in specific cell populations. However, these are labor-intensive and costly and require the generation, validation, and analysis of additional transgenic lines. For overcoming these shortcomings and, specifically, for studying the role of gene function in developing glomeruli, a technique to isolate and purify glomeruli from murine embryos was developed. Combined with gene expression profiling, this method was used to identify differentially expressed genes in glomeruli from Pod1 knockout (KO) mice that die in the perinatal period with multiple renal defects. Glomeruli from early developing stages (late S-shape/early capillary loop) onward can be isolated successfully from wild-type and KO kidneys at 18.5 d postcoitus, and RNA can readily be obtained and used for genome-wide microarray analysis. With this approach, 3986 genes that are differently expressed between glomeruli from Pod1 KO and wild-type mice were identified, including a four-fold reduction of ␣ 8 integrin mRNA in glomeruli from Pod1 KO mice that was confirmed by immunostaining. This procedure may be adapted to any transgenic strain, providing a rapid and efficient method to dissect the function of specific genes in glomerular development.

show abstract

Disrupted gonadogenesis and male-to-female sex reversal inPod1knockout mice

Cited by 162 publications

References 45 publications

Gene dosage effects and transcriptional regulation of early mammalian adrenal cortex development

Gene dosage effects and transcriptional regulation of early mammalian adrenal cortex development

The road to maleness: from testis to Wolffian duct

Rapid Isolation of Glomeruli Coupled with Gene Expression Profiling Identifies Downstream Targets in Pod1 Knockout Mice

Contact Info

Product

Resources

About