Disruption of Biofilm Formation and Quorum Sensing in Pathogenic Bacteria by Compounds from Zanthoxylum Gilletti (De Wild) P.G. Waterman

Ikome, Hermia Nalova; Tamfu, Alfred Ngenge; Abdou, Jean Pierre; Fouotsa, Hugues; Nangmo, Pamela Kemda; Lah, Fidèle Castro Weyepe; Tchinda, Alembert T.; Ceylan, Özgür; Frédérich, Michel; Nkengfack, Augustin Ephrem

doi:10.1007/s12010-023-04380-6

Cited by 11 publications

(7 citation statements)

References 47 publications

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“…QS mediates the generation, diffusion and reception of small signal molecules that trigger virulence, resistance genes, toxin production, non-tolerance to antibiotics, drug efflux pumps and extracellular polysaccharide synthesis which constitutes the biofilm barrier [ 5 , 6 ]. Investigating the QS effects of phytochemicals during can shape the development of drugs that target QS-signal and receptors which contributes to antibiotic resistance, motility and biofilm formation [ 7 , 8 ]. Biofilm must be considered synonymously with antibiotic resistance because of its proficiency in transferring resistance genes between bacterial species and colonies as well as its impermeability and insusceptibility to antibiotics as well as efflux pump systemic elimination of antibiotics [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of anti-quorum sensing and antibiofilm effects of secondary metabolites from Gambeya lacourtiana (De Wild) Aubr. & Pellegr against selected pathogens

Talla,

Tamfu,

Wakeu

et al. 2023

BMC Complement Med Ther

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Background Microbial infections cause serious health problems especially with the rising antibiotic resistance which accounts for about 700,000 human deaths annually. Antibiotics which target bacterial death encounter microbial resistance with time, hence, there is an urgent need for the search of antimicrobial substances which target disruption of virulence factors such as biofilm and quorum sensing (QS) with selective pressure on the pathogens so as to avoid resistance. Methods Natural products are suitable leads for antimicrobial drugs that can inhibit bacterial biofilms and QS. Twenty compounds isolated from the medicinal plant Gambeya lacourtiana were evaluated for their antibiofilm and anti-quorum sensing effects against selected pathogenic bacteria. Results Most of the compounds inhibited violacein production in Chromobacterium violaceum CV12472 and the most active compound, Epicatechin had 100% inhibition at MIC (Minimal Inhibitory Concentration) and was the only compound to inhibit violacein production at MIC/8 with percentage inhibition of 17.2 ± 0.9%. Since the bacteria C. violaceum produces violacein while growing, the inhibition of the production of this pigment reflects the inhibition of signal production. Equally, some compounds inhibited violacein production by C. violaceum CV026 in the midst of an externally supplied acylhomoserine lactone, indicating that they disrupted signal molecule reception. Most of the compounds exhibited biofilm inhibition on Staphyloccocus aureus, Escherichia coli and Candida albicans and it was observed that the Gram-positive bacteria biofilm was most susceptible. The triterpenoids bearing carboxylic acid group, the ceramide and epicatechin were the most active compounds compared to others. Conclusion Since some of the compounds disrupted QS mediated processes in bacteria, it indicates that this plant is a source of antibiotics drugs that can reduce microbial resistance.

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Section: Introductionmentioning

confidence: 99%

Evaluation of anti-quorum sensing and antibiofilm effects of secondary metabolites from Gambeya lacourtiana (De Wild) Aubr. & Pellegr against selected pathogens

Talla,

Tamfu,

Wakeu

et al. 2023

BMC Complement Med Ther

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“…The anti-biofilm effect of BHBANA ( 6 ) at MIC and sub-MIC concentrations (1, 1/2, 1/4, and 1/8 MIC) on test pathogens was evaluated using a microplate biofilm method. 69 One percent of overnight bacterial cultures was introduced into 200 μL of Tryptose-Soy broth (TSB) containing glucose (0.25%) with or without the test compound and incubated at 37 °C for 48 h. The wells of the plates were then carefully emptied and rinsed with distilled water to remove the planktonic microbial cells. The control wells contained only broth and bacterial cells.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of a new diarylhydrazone derivative and an evaluation of its in vitro biofilm inhibition and quorum sensing disruption along with a molecular docking study

Boudiba

Tamfu

Hanini

et al. 2023

Journal of Chemical Research

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Molecules that target quorum sensing and biofilm inhibition are useful antimicrobials. In this regard, a new diarylhydrazone was synthesized and characterized using infrared, high-resolution mass spectrometry and nuclear magnetic resonance experiments as N-[( E)-4-bromo-2,5-diheptyloxybenzylideneamino]-2,4-dinitroaniline (BHBANA). Minimal inhibitory concentrations (MICs) vary from 0.625 to 2.5 mg mL−1. This compound was screened in vitro for its inhibition of quorum sensing–mediated violacein production by Chromobacterium violaceum CV12472 at MIC and sub-MIC and showed percentage inhibition varying from 100% at MIC to 5.7% ± 0.2% at MIC/32. Against Chromobacterium violaceum CV026, BHBANA exhibited anti-quorum-sensing zone diameters of 10.5 ± 0.3 mm and 7.0 ± 0.1 mm at MIC and MIC/2, respectively. BHBANA shows concentration-dependent inhibition of swarming motility on flagellated Pseudomonas aeruginosa PA01 with the highest % inhibition of 28.30% ± 0.50% μg mL−1 at MIC. The product inhibits biofilm formation, with the best biofilm inhibition being observed against Staphylococcus aureus varying from 72.24% ± 0.86% (MIC) to 09.82% ± 0.10% (MIC/8). Molecular docking studies carried out utilizing the Schrodinger software identified interactions between BHBANA and different receptor compartments of Chromobacterium violaceum, which can block pathogenic gene expression. The results suggest the potential of BHBANA in reducing microbial virulence.

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“…[9][10][11] The QS network in P. aeruginosa is therefore dependent on the density of the bacterial population and their ability to communicate with each other and it relies on the production and reception of small diffusible signal molecules mostly N-acyl-homoserine lactone (AHL) molecules. [12][13][14] When these diffusible pheromones reach a critical threshold concentration, they bind to the type "R" transcriptional regulator. The successful linkage of autoinducers to transcriptional regulators activates the expression of "I" target genes associated with pathogenicity, ecological adaptation, control of virulence factors, the formation of biofilms and the development of antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Disruption and Virulence Attenuation Effects of Essential Oil From Endemic Algerian Cistus munbyi (Cistaceae) Against Clinical Strains of Pseudomonas aeruginosa

Benaissa,

Khadir,

Tamfu

et al. 2024

Natural Product Communications

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Background: Pseudomonas aeruginosa is an opportunistic, resistant and highly pathogenic bacteria, responsible for severe nosocomial infections in humans. There is need to search new antimicrobials to target P. aeruginosa strains. Cistus munbyi is used in traditional medicine to manage infections. Objectives: This work aims to investigate the antimicrobial, antibiofilm and other antivirulence effects of essential oil from C. munbyi against P. aeruginosa. Materials and methods: Essential oil (EO) was prepared by hydro-distillation from C. munbyi, characterized by GC–MS and evaluated for its antimicrobial, antibiofilm and anti-virulence effects against clinical isolates of P. aeruginosa. Results: 100% of compounds were identified with Terpinen-4-ol (33.20%), Sabinene (13.20%), α-Thujene (11.30%), and p-Cymene (9.94%) being the major constituents. The EO had good antimicrobial activity determined by diameters of inhibition zones and broth dilution. At minimal inhibitory concentration (MIC), the EO eliminated more that 70% of young biofilms in 19 strains out of 24 tested strains while at 2MIC, only one strain had biofilm inhibition below 70%. The EO eliminated more than 50% of mature biofilms in 13 strains out of 24 tested. Biofilm disruption was concentration-dependent at sub-MIC and indicated good potential of C. munbyi EO as P. aeruginosa antibiofilm agent. Scanning electron microscopy (SEM) images revealed that untreated colonies had a well-developed biofilm while there was significant reduction of biofilms with distorted architecture and cell shrinkage upon treatment with EO. The C. munbyi EO at MIC and sub-MIC was able to reduce quorum-sensing (QS) virulence factors such as pyocyanin production, exopolysaccharides matrix and elastase secretion. Significant QS revealed by reduction of violacein in C. violaceum (CV12472 and CV026) was observed. Conclusion: The overall results show good anti-pseudomonal effects of C. munbyi EO which makes it a potential agent to reduce the spread of infections and resistance to antibiotics from P. aeruginosa strains.

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Disruption of Biofilm Formation and Quorum Sensing in Pathogenic Bacteria by Compounds from Zanthoxylum Gilletti (De Wild) P.G. Waterman

Cited by 11 publications

References 47 publications

Evaluation of anti-quorum sensing and antibiofilm effects of secondary metabolites from Gambeya lacourtiana (De Wild) Aubr. & Pellegr against selected pathogens

Evaluation of anti-quorum sensing and antibiofilm effects of secondary metabolites from Gambeya lacourtiana (De Wild) Aubr. & Pellegr against selected pathogens

Synthesis of a new diarylhydrazone derivative and an evaluation of its in vitro biofilm inhibition and quorum sensing disruption along with a molecular docking study

Biofilm Disruption and Virulence Attenuation Effects of Essential Oil From Endemic Algerian Cistus munbyi (Cistaceae) Against Clinical Strains of Pseudomonas aeruginosa

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