2023
DOI: 10.1021/acssensors.3c00068
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Dissecting Sperm Mitochondrial G-Quadruplex Structures Using a Fluorescent Probe Biomarker to Monitor and Regulate Fertilization Capability

Abstract: To monitor the levels of mitochondrial DNA Gquadruplexes (mtDNA G4s) in spermatozoa and to explore the possibility using mtDNA G4s as a reliable marker in patients with multiple clinical insemination failures, a novel chemical TPE-mTO probe engineered in our previous work was used on both samples from the mice sperm and from patients with fertilization failure. Expression of valosin-containing protein and the zona-free hamster egg assay were used to evaluate mitophagy and human sperm penetration. RNA-sequencin… Show more

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Cited by 5 publications
(3 citation statements)
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“…Recently, we have discovered that increased mtG4 would also cause mitochondria damage in spermatozoa and is associated with fertilization abnormalities . Herein, PATO was applied to test mtG4 variation in spermatozoa through FCM detection (Figure A).…”
Section: Results and Discissionmentioning
confidence: 99%
See 1 more Smart Citation
“…Recently, we have discovered that increased mtG4 would also cause mitochondria damage in spermatozoa and is associated with fertilization abnormalities . Herein, PATO was applied to test mtG4 variation in spermatozoa through FCM detection (Figure A).…”
Section: Results and Discissionmentioning
confidence: 99%
“…Assisted by mtG4-specific fluorescent probes, some studies have revealed that mtDNA G4s not only affect the replication and transcription of the mitochondrial genome but also cause glycolysis enhancement . Recently, we found an increase of mtG4s in the sperms of patients with fertilization failure using our previously reported probe (TPE-mTO), and the accumulation of mtG4 in sperms will lead to mitochondrial damage. , However, the existing mtG4 probes still face some issues that restrict the exploration of mtG4-related functions, such as the binding capacity of nDNA G4s after long-term incubation (∼1 h), unsatisfactory resistance to photobleaching, the cross-talk between excitation and emission (short Stokes shift, <70 nm), and difficulty in achieving near-infrared (NIR) emission (emission wavelength exceeds 700 nm, in particular). It is therefore highly critical to develop an easily available novel core structure to obtain mtG4 targeted probes with NIR emission, prominent photostability, and large Stokes shift.…”
mentioning
confidence: 92%
“…G4 is an intriguing DNA structure that interacts with dyes . Several fluorophores preferentially bind to G4 than double-stranded DNA (dsDNA) and manifest enhanced fluorescence after binding, enabling the detection and study of G4 in various biological, chemical, and environmental contexts. The interaction between dyes and G4 structures provides insight into their structure, topology, dynamics, and microenvironment. , Thioflavin T (ThT) is extensively used for probing G4 because it remarkably enhances fluorescence in the presence of G4 . ThT belongs to the group of molecular rotors that show strong emission when ring rotation is inhibited on adopting a specific chemical configuration .…”
Section: Introductionmentioning
confidence: 99%