Dissemination of a ‘rare’ extended-spectrum β-lactamase gene blaSFO-1 mediated by epidemic clones of carbapenemase-producing Enterobacter hormaechei in China

Zhou, Kai; Zhou, Yanzi; Zhang, Chuqiu; Song, Jingjie; Cao, Xiaoli; Shen, Ping; Xiao, Yonghong

doi:10.1016/j.ijantimicag.2020.106079

Cited by 15 publications

(21 citation statements)

References 23 publications

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“…Recently, reports about the coexistence of a rare ESBL gene bla SFO−1 and carbapenemase genes were increased (Zhou et al, 2020). New Delhi metallo-lactamase (NDM-1), a β-lactam hydrolase, constitutes a critical and growingly important medical issue, since its resistance trait compromises the efficacy of almost all lactams (except aztreonam), including carbapenems (Dortet et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…New Delhi metallo-lactamase (NDM-1), a β-lactam hydrolase, constitutes a critical and growingly important medical issue, since its resistance trait compromises the efficacy of almost all lactams (except aztreonam), including carbapenems (Dortet et al, 2014). Compared with other broad-spectrum β-lactamase, the bla SFO−1 gene is a low-incidence antimicrobial resistance gene and usually not subject to systematic monitoring, which puts it at risk of being missed (Zhao et al, 2015;Zhou et al, 2020). With the increase of infections caused by carbapenemaseproducing bacteria and the lack of novel antibiotics (Gurjar, 2015), polymyxins have become the last-resort therapies in the treatment of infections caused by this kind of multidrug-resistant (MDR) bacterial (Olaitan et al, 2014;Yilmaz et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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First Report of Coexistence of blaSFO–1 and blaNDM–1 β-Lactamase Genes as Well as Colistin Resistance Gene mcr-9 in a Transferrable Plasmid of a Clinical Isolate of Enterobacter hormaechei

Zhou

Wang

et al. 2021

Front. Microbiol.

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Many antimicrobial resistance genes usually located on transferable plasmids are responsible for multiple antimicrobial resistance among multidrug-resistant (MDR) Gram-negative bacteria. The aim of this study is to characterize a carbapenemase-producing Enterobacter hormaechei 1575 isolate from the blood sample in a tertiary hospital in Wuhan, Hubei Province, China. Antimicrobial susceptibility test showed that 1575 was an MDR isolate. The whole genome sequencing (WGS) and comparative genomics were used to deeply analyze the molecular information of the 1575 and to explore the location and structure of antibiotic resistance genes. The three key resistance genes (blaSFO–1, blaNDM–1, and mcr-9) were verified by PCR, and the amplicons were subsequently sequenced. Moreover, the conjugation assay was also performed to determine the transferability of those resistance genes. Plasmid files were determined by the S1 nuclease pulsed-field gel electrophoresis (S1-PFGE). WGS revealed that p1575-1 plasmid was a conjugative plasmid that possessed the rare coexistence of blaSFO–1, blaNDM–1, and mcr-9 genes and complete conjugative systems. And p1575-1 belonged to the plasmid incompatibility group IncHI2 and multilocus sequence typing ST102. Meanwhile, the pMLST type of p1575-1 was IncHI2-ST1. Conjugation assay proved that the MDR p1575-1 plasmid could be transferred to other recipients. S1-PFGE confirmed the location of plasmid with molecular weight of 342,447 bp. All these three resistant genes were flanked by various mobile elements, indicating that the blaSFO–1, blaNDM–1, and mcr-9 could be transferred not only by the p1575-1 plasmid but also by these mobile elements. Taken together, we report for the first time the coexistence of blaSFO–1, blaNDM–1, and mcr-9 on a transferable plasmid in a MDR clinical isolate E. hormaechei, which indicates the possibility of horizontal transfer of antibiotic resistance genes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First Report of Coexistence of blaSFO–1 and blaNDM–1 β-Lactamase Genes as Well as Colistin Resistance Gene mcr-9 in a Transferrable Plasmid of a Clinical Isolate of Enterobacter hormaechei

Zhou

Wang

et al. 2021

Front. Microbiol.

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“…Strains 1847 and 13755-1A, present in IncFII (pECLA) plasmid (Table 5), are associated with antibiotic resistance genes in Cronobacter, such as carbapenemase genes ∆intI1bla IMP-26 , bla CSA , bla CMA , and IS26 flanking bla SFO-1 [77,78].…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Cronobacter sakazakii Strains Isolated from Powdered Milk

Holý

Parra‐Flores

Lepuschitz

et al. 2020

Foods

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Cronobacter spp. are opportunistic pathogens of the Enterobacteriaceae family. The organism causes infections in all age groups, but the most serious cases occur in outbreaks related to neonates with meningitis and necrotizing enterocolitis. The objective was to determine the in silico and in vitro putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk (PM) in the Czech Republic. Strains were identified by MALDI-TOF MS and whole-genome sequencing (WGS). Virulence and resistance genes were detected with the Ridom SeqSphere+ software task template and the Comprehensive Antibiotic Resistance Database (CARD) platform. Adherence and invasion ability were performed using the mouse neuroblastoma (N1E-115 ATCCCRL-2263) cell line. The CRISPR-Cas system was searched with CRISPRCasFinder. Core genome MLST identified four different sequence types (ST1, ST145, ST245, and ST297) in six isolates. Strains 13755-1B and 1847 were able to adhere in 2.2 and 3.2 × 106 CFU/mL, while 0.00073% invasion frequency was detected only in strain 1847. Both strains 13755-1B and 1847 were positive for three (50.0%) and four virulence genes, respectively. The cpa gene was not detected. Twenty-eight genes were detected by WGS and grouped as flagellar or outer membrane proteins, chemotaxis, hemolysins, and invasion, plasminogen activator, colonization, transcriptional regulator, and survival in macrophages. The colistin-resistance-encoding mcr-9.1 and cephalothin-resis-encoding blaCSA genes and IncFII(pECLA) and IncFIB(pCTU3) plasmids were detected. All strains exhibited CRISPR matrices and four of them two type I-E and I-F matrices. Combined molecular methodologies improve Cronobacter spp. decision-making for health authorities to protect the population.

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“…Infections related to multidrug-resistant bacteria (MDR) have led to the use of broad-spectrum antibiotics such as third and fourth generation cephalosporins and carbapenems, causing an increase on selective pressure that has favored the proliferation and dissemination of resistant strains [4,5]. Extended-spectrum beta-lactamases (ESBL) producing Enterobacteriaceae are considered by the World Health Organization (WHO) as priority microorganisms for the development of new antibiotics, as well as for microbial resistance monitoring [6]. ESBL production confers resistance to most penicillins, to rst, second, and third generation cephalosporins and to monobactams [7,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…ESBLs groups includes nine structural families: TEM, SHV, CTX-M, PER, VEB, GES, TLA, BES and OXA [11]. The TEM, SHV and CTX-M families are the most prevalent worldwide reported in hospital and community infections [6,12,13]. ESBLs contained in conjugative plasmids facilitate their mobility through horizontal gene transfer [11,14,15] and in turn, these plasmids may contain resistance related genes to other antibiotics, such as quinolones, aminoglycosides, tetracyclines and even colistin, which increases the chances of failure in treatment schemes [16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Detection of Multidrug-resistant Extended-spectrum Beta-lactamase-producing Enterobacteria from Community Infections in the city of Reynosa, Tamaulipas Mexico

Cruz-González¹,

Ramírez‐Moreno²,

Ortega-Balleza³

et al. 2020

Preprint

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Production of extended spectrum beta-lactamases (ESBL) is one of the main problems related to antimicrobial resistance worldwide, with the CTX-M, TEM and SHV types standing out as the most prevalent. These enzymes are usually related to plasmids which facilitates their horizontal genetic transmission. In the northeast region of Tamaulipas their clinical prevalence is unknown. Therefore, the aim of this work was to define the molecular epidemiology of ESBL-producing Enterobacteriaceae in clinical strains collected in Reynosa Tamaulipas, Mexico. A selection of 123 Enterobacteriaceae strains from different clinical patients were collected from August 2018 to December 2019. These strains were phenotypically identified by double disk synergy tests (DDST) and subsequently subjected to polymerase chain reaction for the detection and amplification of the blaTEM, blaSHV, blaCTX−M−1, blaCTX−M−2, blaCTX−M−9 and blaCTX−M−8/25 genes. Lastly, antimicrobial resistance profiles were determined by plate diffusion method and their capacity to transfer this sort of resistance by conjugation was assessed. Our results showed a prevalence of 48.78% (60/123) of ESBL-producing enterobacteria, with the blaTEM and blaCTX−M−1 genes most commonly detected in 76.67% (46/60) and 58.33% (35/60), respectively. Additionally, a 68.33% (41/60) of these ESBL-producing Enterobacteriaceae were multidrug-resistant, while 51.67% (31/60) were able to transfer some genes related to ESBL production, being blaCTX−M−1 the most common. This is the first study in the region that evaluates ESBL production in clinical Enterobacteriaceae strains, as well as the content of genes related to this phenotype and the ability to transfer this type of antimicrobial resistance.

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Dissemination of a ‘rare’ extended-spectrum β-lactamase gene blaSFO-1 mediated by epidemic clones of carbapenemase-producing Enterobacter hormaechei in China

Cited by 15 publications

References 23 publications

First Report of Coexistence of blaSFO–1 and blaNDM–1 β-Lactamase Genes as Well as Colistin Resistance Gene mcr-9 in a Transferrable Plasmid of a Clinical Isolate of Enterobacter hormaechei

First Report of Coexistence of blaSFO–1 and blaNDM–1 β-Lactamase Genes as Well as Colistin Resistance Gene mcr-9 in a Transferrable Plasmid of a Clinical Isolate of Enterobacter hormaechei

Molecular Characterization of Cronobacter sakazakii Strains Isolated from Powdered Milk

Detection of Multidrug-resistant Extended-spectrum Beta-lactamase-producing Enterobacteria from Community Infections in the city of Reynosa, Tamaulipas Mexico

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