Disruption of the sheep BMPR-IB gene by CRISPR/Cas9 in in vitro -produced embryos

Zhang, Xuemei; Li, Wenrong; Wu, Yihan; Peng, Xinrong; Lou, Bian; Wang, Liqin; Liu, Mingjun

doi:10.1016/j.theriogenology.2016.10.025

Cited by 39 publications

(23 citation statements)

References 23 publications

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“…This is phenomenon is called mosaicism, as it results in mosaic individuals composed by more than one cell population. Mosaicism was initially overlooked, as it is not a common problem in the generation of murine KO models (Bermejo-Alvarez et al, 2015), but most of the publications that have performed allele screening following CRISPR direct injection in zygotes have observed mosaicism in different species such as pigs (Hai et al, 2014;Sato et al, 2015;Wang et al, 2015c;Chuang et al, 2016;Kang et al, 2016;Petersen et al, 2016;Yu et al, 2016;Zhou et al, 2016;Burkard et al, 2017;Park et al, 2017;Whitworth et al, 2017), goats ), sheep (Crispo et al, 2015Wang et al, 2016c;Vilarino et al, 2017;Zhang et al, 2017), cattle (Bevacqua et al, 2016 and rabbits (Yan et al, 2014;Honda et al, 2015;Guo et al, 2016;Lv et al, 2016;Song et al, 2016a;Song et al, 2016b;Sui et al, 2016;Yang et al, 2016;.…”

Section: Mosaicism Impairs Direct Ko Generation By Crisprmentioning

confidence: 99%

Directions and applications of CRISPR technology in livestock research

Lamas-Toranzo¹,

Ramos‐Ibeas²,

Pericuesta³

et al. 2018

Anim Reprod

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The ablation (KO) or targeted insertion (KI) of specific genes or sequences has been essential to test their roles on a particular biological process. Unfortunately, such genome modifications have been largely limited to the mouse model, as the only way to achieve targeted mutagenesis in other mammals required from somatic cell nuclear transfer, a time-and resource-consuming technique. This difficulty has left research in livestock species largely devoided of KO and targeted KI models, crucial tools to uncover the molecular roots of any physiological or pathological process. Luckily, the eruption of site-specific endonucleases, and particularly CRISPR technology, has empowered farm animal scientists to consider projects that could not develop before. In this sense, the availability of genome modification in livestock species is meant to change the way research is performed on many fields, switching from descriptive and correlational approaches to experimental research. In this review we will provide some guidance about how the genome can be edited by CRISPR and the possible strategies to achieve KO or KI, paying special attention to an initially overlooked phenomenon: mosaicism. Mosaicism is produced when the zygote´s genome edition occurs after its DNA has replicated, and is characterized by the presence of more than two alleles in the same individual, an undesirable outcome when attempting direct KO generation. Finally, the possible applications on different fields of livestock research, such as reproduction or infectious diseases are discussed. ReferencesBennett GL, Leymaster KA. 1989. Integration of ovulation rate, potential embryonic viability and uterine capacity into a model of litter size in swine. J Anim Sci, 67:1230-1241. Berg DK, van Leeuwen J, Beaumont S, Berg M, Pfeffer PL. 2010. Embryo loss in cattle between Days 7 and 16 of pregnancy. Theriogenology, 73:250-260. Berg DK, Smith CS, Pearton DJ, Wells DN, Broadhurst R, Donnison M, Pfeffer PL. 2011. Trophectoderm lineage determination in cattle. Dev Cell, 20:244-255. Bermejo-Alvarez P, Rizos D, Rath D, Lonergan P, Gutierrez-Adan A. 2010. Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts. Proc Natl Acad Sci U S A,

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Section: Mosaicism Impairs Direct Ko Generation By Crisprmentioning

confidence: 99%

Directions and applications of CRISPR technology in livestock research

Lamas-Toranzo¹,

Ramos‐Ibeas²,

Pericuesta³

et al. 2018

Anim Reprod

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“…; Park et al., ; Sato et al., ; Tao et al., ; Wang, Cao, Huang, et al. ; Wang, Du, et al., ; Wang, Zhou, et al., ; Whitworth et al., ; Yu et al., ), sheep (Crispo et al., ; Wang, Niu, et al., ; Zhang et al., ), goat (Guo et al., ; Wang, Yu, et al., ) and cattle (Bevacqua et al., ). CRISPR can be also delivered as ribonucleoprotein (Park, Powell, et al., ; Sheets et al., ), with the advantage that it acts faster than RNA delivery, as it does not require the generation of Cas9 protein by the zygote.…”

Section: State Of the Artmentioning

confidence: 99%

CRISPR is knocking on barn door

Lamas-Toranzo

Guerrero-Sánchez

Miralles-Bover

et al. 2017

Reprod Domestic Animals

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Genome modification at specific loci in livestock species was only achievable by performing homologous recombination in somatic cells followed by somatic cell nuclear transfer. The difficulty and inefficiency of this method have slowed down the multiple applications of genome modification in farm animals. The discovery of site-specific endonucleases has provided a different and more direct route for targeted mutagenesis, as these enzymes allow the ablation (KO) or insertion (KI) of specific genomic sequences on a single step, directly applied to zygotes. Clustered regularly interspaced short palindromic repeats (CRISPR), the last site-specific endonuclease to be developed, is a RNA-guided endonuclease, easy to engineer and direct to a given target site. This technology has been successfully applied to rabbits, swine, goats, sheep and cattle, situating genome editing in livestock species at an attainable distance, thereby empowering scientist to develop a myriad of applications. Genetically modified livestock animals can be used as biomodels to study human or livestock physiology and disease, as bioreactors to produce complex proteins, or as organ donors for transplantation. Specifically on livestock production, genome editing in farm animals may serve to improve productive genetic traits, to improve various animal products, to confer resistance to diseases or to minimize the environmental impact on farming. In this review, we provide an overview of the current methods for site-specific genome modification in livestock species, discuss potential and already developed applications of genome edition in farm animals and debate about the possibilities for approval of products derived from gene-edited animals for human consumption.

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“…A number of gene-edited animals have been produced through SCNT cloning technique ( Table-2 ) [ 7 , 8 , 18 , 28 , 30 - 37 ]. The first successful attempt in cattle was made by Tan et al .…”

Section: Genome Editingmentioning

confidence: 99%

“…On an average, fewer embryos are required to edit a particular gene using this approach as compared to SCNT considering the losses associated with cloning [ 26 ]. Various animal genomes have been edited through this method such as cattle, sheep, and pig ( Table-2 ) [ 7 , 8 , 18 , 28 , 30 - 37 ].. A study demonstrated that injection of TALEN mRNA into the zygote can produce MSTN gene-edited cattle and sheep [ 7 ]. The innovation is critical to the cattle industry in a sense that specific gene-edited off-springs can be produced through ovum pickup, in vitro fertilization, and zygote microinjection (OPU-IVF-ZM).…”

Section: Genome Editingmentioning

confidence: 99%

See 1 more Smart Citation

Advances in genome editing for improved animal breeding: A review

Bhat¹,

Malik²,

Ahmad³

et al. 2017

Vet World

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Since centuries, the traits for production and disease resistance are being targeted while improving the genetic merit of domestic animals, using conventional breeding programs such as inbreeding, outbreeding, or introduction of marker-assisted selection. The arrival of new scientific concepts, such as cloning and genome engineering, has added a new and promising research dimension to the existing animal breeding programs. Development of genome editing technologies such as transcription activator-like effector nuclease, zinc finger nuclease, and clustered regularly interspaced short palindromic repeats systems begun a fresh era of genome editing, through which any change in the genome, including specific DNA sequence or indels, can be made with unprecedented precision and specificity. Furthermore, it offers an opportunity of intensification in the frequency of desirable alleles in an animal population through gene-edited individuals more rapidly than conventional breeding. The specific research is evolving swiftly with a focus on improvement of economically important animal species or their traits all of which form an important subject of this review. It also discusses the hurdles to commercialization of these techniques despite several patent applications owing to the ambiguous legal status of genome-editing methods on account of their disputed classification. Nonetheless, barring ethical concerns gene-editing entailing economically important genes offers a tremendous potential for breeding animals with desirable traits.

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Disruption of the sheep BMPR-IB gene by CRISPR/Cas9 in in vitro -produced embryos

Cited by 39 publications

References 23 publications

Directions and applications of CRISPR technology in livestock research

Directions and applications of CRISPR technology in livestock research

CRISPR is knocking on barn door

Advances in genome editing for improved animal breeding: A review

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