2018
DOI: 10.1186/s13068-018-1011-5
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Disruption of zinc finger DNA binding domain in catabolite repressor Mig1 increases growth rate, hyphal branching, and cellulase expression in hypercellulolytic fungus Penicillium funiculosum NCIM1228

Abstract: BackgroundThere is an urgent requirement for second-generation bio-based industries for economical yet efficient enzymatic cocktail to convert diverse cellulosic biomass into fermentable sugars. In our previous study, secretome of Penicillium funiculosum NCIM1228 showed high commercial potential by exhibiting high biomass hydrolyzing efficiency. To develop NCIM1228 further as an industrial workhorse, one of the major genetic interventions needed is global deregulation of cellulolytic genes to achieve higher en… Show more

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Cited by 39 publications
(38 citation statements)
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“…10B to G. In accordance with the mRNA levels and earlier report (25), Pf Mig1 88 strain showed a 2-fold increase in all enzyme activities evaluated, including the total extracellular protein. It was found that Pf OAO2 and Pf OAO3 showed ∼65% increase and 27% increase in Avicelase (Fig.…”
Section: Resultssupporting
confidence: 90%
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“…10B to G. In accordance with the mRNA levels and earlier report (25), Pf Mig1 88 strain showed a 2-fold increase in all enzyme activities evaluated, including the total extracellular protein. It was found that Pf OAO2 and Pf OAO3 showed ∼65% increase and 27% increase in Avicelase (Fig.…”
Section: Resultssupporting
confidence: 90%
“…We decided to insert additional copy of PfLPMO9 gene in the background of Mig1-repressor deleted strain of P. funiculosum , i.e., Pf Mig1 88 , as it was shown to produce higher quantity of cellulolytic enzymes in our earlier study (25). To overexpress the PfLPMO9 gene in Pf Mig1 88 strain, the expression vector containing the endogenous gene along with its promoter and terminator was constructed using the backbone of pBIF binary vector.…”
Section: Resultsmentioning
confidence: 99%
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