Dissemination of a single Vibrio cholerae clone in cholera outbreaks during 2005 in Iran

Pourshafie, Mohammad Reza; Bakhshi, Bita; Ranjbar, Reza; Sedaghat, Manijeh; Sadeghifard, Nourkhoda; Yazdi, J. Zaemi; Parzadeh, Masoumeh; Raesi, J.

doi:10.1099/jmm.0.47218-0

Cited by 44 publications

(37 citation statements)

References 18 publications

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“…Our previous studies in Iran had shown a limited number of V. cholerae clones during 2002 and 2005 [1,7]. The aim of this study was to determine the genetic and phenotypic interrelationships of the V. cholerae isolates referred to our laboratory from different provinces in Iran during the cholera outbreaks (2004Á2007).…”

Section: Introductionmentioning

confidence: 98%

Assessing clonality of Vibrio cholerae strains isolated during four consecutive years (2004 – 2007) in Iran

Bakhshi

Pourshafie

2009

Scandinavian Journal of Infectious Diseases

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Genotypic and phenotypic characterizations of 50 clinical Vibrio cholerae isolates obtained during 4 consecutive y from 2004 to 2007 in Iran were studied. Antimicrobial susceptibility test, biochemical fingerprinting with Phene Plate system (PhP-RV) and pulsed-field gel electrophoresis (PFGE) were performed. Antibiotic susceptibility test of all isolates showed 12 different profiles. The predominant antimicrobial resistance profile (62%) was simultaneous resistance to SXT, streptomycin, chloramphenicol and erythromycin. PFGE revealed a total of 9 pulsotypes with a single dominant type in each y under study. PhP-RV revealed 6 common and 10 single types constituting 80% and 20% of the isolates, respectively. Diversity index of PhP-RV was 0.853, indicative of homogeneity among the isolates. Data obtained from PhP-RV was in close agreement with the results of PFGE genotyping. A comparison of the published PFGE patterns performed using the PulseNet protocol revealed the presence of similar patterns between some of our isolates and the isolates from Pakistan, Nepal and India, suggestive of dissemination of common V. cholerae clones in this region of the world. This could, in part, be due to human travel or occurrence of analogous DNA rearrangements, resulting in the emergence of similar V. cholerae genotypes in regional countries.

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Section: Introductionmentioning

confidence: 98%

Assessing clonality of Vibrio cholerae strains isolated during four consecutive years (2004 – 2007) in Iran

Bakhshi

Pourshafie

2009

Scandinavian Journal of Infectious Diseases

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“…V. cholerae strains were isolated from randomly selected cholera patients. Samples were processed as described previously (Pourshafie et al, 2007). Other bacterial strains used in the study were V. cholerae O1 (ATCC 14033), V. cholerae O139 (NICED), Bacillus cereus (ATCC 10876), Escherichia coli (ATCC 11775), Salmonella paratyphi A (MTCC 735), Salmonella typhimurium (MTCC 98), Shigella dysenteriae (NICED), Shigella flexneri (MTCC 1457), Staphylococcus aureus (ATCC 12600), Vibrio fischeri (MTCC 1738) and Vibrio parahaemolyticus (MTCC 451).…”

Section: Methodsmentioning

confidence: 99%

A large cholera outbreak due to a new cholera toxin variant of the Vibrio cholerae O1 El Tor biotype in Orissa, Eastern India

Kumar

Jain

Goel

et al. 2009

Journal of Medical Microbiology

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A total of 32 Vibrio cholerae isolates were collected during a recent large cholera outbreak in Eastern India. Biochemical and serological studies revealed that all of the isolates belonged to serogroup O1, biotype El Tor, serotype Ogawa. Two multiplex PCR assays confirmed the presence of various toxigenic and pathogenic genes -ace, ctxAB, hlyA, ompU, ompW, rfbO1, rtx, tcp, toxR and zot -in all of the isolates. Sequencing of the ctxB gene from the isolates revealed a novel mutation in the gene. Sequencing also confirmed the presence of altered cholera toxin B of the classical biotype in all of the El Tor isolates, suggesting infection of isolates by classical CTXW. The molecular diversity of V. cholerae isolates studied by enterobacterial repetitive intergenic consensus sequence PCR, BOX-PCR and randomly amplified polymorphic DNA analysis uniformly showed the clonal relationship among the outbreak V. cholerae O1 isolates. The results of this study suggest that cholera-causing V. cholerae strains are constantly evolving in epidemic areas, highlighting the potential of the emergence of more virulent strains.

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“…Clinical samples were processed for isolation of V. cholerae as described previously (Pourshafie et al, 2007). V. cholerae isolates from different outbreaks were grown on BHI agar plate for 16-18 h at 37°C and a loopful of bacterial culture was suspended in 500 µℓ de-ionised water.…”

Section: Processing Of Clinical Samplesmentioning

confidence: 99%

Detection and confirmation of toxigenic <i>Vibrio cholerae</i> O1 in environmental and clinical samples by a direct cell multiplex PCR

Yadava¹,

Jain²,

Ak³

2013

WSA

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Epidemic cholera caused by toxigenic Vibrio cholerae O1 is a major health problem in several developing countries. Traditional methods for identifying V. cholerae involve cultural, biochemical and immunological assays which are cumbersome and often take several days to complete. In the present study, a direct cell multiplex PCR was developed targeting the ompW, ctxB and rfbO1 genes for confirmation of V. cholerae, its toxigenicity and serogroup O1, respectively from clinical and environmental samples. The detection sensitivity of the multiplex PCR was 1.9 x 10 3 V. cholerae per PCR reaction. A total of 31 environmental samples and 45 clinical V. cholerae isolates from different outbreaks were examined by the PCR. The assay was simple and specific, as there was no requirement for DNA extraction and no amplification was observed with other homologous bacteria used. The assay can be very useful for rapid surveillance of toxigenic V. cholerae O1 in environmental water samples, as well as for confirmation of clinical isolates.

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Dissemination of a single Vibrio cholerae clone in cholera outbreaks during 2005 in Iran

Cited by 44 publications

References 18 publications

Assessing clonality of Vibrio cholerae strains isolated during four consecutive years (2004 – 2007) in Iran

Assessing clonality of Vibrio cholerae strains isolated during four consecutive years (2004 – 2007) in Iran

A large cholera outbreak due to a new cholera toxin variant of the Vibrio cholerae O1 El Tor biotype in Orissa, Eastern India

Detection and confirmation of toxigenic <i>Vibrio cholerae</i> O1 in environmental and clinical samples by a direct cell multiplex PCR

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