Disruption and overexpression of 6-phosphofructo-2-kinase influence organic acid production in Aspergillus carbonarius ITEM 5010

Yang, Lei; Lübeck, Mette; Ahring, Birgitte Kiær; Bruno, Kenneth S.; Lübeck, Peter Stephensen

doi:10.1007/s11274-020-02877-4

Cited by 1 publication

(2 citation statements)

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“…Previous studies have shown that intracellular stress, mainly induced by nitrogen limitation during fermentation, is one of the primary requirements for stimulating filamentous fungi to produce and accumulate organic acids (Knuf et al, 2013; Ochsenreither et al, 2014; Sebastian et al, 2019). Under stress conditions, filamentous fungi convert carbon sources such as glucose into organic acids through glycolysis and other pathways (L. Yang et al, 2020; X. Yin et al, 2015). However, stress affects the physiological metabolic activities of filamentous fungi to a certain extent, thereby deteriorating fermentation performance.…”

Section: Introductionmentioning

confidence: 99%

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Peptide supplementation relieves stress and enhances glycolytic flux in filamentous fungi during organic acid bioproduction

Zhang

et al. 2022

Biotech & Bioengineering

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Filamentous fungi occupy a uniquely favorable position in the bioproduction of organic acids. Intracellular stress is the main stimulator in filamentous fungi to produce and accumulate organic acids with high flux. However, stress can affect the physiological activities of filamentous fungi, thereby deteriorating their fermentation performance. Herein, we report that peptide supplementation during Rhizopus oryzae fermentation significantly improved fumaric acid production. Specifically, fumaric acid productivity was elevated by approximately 100%, fermentation duration was shortened from 72 to 36 h, while maintaining the final titer. Furthermore, transcriptome profile analysis and biochemical assays indicated that the overall capabilities of the stress defense systems (enzymatic and nonenzymatic) were significantly improved in R. oryzae. Consequently, glycolytic metabolism was distinctly enhanced, which eventually resulted in improved fumaric acid production and reduced fermentation duration. We expect our findings and efforts to provide essential insights into the optimization of the fermentation performance of filamentous fungi in industrial biotechnology and fermentation engineering.

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Section: Introductionmentioning

confidence: 99%

“…primary requirements for stimulating filamentous fungi to produce and accumulate organic acids (Knuf et al, 2013;Ochsenreither et al, 2014;Sebastian et al, 2019). Under stress conditions, filamentous fungi convert carbon sources such as glucose into organic acids through glycolysis and other pathways (L. Yang et al, 2020;X. Yin et al, 2015).…”

mentioning

confidence: 99%

Peptide supplementation relieves stress and enhances glycolytic flux in filamentous fungi during organic acid bioproduction

Zhang

et al. 2022

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

Disruption and overexpression of 6-phosphofructo-2-kinase influence organic acid production in Aspergillus carbonarius ITEM 5010

Cited by 1 publication

References 21 publications

Peptide supplementation relieves stress and enhances glycolytic flux in filamentous fungi during organic acid bioproduction

Peptide supplementation relieves stress and enhances glycolytic flux in filamentous fungi during organic acid bioproduction

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