Dissection and weighing of accessory sex glands after formalin fixation, and a 5-day assay using young mature rats are reliable and feasible in the Hershberger assay

Comparison of anti-androgenic activity of flutamide, vinclozolin, procymidone, linuron, and p, p′-DDE in rodent 10-day Hershberger assay

“…They suggested that 5 days of treatment is insufficient to detect the anti-androgenic effect of p,p -DDE. However, Yamada et al (2001) observed the anti-androgenic effect of p,p -DDE at 100 mg/kg per day after 5 days of treatment by fixation of accessory sex organs.…”

Evaluation for reliability and feasibility of the draft protocol for the enhanced rat 28-day subacute study (OECD Guideline 407) using androgen antagonist flutamide

“…We recommend dissection, trimming and weighing of organs after fixation, especially for the thyroid, pituitary gland, prostate, seminal vesicles. Formalin fixation does not interfere with interpretation of assay results (Yamada et al, 2001). Andrews et al (2001) also recommend separating the organs after fixation.…”

Screening for in vivo (anti)estrogenic and (anti)androgenic activities of Tropaeolum majus L. and its effect on uterine contractility

“…Chemical treatment was not commenced until 7 days after castration to allow for complete recovery. Seven-week-old rats (peripubertal) were chosen as this is the typical age used in several studies (Ashby and Lefevre, 2000;Yamada et al, 2001).…”