Dissecting the molecular mechanisms of producing biofuel and value-added products by cadmium tolerant microalgae as sustainable biorefinery approach

Tripathi, Shweta; Kairamkonda, Manikyaprabhu; Gupta, Payal; Poluri, Krishna Mohan

doi:10.1016/j.cej.2022.140068

Cited by 22 publications

(8 citation statements)

References 88 publications

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“…Metabolite(s) concentration data set was preprocessed using interquartile range data filtering, sample normalization using median, accompanied with square root transformation, followed by pareto scaling. − The normalized data set was analyzed using the univariate and multivariate statistical analyses in order to highlight the metabolic differences between the Rb patients and controls. Univariate analysis was performed using the Log2 fold change and t test to identify the statistically significant metabolites exhibiting the relative fold change (FC ≥ 1.4) in their concentrations among the two groups. , …”

Section: Methodsmentioning

confidence: 99%

“…Univariate analysis was performed using the Log2 fold change and t test to identify the statistically significant metabolites exhibiting the relative fold change (FC ≥ 1.4) in their concentrations among the two groups. 124,125 Multivariate statistical analysis, which includes principal component analysis (PCA), and partial least-squares discriminant analysis (PLS-DA) were conducted to identify the imperative metabolites contributing to the discrimination of the two groups. PCA is an unsupervised statistical analysis method which was used to obtain an overall grouping trend and to decode the outliers on the basis of variance across the data set.…”

Section: Nmr Data Processing and Metabolitementioning

confidence: 99%

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Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Gulati,

Manukonda,

Kairamkonda

et al. 2023

ACS Omega

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Retinoblastoma (Rb) is the most common pediatric eye cancer. To identify the biomarkers for early diagnosis and monitoring the progression of Rb in patients, mapping of the alterations in their metabolic profiles is essential. The present study aims at exploring the metabolic disparity in serum from Rb patients and controls using NMR-based metabolomics. A total of 72 metabolites, including carbohydrates, amino acids, and organic acids, were quantified in serum samples from 24 Rb patients and 26 controls. Distinct clusters of Rb patients and controls were obtained using the partial least-squares discriminant analysis (PLS-DA) model. Further, univariate and multivariate analyses of unilateral and bilateral Rb patients with respect to their age-matched controls depicted their distinct metabolic fingerprints. Metabolites including 2-phosphoglycerate, 4-aminobutyrate, proline, O -phosphocholine, O -phosphoethanolamine, and Sn-glycero-3-phosphocholine (Sn-GPC) showed significant perturbation in both unilateral and bilateral Rb patients. However, metabolic differences among the bilateral Rb cases were more pronounced than those in unilateral Rb cases with respect to controls. In addition to major discriminatory metabolites for Rb, unilateral and bilateral Rb cases showed specific metabolic changes, which might be the result of their differential genetic/somatic mutational backgrounds. This further suggests that the aberrant metabolic perturbation in bilateral patients signifies the severity of the disease in Rb patients. The present study demonstrated that identified serum metabolites have potential to serve as a noninvasive method for detection of Rb, discriminate bilateral from unilateral Rb patients, and aid in better understanding of the RB tumor biology.

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Section: Methodsmentioning

confidence: 99%

Section: Nmr Data Processing and Metabolitementioning

confidence: 99%

Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Gulati,

Manukonda,

Kairamkonda

et al. 2023

ACS Omega

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“…Using western blotting, it was found by Guo et al [22] that overweight protection was achieved by microalgae polysaccharides through increased lipolysis and decreased lipogenesis in the liver. Other investigators [23] found that the metabolic rewiring in the tricarboxylic acid (TCA) intermediates, amino acid metabolism and starch metabolism helps the cells to concentrate carbon pools for producing neutral lipids. It has also been suggested [24] that simultaneous inorganic carbon supply and pH control can be a cost-effective measure without compromising biomass and lipid productivities if the sole use of bicarbonates is applied in microalgae culture.…”

Section: Microalgae Biomassmentioning

confidence: 99%

Bioactive compounds by microalgae and potentials for the management of some human disease conditions

et al. 2023

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<abstract> <p>Microalgae biomasses are excellent sources of diverse bioactive compounds such as lipids, polysaccharides, carotenoids, vitamins, phenolics and phycobiliproteins. Large-scale production of these bioactive substances would require microalgae cultivation either in open-culture systems or closed-culture systems. Some of these bioactive compounds (such as polysaccharides, phycobiliproteins and lipids) are produced during their active growth phase. They appear to have antibacterial, antifungal, antiviral, antioxidative, anticancer, neuroprotective and chemo-preventive activities. These properties confer on microalgae the potential for use in the treatment and/or management of several neurologic and cell dysfunction-related disease conditions, including Alzheimer's disease (AD), AIDS and COVID-19, as shown in this review. Although several health benefits have been highlighted, there appears to be a consensus in the literature that the field of microalgae is still fledgling, and more research needs to be carried out to ascertain the mechanisms of action that underpin the effectiveness of microalgal compounds. In this review, two biosynthetic pathways were modeled to help elucidate the mode of action of the bioactive compounds from microalgae and their products. These are carotenoid and phycobilin proteins biosynthetic pathways. The education of the public on the importance of microalgae backed with empirical scientific evidence will go a long way to ensure that the benefits from research investigations are quickly rolled out. The potential application of these microalgae to some human disease conditions was highlighted.</p> </abstract>

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“…The development of biomarkers for various metabolic diseases has played a crucial role that predates clinical manifestations. Further, the NMR technique has also been used in algae and seaweeds recently to extract metabolite information of Cd tolerance, 17 metabolite characterization in Ulva lactuca, Gracilaria dura and Sargassum tenerrimum, 18 and signature metabolite identification of wild as well as cultured strains of G. dura. 19 The understanding of biological processes at a molecular level is pivotal in elucidating how the active conformations of molecules is achieved on differential time scales.…”

Section: Introductionmentioning

confidence: 99%

A metabolomic snapshot through NMR revealed differences in phase transition during the induction of reproduction in Ulva ohnoi (Chlorophyta)

Bodar,

Thakur,

Rajai

et al. 2024

Mol. Omics

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Dissecting the molecular mechanisms of producing biofuel and value-added products by cadmium tolerant microalgae as sustainable biorefinery approach

Cited by 22 publications

References 88 publications

Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Serum Metabolomics of Retinoblastoma: Assessing the Differential Serum Metabolic Signatures of Unilateral and Bilateral Patients

Bioactive compounds by microalgae and potentials for the management of some human disease conditions

A metabolomic snapshot through NMR revealed differences in phase transition during the induction of reproduction in Ulva ohnoi (Chlorophyta)

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