Disruption of Angiogenesis by Anthocyanin-Rich Extracts of Hibiscus sabdariffa

Abstract

Abnormal vessel formations contribute to the progression of specific angiogenic diseases including age-related macular degeneration. Adequate vessel growth and maintenance represent the coordinated process of endothelial cell proliferation, matrix remodeling, and differentiation. However, the molecular mechanism of the proper balance between angiogenic activators and inhibitors remains elusive. In addition, quantitative analysis of vessel formation has been challenging due to complex angiogenic morphology. We hypothesized that conjugated double bond containing-natural products, including anthocyanin extracts from Hibiscus sabdariffa, may control the proper angiogenesis. The current study was designed to determine whether natural molecules from African plant library modulate angiogenesis. Further, we questioned how the proper balance of anti- or pro-angiogenic signaling can be obtained in the vascular microenvironment by treating anthocyanin or fatty acids using chick chorioallantoic membrane angiogenesis model in ovo. The angiogenic morphology was analyzed systematically by measuring twenty one angiogenic indexes using Angiogenic Analyzer software. Chick chorioallantoic model demonstrated that anthocyanin-rich extracts inhibited angiogenesis in time- and concentration-dependent manner. Molecular modeling analysis proposed that hibiscetin as a component in Hibiscus may bind to the active site of vascular endothelial growth factor receptor 2 (VEGFR2) with ΔG= −8.42 kcal/mol of binding energy. Our results provided the evidence that anthocyanin is an angiogenic modulator that can be used to treat uncontrolled neovascular-related diseases, including age-related macular degeneration.

Phyto-chemistry and Pharmacological Activities of Hibiscus sabdariffa Linn.-A Review

“…The investigation for the anticancer potential of the Hibiscus sabdariffa plant began roughly in the last decade that emerged with encouraging results [63][64][65][66][67][68][69]. Both crude extracts and pure compounds of the plant were reported to induce chemoprevention, selective cytotoxicity, cell cycle arrest, apoptosis, autophagy and antimetastatis effects in various types of human cancer cells.…”

“…Several researchers reported the cytotoxic, antimutagenic, antimetastatic, antiproliferative, apoptotic antiangiogenic activity of Hibiscus sabdariffa both in human and animal cancer cells. This activity of Hibiscus sabdariffa is due to the presence of polyphenols in it, which attributes towards its antioxidant and anticarcinogenic activities [64][65][66][67][68][69]. The anticancer potential of Hibiscus sabdariffa leaves extracts (methanol, ethanol, ethyl acetate and chloroform) on hepatocellular carcinoma cell line Hep 3B was tested and methanolic extract showed a greater cytotoxic effect when compared with others [70].…”

Noninvasive temporal detection of early retinal vascular changes during diabetes

“…According to a study reported by Joshua et al 75 , angiogenic parameters like extremities, nodes, junctions, master junction, master segments, master segment length, meshes, total mesh area, number of segments, branches, number of isolates segments, total length, total branching length, total segments length, total branches length, total isolated branches length, branching interval, total segments length /branches, mesh index, master segments length /master segments and mean mesh size could be successfully used to study the inhibition of angiogenesis in a chick embryo using an Angiogenic Analyzer software. These parameters were all used to show 50-60% inhibition of blood vessel formation in the sample where the angiogenesis inhibitor was tested.…”

Pharmaceutical properties of sour tea (Hibiscus sabdariffa), Toward an ideal treatment for Hypertension

“…HS inhibits angiogenesis and, therefore, can be helpful in treating angiogenesis-related diseases, including hypertension. 37 Sour tea induces Pharmaceutical Sciences (Indexed in ISI and Scopus) https://ps.tbzmed.ac.ir apoptosis in human gastric carcinoma cells through the p38 MAPK/FasL cascade pathway and p53 phosphorylation. In other words, HS extract acts as an apoptosis inducer in human gastric carcinoma (AGS) cells, and these discoveries are promising perspectives in human gastric cancer treatment.…”