Dissecting the effects of antibiotics on horizontal gene transfer: Analysis suggests a critical role of selection dynamics

Lopatkin, Allison J.; Sysoeva, Tatyana A.; Li, You

doi:10.1002/bies.201600133

Cited by 58 publications

(48 citation statements)

References 132 publications

(147 reference statements)

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“…Accurate quantification of all three processes should provide a general framework to reconcile diverse outcomes and establish the role of conjugation in promoting plasmid persistence. However, confounding measurements of conjugation and growth dynamics have prevented general conclusions 34 , 35 . For instance, a high segregation error rate could be obscured by a fast conjugation efficiency 36 .…”

Section: Introductionmentioning

confidence: 99%

Persistence and reversal of plasmid-mediated antibiotic resistance

et al. 2017

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In the absence of antibiotic-mediated selection, sensitive bacteria are expected to displace their resistant counterparts if resistance genes are costly. However, many resistance genes persist for long periods in the absence of antibiotics. Horizontal gene transfer (primarily conjugation) could explain this persistence, but it has been suggested that very high conjugation rates would be required. Here, we show that common conjugal plasmids, even when costly, are indeed transferred at sufficiently high rates to be maintained in the absence of antibiotics in Escherichia coli. The notion is applicable to nine plasmids from six major incompatibility groups and mixed populations carrying multiple plasmids. These results suggest that reducing antibiotic use alone is likely insufficient for reversing resistance. Therefore, combining conjugation inhibition and promoting plasmid loss would be an effective strategy to limit conjugation-assisted persistence of antibiotic resistance.

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Section: Introductionmentioning

confidence: 99%

Persistence and reversal of plasmid-mediated antibiotic resistance

et al. 2017

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“…Comparing conjugation frequencies is a common method applied when evaluating horizontal gene transfer [40]. To calculate the CF, the amount of transconjugants is either divided by the number of recipients or donors.…”

Section: Discussionmentioning

confidence: 99%

“…Also, the impact of the environment on bacterial growth is frequently neglected. Thereby, a bias arises [40][41][42], which was circumvented in the current study by applying the stress impact factor (SIF). In the present study, the results of SIF correction were rather similar to the CF/D and CF/R values besides group LA, Formulation C and Formulation L, where differences of 0.5, 0.4, and 0.3 log cfu/mL were observed, respectively.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Direct-Fed Microbials and Phytogenic Feed Additives on Prevalence and Transfer of Extended-Spectrum Beta-Lactamase Genes in Broiler Chicken

et al. 2020

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Poultry frequently account for the highest prevalence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in livestock. To investigate the impact of direct-fed microbials (DFM) and phytobiotic feed additives on prevalence and conjugation of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, an animal trial was conducted. Lactobacillus agilis LA73 and Lactobacillus salivarius LS1 and two commercial phytogenic feed additives (consisting of carvacrol, cinnamaldehyde, and eugenol) were used as feed additives either alone or as a combination of DFM and phytogenic feed additive. An ESBL-producing E. coli donor and a potentially pathogenic Salmonella Typhimurium recipient were inoculated at 5 × 109 cells/mL in cecal contents from 2-week-old broilers. Conjugation frequencies were determined after 4 h aerobic co-incubation at 37 °C and corrected for the impact of the sample matrix on bacterial growth of donor and recipient. Surprisingly, indigenous Enterobacteriaceae acted as recipients instead of the anticipated Salmonella recipient. The observed increase in conjugation frequency was most obvious in the groups fed the combinations of DFM and phytogenic product, but merely up to 0.6 log units. Further, cecal samples were examined for ESBL-producing Enterobacteriaceae on five consecutive days in broilers aged 27–31 days. All samples derived from animals fed the experimental diet showed lower ESBL-prevalence than the control. It is concluded that Lactobacillus spp. and essential oils may help to reduce the prevalence of ESBL-harboring plasmids in broilers, while the effect on horizontal gene transfer is less obvious.

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“…the phenomenon that increasing resistance to one antibiotic leads to collateral sensitivity of another) is predictive of whether cycling two antibiotics will slow down evolution [86]. These strategies may extend beyond de novo mutation, with selection potentially governing horizontal gene transfer dynamics as well [87]. Ultimately, treatment strategy may become a balance between applying strong selective pressure (i.e.…”

Section: Treatmentsmentioning

confidence: 99%

Quantitative and synthetic biology approaches to combat bacterial pathogens

Bethke

Wang

et al. 2017

Current Opinion in Biomedical Engineering

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Antibiotic resistance is one of the biggest threats to public health. The rapid emergence of resistant bacterial pathogens endangers the efficacy of current antibiotics and has led to increasing mortality and economic burden. This crisis calls for more rapid and accurate diagnosis to detect and identify pathogens, as well as to characterize their response to antibiotics. Building on this foundation, treatment options also need to be improved to use current antibiotics more effectively and develop alternative strategies that complement the use of antibiotics. We here review recent developments in diagnosis and treatment of bacterial pathogens with a focus on quantitative biology and synthetic biology approaches.

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Dissecting the effects of antibiotics on horizontal gene transfer: Analysis suggests a critical role of selection dynamics

Cited by 58 publications

References 132 publications

Persistence and reversal of plasmid-mediated antibiotic resistance

Persistence and reversal of plasmid-mediated antibiotic resistance

The Impact of Direct-Fed Microbials and Phytogenic Feed Additives on Prevalence and Transfer of Extended-Spectrum Beta-Lactamase Genes in Broiler Chicken

Quantitative and synthetic biology approaches to combat bacterial pathogens

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