Dissemination and proliferation of neural stem cells on the spinal cord by injection into the fourth ventricle of the rat: a method for cell transplantation

Bai, Hongliang; Suzuki, Yoshihisa; Noda, Tetsuji; Wu, Sufan; Kataoka, K.; Kitada, Masaaki; Ohta, Masayoshi; Chou, Hirotomi; Idé, Chizuka

doi:10.1016/s0165-0270(03)00007-4

Cited by 51 publications

(39 citation statements)

References 15 publications

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“…Stem cells used for transplantation into the CNS include mesenchymal stem cells (hMSCs) (Satake et al, 2004), NSCs (Bai et al, 2003;Buddensiek et al, 2010) and NPCs Ohta et al, 2004, Wu et al, 2002. NSCs can be obtained from fetal brain (Gage, 2000) and from regions of the adult brain such as the hippocampal subgranular zone/ dentate gyrus and the subventricular zone (Rietze et al, 2001).…”

Section: Grafting Of Stem Cells or Neurospheres Into The Csfmentioning

confidence: 99%

“…The injected cells followed the fl ow of subarachnoidal CSF and survived as clusters on the pial surface of the spinal cord. Notably, a large number of them migrated into the lesion site, integrated into the injured spinal cord and survived within the host spinal cord for as long as 8 months without any tumorigenic changes (Wu et al, 2002(Wu et al, , 2004Bai et al, 2003). NSCs transplanted into the 4th ventricle of animals with injured dorsal roots reached the lesion root and associated with axons in the same manner as Schwann cells (Ohta et al, 2004).…”

Section: Grafting Of Stem Cells or Neurospheres Into The Csfmentioning

confidence: 99%

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A cell junction pathology of neural stem cells leads to abnormal neurogenesis and hydrocephalus

et al. 2012

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Most cells of the developing mammalian brain derive from the ventricular (VZ) and the subventricular (SVZ) zones. The VZ is formed by the multipotent radial glia/neural stem cells (NSCs) while the SVZ harbors the rapidly proliferative neural precursor cells (NPCs). Evidence from human and animal models indicates that the common history of hydrocephalus and brain maldevelopment starts early in embryonic life with disruption of the VZ and SVZ. We propose that a "cell junction pathology" involving adherent and gap junctions is a fi nal common outcome of a wide range of gene mutations resulting in proteins abnormally expressed by the VZ cells undergoing disruption. Disruption of the VZ during fetal development implies the loss of NSCs whereas VZ disruption during the perinatal period implies the loss of ependyma. The process of disruption occurs in specifi c regions of the ventricular system and at specifi c stages of brain development. This explains why only certain brain structures have an abnormal development, which in turn results in a specifi c neurological impairment of the newborn. Disruption of the VZ of the Sylvian aqueduct (SA) leads to aqueductal stenosis and hydrocephalus, while disruption of the VZ of telencephalon impairs neurogenesis. We are currently investigating whether grafting of NSCs/neurospheres from normal rats into the CSF of hydrocephalic mutants helps to diminish/repair the outcomes of VZ disruption.

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Section: Grafting Of Stem Cells or Neurospheres Into The Csfmentioning

confidence: 99%

Section: Grafting Of Stem Cells or Neurospheres Into The Csfmentioning

confidence: 99%

A cell junction pathology of neural stem cells leads to abnormal neurogenesis and hydrocephalus

et al. 2012

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“…So far, NSCs have been transplanted predominantly directly into the rodent CNS (Svendsen et al, 1997;Fricker et al, 1999;Jain et al, 2006;Lepore et al, 2006;Vazey et al, 2006;Corti et al, 2007;Darsalia et al, 2007;Kitayama et al, 2007;Lee et al, 2007;Martin and Liu, 2007;Olstorn et al, 2007;Wei et al, 2007), but, in recent reports, nontraumatic transplantation into the CNS via cerebrospinal fluid (CSF) has been demonstrated. In different studies, an intensive invasion, migration, and integration of transplanted NSCs into the damaged CNS have been detected after transplantation via CSF (Wu et al, 2002a,b;Bai et al, 2003;Ohta et al, 2004). In a very recent report, intrathecal application of neuroectodermally converted human bone marrow-derived mesodermal stromal cells (hMSC-NSCs) in the cisterna magna of an ALS mouse model has been reported (Habisch et al, 2007).…”

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confidence: 99%

Adult cerebrospinal fluid inhibits neurogenesis but facilitates gliogenesis from fetal rat neural stem cells

Buddensiek

Dressel

Kowalski

et al. 2009

J of Neuroscience Research

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Neural stem cells (NSCs) are a promising source for cell replacement therapies for neurological diseases. Administration of NSCs into the cerebrospinal fluid (CSF) offers a nontraumatic transplantation method into the brain. However, cell survival and intraparenchymal migration of the transplants are limited. Furthermore, CSF was recently reported to be an important milieu for controlling stem cell processes in the brain. We studied the effects of adult human leptomeningeal CSF on the behavior of fetal rat NSCs. CSF increased survival of NSCs compared with standard culture media during stem cell maintenance and differentiation. The presence of CSF enhanced NSC differentiation, leading to a faster loss of self-renewal capacity and faster and stronger neurite outgrowth. Some of these effects (mainly cell survival, neurite brancing) were blocked by addition of the bone morphogenic protein (BMP) inhibitor noggin. After differentiation in CSF, significantly fewer MAP2ab(+) neurons were found, but there were more GFAP(+) astroglia compared with standard media. By RT-PCR analysis, we determined a decrease of mRNA of the NSC marker gene Nestin but an increase of Gfap mRNA during differentiation up to 72 hr in CSF compared with standard media. Our data demonstrate that adult human leptomeningeal CSF enhances cell survival of fetal rat NSCs during proliferation and differentiation. Furthermore, CSF provides a stimulus for gliogenesis but inhibits neurogenesis from fetal NSCs. Our data suggest that CSF contains factors such as BMPs regulating NSC behavior, and we hypothesize that fast differentiation of NSCs in CSF leads to a rapid loss of migration capacity of intrathecally transplanted NSCs.

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“…The clinical trial of transplanting MSCs into the CSF has been used in Japanese for treatment of the subacute stage SCI, and the safety of this therapy is confirmed. Although definite improvement in the score of ASIA scale is obtained, the efficacy of this kind of cell transplantation should be evaluated by a committee with members outside this study team after a series of cases in accordance with the protocol [61,62,39] . As inflammatory process may attract targeted migration of transplanted cells into the parenchyma, and abovementioned data were come from cell transplantation during acute and subacute phase of injury, the critical issue is the time window of cell transplantation.…”

Section: Transplantation Of Cells Via Csf For Cns Regenerationmentioning

confidence: 99%

Treatment of spinal cord injury by transplantation of cells via cerebrospinal fluid

Liu

Huang

2008

Neurosci. Bull.

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It is very important to probe into the axonal regeneration and functional recovery of central nervous system (CNS) after implantation of cells into cerebrospinal fluid (CSF) for spinal cord injury (SCI). Transplantation of cells via CSF poses great potentials for SCI in clinic. Studies on administration of cells via CSF indicate that the method is safe and convenient. The method is more suitable to treating multiple lesions of the CNS since it does not produce open lesions. However, there are disputes over its promotion effects on axonal regeneration and functional recovery of spinal cord after injury; and some questions, such as the mechanisms of functional recovery of spinal cord, the proper time window of cell transplantation, and cell types of transplantation, still need to be handled. This review summarized the method of cell transplantation via CSF for treatment of SCI.

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Dissemination and proliferation of neural stem cells on the spinal cord by injection into the fourth ventricle of the rat: a method for cell transplantation

Cited by 51 publications

References 15 publications

A cell junction pathology of neural stem cells leads to abnormal neurogenesis and hydrocephalus

A cell junction pathology of neural stem cells leads to abnormal neurogenesis and hydrocephalus

Adult cerebrospinal fluid inhibits neurogenesis but facilitates gliogenesis from fetal rat neural stem cells

Treatment of spinal cord injury by transplantation of cells via cerebrospinal fluid

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