Dissecting the role of protein phosphorylation: a chemical biology toolbox

Bilbrough, Timothy S; Piemontese, Emanuele; Seitz, Oliver

doi:10.1039/d1cs00991e

Cited by 117 publications

(73 citation statements)

References 240 publications

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“…All confirmed phosphorylation residues lie in the occludin C terminal. As early as 1997, Sakakibara et al firstly observed increased phospho-serine [pSer] and phospho-threonine [pThr] occludin selectively localized to intact epithelial TJs as a detergent-insoluble form [63]. Subsequently, Kale and Elias et al confirmed occludin phosphorylation on key serine, threonine, and tyrosine residues plays a crucial role in the assembly and maintenance of TJs in Caco-2 and MDCK cells [64,65].…”

Section: Occludin Phosphorylationmentioning

confidence: 99%

The Role of Occludin in Vascular Endothelial Protection

Du¹,

Duan²,

Zhang³

2023

Endothelial Dysfunction - A Novel Paradigm

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Endothelial tight junction proteins play an important role in maintaining the integrity of vascular endothelial structure and physiological function. In recent years, studies have found that alterations in the expression, distribution, and structure of endothelial tight junction proteins may lead to many related vascular diseases and pathologies (such as diabetes, atherosclerosis, neurodegenerative diseases, and hypertension). Therefore, related strategies to prevent and/or tight junction proteins dysfunction may be an important therapeutic target. Occludin, as the most representative one among tight junction proteins, is mainly responsible for sealing intercellular junctions, maintaining cell permeability and the integrity of vascular endothelium. Here, we review the published biological information of occludin. We highlight the relationship between occludin and vascular endothelial injury-related disease. At the same time, we show our current knowledge of how vascular endothelial occludin exerts the protective effect and possible clinical applications in the future.

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Section: Occludin Phosphorylationmentioning

confidence: 99%

The Role of Occludin in Vascular Endothelial Protection

Du¹,

Duan²,

Zhang³

2023

Endothelial Dysfunction - A Novel Paradigm

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“…Phosphorylation is widespread post-translational modification of proteins and plays an important role in regulating protein and cellular functions. Although numerous phosphorylation sites have been identified, this is only the tip of the iceberg; more sites remain to be discovered [ 78 ]. Phosphorylation is most common at serine, followed by threonine and tyrosine, which occur at a frequency of approximately 11.2:2.5:1 but can also occur at other additional sites [ 79 ].…”

Section: Structure and Function Of Mitochondrial Fission Proteins Fis...mentioning

confidence: 99%

The role of Mitochondrial Fission Proteins in Mitochondrial Dynamics in Kidney Disease

Qin

Shi

2022

IJMS

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Mitochondria have many forms and can change their shape through fusion and fission of the outer and inner membranes, called “mitochondrial dynamics”. Mitochondrial outer membrane proteins, such as mitochondrial fission protein 1 (FIS1), mitochondrial fission factor (MFF), mitochondrial 98 dynamics proteins of 49 kDa (MiD49), and mitochondrial dynamics proteins of 51 kDa (MiD51), can aggregate at the outer mitochondrial membrane and thus attract Dynamin-related protein 1 (DRP1) from the cytoplasm to the outer mitochondrial membrane, where DRP1 can perform a scissor-like function to cut a complete mitochondrion into two separate mitochondria. Other organelles can promote mitochondrial fission alongside mitochondria. FIS1 plays an important role in mitochondrial–lysosomal contacts, differentiating itself from other mitochondrial-fission-associated proteins. The contact between the two can also induce asymmetric mitochondrial fission. The kidney is a mitochondria-rich organ, requiring large amounts of mitochondria to produce energy for blood circulation and waste elimination. Pathological increases in mitochondrial fission can lead to kidney damage that can be ameliorated by suppressing their excessive fission. This article reviews the current knowledge on the key role of mitochondrial-fission-associated proteins in the pathogenesis of kidney injury and the role of their various post-translational modifications in activation or degradation of fission-associated proteins and targeted drug therapy.

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“…Protein phosphorylation is an extensive strategy used for the regulation of cellular and organismal functions in eukaryotes [ 1 , 2 , 3 ]. Eukaryotes are rich in protein kinases, a class of enzymes that catalyze protein phosphorylation, and the AGC protein kinase family is one of the seven conserved protein kinase families.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the AGC Protein Kinase Gene Family Related to Photosynthesis in Rice (Oryza sativa)

Jiang

Liu

Zhou

et al. 2022

IJMS

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The cAMP-dependent protein kinase A, cGMP-dependent protein kinase G and phospholipid-dependent protein kinase C (AGC) perform various functions in plants, involving growth, immunity, apoptosis and stress response. AGC gene family is well described in Arabidopsis, however, limited information is provided about AGC genes in rice, an important cereal crop. This research studied the AGC gene family in the AA genome species: Oryza sativa ssp. japonica, Oryza sativa ssp. indica, Oryza nivara, Oryza rufipogon, Oryza glaberrima, Oryza meridionalis, Oryza barthii, Oryza glumaepatula and Oryza longistaminata were searched and classified into six subfamilies, and it was found that these species have similar numbers of members. The analysis of gene duplication and selection pressure indicated that the AGC gene family expanded mainly by segmental or whole genome duplication (WGD), with purifying selection during the long evolutionary period. RNA-seq analysis revealed that OsAGCs of subfamily V were specifically highly expressed in leaves, and the expression patterns of these genes were compared with that of photosynthesis-related genes using qRT-PCR, discovered that OsAGC9, OsAGC20, and OsAGC22 might participate in photosynthesis. These results provide an informative perspective for exploring the evolutionary of AGC gene family and its practical application in rice.

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Dissecting the role of protein phosphorylation: a chemical biology toolbox

Abstract: Phosphorylation is a key regulator of protein and cellular function. In this review, we describe tools that enable access to homogeneously phosphorylated protein and discuss examples that demonstrate how they can be applied in functional studies.

Cited by 117 publications

References 240 publications

The Role of Occludin in Vascular Endothelial Protection

The Role of Occludin in Vascular Endothelial Protection

The role of Mitochondrial Fission Proteins in Mitochondrial Dynamics in Kidney Disease

Genome-Wide Identification of the AGC Protein Kinase Gene Family Related to Photosynthesis in Rice (Oryza sativa)

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