Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-Producing
 Enterobacteriaceae
 Strains Containing the
 aac(6
 ′
 )-Ib-cr
 Gene, Which Encodes an Aminoglycoside- and Fluoroquinolone-Modifying Enzyme

Machado, Elisabete; Coque, Teresa M.; Cantón, Rafael; Baquero, Fernando; Sousa, João Carlos; Peixe, Luı́sa

doi:10.1128/aac.00473-06

Cited by 96 publications

(60 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…AmpC production was confirmed by means of the antibiogram profile and by the detection of the bla CMY-2 gene through PCR. The presence of this gene enables this strain to be resistant to AMC, as AmpC-type beta-lactamase producing bacteria can inhibit the action of clavulanate (MACHADO et al, 2006;TOUATI et al, 2008;MINARINI et al, 2008;CRÉMET et al, 2011;PANIAGUA et al, 2010;HUANG et al, 2009;MAHROUKI et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

et al. 2016

View full text Add to dashboard Cite

Bacterial resistance is a primary public health concern worldwide. Within this context, pets and breeding animals act as reservoirs for multidrug-resistant bacteria (MR), such as those producing extended spectrum beta-lactamases (ESBL) and those presenting plasmid-mediated quinolone resistance (PMQR). The aim of this study was to detect the presence of ESBL and PMQR in members of the Enterobacteriaceae family, isolated from healthy sheep and dogs from non-intense farming rural properties in the Umuarama region of Paraná, Brazil. A total of 81 oral and rectal swabs from dogs and sheep from 11 small rural properties were analyzed. These swabs were inoculated in tubes containing brain heart infusion broth (BHI), and the resulting cultures were inoculated on MacConkey agar (MAC) supplemented with 10 μg/mL cefotaxime for the selection of ESBL producers. The cells were also plated on MAC supplemented with 50 μg/mL nalidixic acid for selecting quinolone-resistant enterobacteria. The bacterial isolates were subjected to biochemical identification tests, antibiograms, double-disk synergic tests, and polymerase chain reaction analysis for resistance-inducing genes (bla ESBL , qnr, and genes encoding efflux pump and acetylases). Four (5.00%) bacterial isolates (3 Escherichia coli and 1 Morganella morganii) resistant to cephalosporins and/or quinolones were identified; of these, three (75%) isolates were from sheep and one (25%) from a dog. These findings indicate the presence of MR bacteria in the normal microbiota of the animals studied. Animals colonized with such bacteria can ResumoResistência bacteriana é considerado o maior problema de saúde pública mundial da atualidade, sendo os relatos de infecções e surtos causados por bactérias multirresistentes cada vez mais frequentes na clínica veterinária e humana. Neste contexto, animais de companhia e criação podem atuar como reservatório de bactérias multirresistentes, como as produtoras de beta-lactamases de espectro estendido (ESBL) e as que apresentam resistência as quinolonas mediada por plasmídeos (PMQR). O objetivo deste trabalho foi detectar beta-lactamases de espectro estendido e resistência as quinolonas mediada por plasmídeos em membros da família Enterobacteriaceae isolados de ovinos e cães de propriedades rurais não tecnificadas da região de Umuarama, Paraná, Brasil. Foram analisados 81 swabs de cães e ovinos provenientes de 11 pequenas propriedades rurais da região de Umuarama (PR). Os swabs foram inoculados em caldo Brain Heart Infusion (BHI) e o crescimento obtivo foi em seguida semeado em placas de Petri contendo ágar MacConkey (MC) acrescido de cefotaxima 10 μg/mL para seleção de bactérias gram-negativas produtoras de ESBL; e placas contendo MC acrescido de ácido nalidíxico 50 g/mL para seleção de bactérias gram-negativas resistentes as quinolonas. Os isolados bacterianos obtidos foram submetidos a testes de antibiograma pelo método de disco-difusão em ágar, teste sinérgico do duplo-disco e reação em cadeia da polimerase (PCR) para genes que confe...

show abstract

Section: Resultsmentioning

confidence: 99%

Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

et al. 2016

View full text Add to dashboard Cite

show abstract

“…A multicountry study reported that carbapenems are the treatment of choice for infections caused by ESBL-producing K. pneumoniae (26). Coresistance to several antimicrobial agents has often been described for CTX-M-producing isolates (27,28). These arrays of resistance genes confer a multidrug resistance phenotype that can be transferred horizontally (28).…”

Section: Discussionmentioning

confidence: 99%

Strain Typing and Molecular Characterization of CTX-M-1 Group ESBL in Clinical Klebsiella pneumoniae Isolated from Children

Peerayeh

Derakhshan

Fallah

et al. 2016

Arch Pediatr Infect Dis

View full text Add to dashboard Cite

show abstract

“…Furthermore, co-expression of multiple resistance genes allows bacteria to sustain antimicrobial selection pressure; hence, clonal expansion of multidrug-resistant strains is possible under favorable conditions (14). Combating multidrug-resistant bacteria remains a tremendous challenge; therefore, periodic surveillance of emerging antimicrobial resistance and effective infection control measures are important to prevent further spread of drug-resistant organisms in hospital settings.…”

mentioning

confidence: 99%

Identification of a Novel SHV-β-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant Klebsiella pneumoniae Isolate

2013

View full text Add to dashboard Cite

Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-Producing Enterobacteriaceae Strains Containing the aac(6 ′ )-Ib-cr Gene, Which Encodes an Aminoglycoside- and Fluoroquinolone-Modifying Enzyme

Cited by 96 publications

References 16 publications

Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

Strain Typing and Molecular Characterization of CTX-M-1 Group ESBL in Clinical Klebsiella pneumoniae Isolated from Children

Identification of a Novel SHV-β-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant <i>Klebsiella pneumoniae</i> Isolate

Contact Info

Product

Resources

About

Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-Producing Enterobacteriaceae Strains Containing the aac(6 ′ )-Ib-cr Gene, Which Encodes an Aminoglycoside- and Fluoroquinolone-Modifying Enzyme

Cited by 96 publications

References 16 publications

Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

Strain Typing and Molecular Characterization of CTX-M-1 Group ESBL in Clinical Klebsiella pneumoniae Isolated from Children

Identification of a Novel SHV-&beta;-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant <i>Klebsiella pneumoniae</i> Isolate

Contact Info

Product

Resources

About

Identification of a Novel SHV-β-Lactamase Variant (SHV-144) in a Malaysian Multidrug-Resistant <i>Klebsiella pneumoniae</i> Isolate