Dissecting rapid estrogen signaling with conjugates

Shearer, Kati E.; Rickert, Emily L.; Peterson, Anton C.; Weatherman, Ross

doi:10.1016/j.steroids.2012.02.021

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…In general, three design strategies have been employed: 1) conjugation to large proteins, such as bovine serum albumin (E2-BSA); (2) attachment to oligomeric/polymeric dendrimers (E2-PAMAM) or cyclodextrins; and (3) ionic E2 derivatives (E2-NMe 3 + ). The use and cautionary experimental considerations for the use of conjugates to investigate rapid E2 signaling was recently reviewed (Shearer et al, 2012).…”

Section: Membrane-impermeable Estrogen Probesmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

2015

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Section: Membrane-impermeable Estrogen Probesmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

2015

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“…BSA-conjugated steroids have often been used in rapid signaling studies because the large structure prevents hormones from crossing the plasma membrane, thereby ensuring that the hormone exclusively binds to and activates pathways originating from membrane receptors. However, BSA itself may interfere with the biological activity of cell signaling pathways [52]. Further, the conformation of BSA may lead to interference of the lipid rafts at the point of mNR localization [53].…”

Section: Approachesmentioning

confidence: 99%

Communication between genomic and non-genomic signaling events coordinate steroid hormone actions

2018

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Steroid hormones are lipophilic molecules produced in one cell that can travel great distances within the body to elicit biological effects in another cell. In the canonical pathway, steroid hormone binding to a nuclear receptor (NR), often in the cytoplasm, causes the receptor to undergo a conformational change and translocate to the nucleus, where it interacts with specific sequences of DNA to regulate transcription. In addition to the classical genomic mechanism of action, alternate mechanisms of steroid activity have emerged that involve rapid, non-genomic signaling. The distinction between these two major mechanisms of action lies in the subcellular location of the initiating steroid hormone action. Importantly, the mechanisms of action are not exclusive, in that each can affect the activity of the other. Here, we describe the different types of genomic and non-genomic steroid hormone signaling mechanisms and how they can influence one another to ultimately regulate biology. Further, we discuss the approaches being used to study the non-genomic signaling events and address important caveats to be considered when designing new experiments. Thus, this minireview can serve as an introduction to the diverse signaling mechanisms of steroid hormones and offers initial, experimental guidance to those entering the field.

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“…Finally, other studies note that BPA exposure elicits a rapid (i.e. within 15 min of exposure) and potent estrogenic effects via nonclassical estrogen-triggered pathways (Alonso-Magdalena et al 2012, Shearer et al 2012.…”

Section: Selection Of Bpa As a Model Compoundmentioning

confidence: 99%

Do Prenatal Exposures Pose a Real Threat to Ovarian Function? BPA as a Case Study

Mathew

Mahalingaiah

2019

Reproduction

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Fetal development represents a time of potential vulnerability due to rapid cell division, organ development and limited fetal kidney/liver activity for detoxification and metabolism of exposures. Health effects of prenatal toxicant exposure have previously been described, but there is little cohesive evidence surrounding effects on ovarian function. Using bisphenol A (BPA) as a case study, we seek to examine whether a prominent prenatal environmental exposure can pose a real threat to human ovarian function. To do so, we broadly review human oogenesis and menstrual cycle biology. We then present available literature addressing prenatal bisphenol A and diverse outcomes at the level of the ovary. We highlight relevant human cohorts and mammalian models to review the existing data on prenatal exposures and ovarian disruption. Doing so suggests that while current exposures to BPA have not shown marked or consistent results, there is data sufficient to raise concerns regarding ovarian function. Challenges in the examination of this question suggest the need for additional models and pathways by which to expand these examinations in humans.

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Dissecting rapid estrogen signaling with conjugates

Cited by 7 publications

References 40 publications

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

International Union of Basic and Clinical Pharmacology. XCVII. G Protein–Coupled Estrogen Receptor and Its Pharmacologic Modulators

Communication between genomic and non-genomic signaling events coordinate steroid hormone actions

Do Prenatal Exposures Pose a Real Threat to Ovarian Function? BPA as a Case Study

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