Disruption of a phytochrome-like histidine kinase gene by homologous recombination leads to a significant reduction in vegetative growth, sclerotia production, and the pathogenicity of Botrytis cinerea

Hu, Youzhen; He, Jian‐Qing; Wang, Yiwen; Zhu, Pinkuan; Zhang, Chenghua; Lu, Rensen; Xu, Lingfei

doi:10.1016/j.pmpp.2013.12.002

Cited by 17 publications

(19 citation statements)

References 39 publications

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“…Phylogenetic analysis indicates that Bbphy shares 34–60% sequence identity with the Phy homologues in four other fungi and is more closely related to bacterial homologues than those in plants (Supporting Information http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12486/suppinfo). However, Bbphy lacks a transmembrane domain like the Phy homologues found in A. nidulans (Blumenstein et al ., ), N. crassa (Froehlich et al ., ) and B. cinerea (Hu et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…C), than parental WT and Δ Bbphy/Bbphy (control strains) ( P < 0.001 in F tests) during 7‐day incubation at 25°C under the white light/dark cycles of 3:21 to 24:0 h. However, the tested cycles did not significantly affect the radial growth of any fungal strain in either SDAY, a standard medium for cultivation of fungal entomopathogens, or CZA ( P ≥ 0.10 in F tests). The slower growth of Δ Bbphy in SDAY was similar to a situation of B. cinerea Δ Bcphy3 grown in potato dextrose agar under an undisclosed photoperiod (Hu et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…As presented above, Bbphy, a Group VIII HK in B. bassiana , is characteristic with an N‐terminal phytochrome domain for light sensing (Purschwitz et al ., ) and lacks a transmembrane domain like other fungal Phy homologues (Blumenstein et al ., ; Froehlich et al ., ; Hu et al ., ). Bbphy is functionally more diverse than the counterparts of other fungi characterized in previous studies, as discussed below.…”

Section: Discussionmentioning

confidence: 97%

“…Aside from the remarkable effect on asexual development, Bbphy may participate not only in the osmoregulation of B. bassiana but in the regulation of antioxidant response, conidial thermotolerance and UV‐B resistance. The null response of Δ Bbphy to cell wall perturbation is different from what was reported from B. cinerea Δ Bcphy3 , which was more sensitive to Congo red during colony growth (Hu et al ., ). The increased tolerance of our deletion mutant to oxidative stress is in agreement with the upregulated transcripts of all five SOD genes and four CAT genes.…”

Section: Discussionmentioning

confidence: 97%

“…In addition, our bioassay data implicate that Bbphy could not be involved in the B. bassiana response to host cues generated during cuticle penetration or after entry into hemocoel and hence contributed little to the fungal virulence. This is very different from a significant contribution of Bcphy3 to the pathogenicity of B. cinerea (Hu et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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Phytochrome controls conidiation in response to red/far‐red light and daylight length and regulates multistress tolerance in Beauveria bassiana

Qiu

Wang

Chu

et al. 2014

Environmental Microbiology

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Phytochromes (Phy) in filamentous fungi are Group VIII histidine kinases that share a unique N-terminal photosensory core, but their functions are largely unknown. Here we show that Beauveria bassiana Phy (Bbphy) is functionally vital for growth, conidiation and multistress tolerance of the fungal entomopathogen lacking sexual stage. Colony growth of ΔBbphy was significantly slower in a nutrition-rich medium but faster in several minimal media. Conidial yield of ΔBbphy in the rich medium increased at the fitted rate of 3.4 × 10(7) conidia h(-1) white light in the light/dark cycles of 0:24 to 16:8 h, decreased greatly in the short-, long- and full-day cycles of red/far-red light, but was unaffected under full-day blue light. Moreover, ΔBbphy showed higher osmosensitivity, increased antioxidant capability, and decreased conidial thermotolerance and UV-B resistance, accompanied with downregulation of Hog1 phosphorylation and of four Hog1-related genes under osmotic stress, and upregulation of five superoxide dismutases and four catalases under oxidative stress. All the changes were restored by the gene complementation. Taken together, Bbphy controls conidiation by responding to daylight length and red/far-red light and regulates multistress responses perhaps because of an involvement in Hog1 pathway. Our findings highlight diverse functions of Bbphy in B. bassiana.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

See 3 more Smart Citations

Phytochrome controls conidiation in response to red/far‐red light and daylight length and regulates multistress tolerance in Beauveria bassiana

Qiu

Wang

Chu

et al. 2014

Environmental Microbiology

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3 The Bright and Dark Sides of Fungal Life

Casas-Flores

Herrera‐Estrella²

2016

Environmental and Microbial Relationships

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A light life together: photosensing in the plant microbiota

Losi

Gärtner

2021

Photochem Photobiol Sci

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Bacteria and fungi of the plant microbiota can be phytopathogens, parasites or symbionts that establish mutually advantageous relationships with plants. They are often rich in photoreceptors for UVA–Visible light, and in many cases, they exhibit light regulation of growth patterns, infectivity or virulence, reproductive traits, and production of pigments and of metabolites. In addition to the light-driven effects, often demonstrated via the generation of photoreceptor gene knock-outs, microbial photoreceptors can exert effects also in the dark. Interestingly, some fungi switch their attitude towards plants in dependence of illumination or dark conditions in as much as they may be symbiotic or pathogenic. This review summarizes the current knowledge about the roles of light and photoreceptors in plant-associated bacteria and fungi aiming at the identification of common traits and general working ideas. Still, reports on light-driven infection of plants are often restricted to the description of macroscopically observable phenomena, whereas detailed information on the molecular level, e.g., protein–protein interaction during signal transduction or induction mechanisms of infectivity/virulence initiation remains sparse. As it becomes apparent from still only few molecular studies, photoreceptors, often from the red- and the blue light sensitive groups interact and mutually modulate their individual effects. The topic is of great relevance, even in economic terms, referring to plant-pathogen or plant-symbionts interactions, considering the increasing usage of artificial illumination in greenhouses, the possible light-regulation of the synthesis of plant-growth stimulating substances or herbicides by certain symbionts, and the biocontrol of pests by selected fungi and bacteria in a sustainable agriculture.

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Disruption of a phytochrome-like histidine kinase gene by homologous recombination leads to a significant reduction in vegetative growth, sclerotia production, and the pathogenicity of Botrytis cinerea

Cited by 17 publications

References 39 publications

Phytochrome controls conidiation in response to red/far‐red light and daylight length and regulates multistress tolerance in Beauveria bassiana

Phytochrome controls conidiation in response to red/far‐red light and daylight length and regulates multistress tolerance in Beauveria bassiana

3 The Bright and Dark Sides of Fungal Life

A light life together: photosensing in the plant microbiota

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