Dissemination of Cefotaxime-M-Producing <i>Escherichia coli</i> Isolates in Poultry Farms, But Not Swine Farms, in China

Li, Jingyun; Ma, Yue; Hu, Changqin; Shao-hong, Jin; Zhang, Qingsheng; Ding, Hong; Lu, Ran; Cui, Shenghui

doi:10.1089/fpd.2010.0581

Cited by 41 publications

(26 citation statements)

References 22 publications

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“…The diverse CTX-M enzymes detected in our study confirmed that the globally prevalent CTX-M-14 (22,42) and CTX-M-15 (32,37) enzymes are quite common in China, as reported previously (46). CTX-M-22, CTX-M-27, and CTX-M-65 have been reported on relatively few occasions (46), but mainly from China, and all have previously been observed among food animals in China (23,25,39). Thus, the detection of these enzymes in our isolates may indicate that food animals are an important reservoir for cephalosporin-resistant E. coli in China.…”

Section: Discussionsupporting

confidence: 90%

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Cao

Cavaco

et al. 2011

J Clin Microbiol

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A total of 222 urinary Escherichia coli isolates from 20 tertiary hospitals in 15 different provinces and 4 municipalities in mainland China were characterized by antimicrobial susceptibility, phylogrouping, and the presence of plasmid-mediated quinolone resistance genes. A subset of 138 suspected extended-spectrum cephalosporinase (ESC) producers were examined for genes encoding cephalosporin resistance. Forty-three isolates harboring bla CTX-M-14 or bla CTX-M-15 were analyzed by pulsed-field gel electrophoresis (PFGE), and plasmids containing these genes were typed using PCR-based replicon typing (PBRT). Thirteen phylogroup B2 bla CTX-M-14 -and bla CTX-M-15 -positive isolates were analyzed by multilocus sequence typing (MLST). A frequent occurrence of resistance (>46%) was observed toward cephalosporins, gentamicin, and fluoroquinolones. Among the 222 isolates, 4 qnrS1, 4 qepA, and 16 aac(6)-Ib-cr genes were confirmed. Four major phylogroups (A, B1, B2, and D) and nontypeable isolates (NTs) were found among the isolates, with phylogroup D (54%) being the most common phylogroup. A total of 110 (80%) of the 138 screened isolates harbored bla CTX-M genes, with bla CTX-M-14 (71%) and bla CTX-M-15 (24%) being the most prevalent of these genes. Nine of the 13 CTX-M-15-or CTX-M-14-containing B2 isolates belonged to ST131. PFGE typing showed a high level of diversity, and plasmid analysis indicated a very large pool of different resistance plasmids mediating the spread of bla CTX-M genes in mainland China. An equally very high frequency of resistance and equally high levels of diversity in phylogroups, PFGE types, and plasmids were observed among community-and hospitalacquired E. coli isolates, indicating the presence of a large reservoir in the community and a long-term spread of cephalosporin resistance in China.

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Section: Discussionsupporting

confidence: 90%

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Cao

Cavaco

et al. 2011

J Clin Microbiol

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“…The ESBLs detected in food-producing animals or food in other non-European countries were: China (CTX-M-3, -13, -14, -15, -22, -24, -55, -64, -65; SHV-2, -12; TEM-52), Japan (CTX-M-2 and -14), and Senegal (SHV-12) (Ben Slama et al, 2010;Cardinale et al, 2001;Doi et al, 2009;Duan et al, 2006;Jouini et al, 2007;Kojima et al, 2005;Li et al, 2010a;Li et al, 2010b;Lim et al, 2009;Matsumoto et al, 2007;Rayamajhi et al, 2008;Shiraki et al, 2004;Tian et al, 2008;Wittum et al, 2010). These data show that mainly in Asian countries a higher diversity of CTX-Mvariants was observed.…”

Section: Esbl-subtypes Detected In E Coli and Salmonella From Food-pmentioning

confidence: 99%

“…These data show that mainly in Asian countries a higher diversity of CTX-Mvariants was observed. The presence of CTX-M-15 in E. coli of healthy and sick poultry and pigs in China and Korea may reflect a different epidemiology of ESBLs in these countries (Li et al, 2010a;Li et al, 2010b;Lim et al, 2009;Tian et al, 2008).…”

Section: Esbl-subtypes Detected In E Coli and Salmonella From Food-pmentioning

confidence: 99%

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

2011

EFSA Journal

246

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The potential contribution of food-producing animals or foods to public health risks by ESBL and/or AmpCproducing bacteria is related to specific plasmid-mediated ESBL and/or AmpC genes encoded by a number of organisms. The predominant ESBL families encountered are CTX-M, TEM, and SHV; the predominant AmpCfamily is CMY. The most common genes associated with this resistance in animals are bla CTX-M-1 (the most commonly identified ESBL), and bla CTX-M-14 , followed by bla and bla Among the genes encoding AmpC-type β-lactamases, bla CMY-2 is the most common.The bacterial species most commonly identified with these genes are Escherichia coli and non-typhoidal Salmonella. ESBL/AmpC transmission is mainly driven by integrons, insertion sequences, transposons and plasmids, some of which are homologous in isolates from both food-production animals and humans. Cefotaxime is used as the drug of choice for optimum detection of bla ESBL and/or bla AmpC genes. The preferred method for isolation of ESBL-and/or AmpC-producers is screening on selective agar preceded by selective enrichment in a broth.The establishment of risk factors for occurrence of ESBL/AmpC-producing bacteria is particularly complicated by the data unavailability or lack of its accuracy. The use of antimicrobials is a risk factor for the selection and spread of resistant clones, resistance genes and plasmids. Since most ESBL-and AmpC-producing strains carry additional resistances to other commonly-used veterinary drugs, generic antimicrobial use is a risk factor for ESBL/AmpC and it is not restricted specifically to the use of cephalosporins. An additional risk factor is extensive trade of animals in EU MS. There are no data on the comparative efficiency of individual control options in reducing public health risks caused by ESBL and/or AmpC-producing bacteria related to food-producing animals. Prioritisation is complex, but it is considered that a highly effective control option would be to stop all uses of cephalosporins/systemically active 3 rd /4 th generation cephalosporins, or to restrict their use (use only allowed under specific circumstances). As co-resistance is an important issue, it is also of high priority to decrease the total antimicrobial use in animal production in the EU. KEY WORDSResistance, ESBLs, AmpC, occurrence, transmission, control options, public health microbiology. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum beta (β)-lactamases (ESBL) and/or AmpC β-lactamases (AmpC) in food and foodproducing animals. In particular, the Panel was asked: (i) to propose a definition of the ESBL-and/or AmpC-producing bacterial strains and genes relevant for public health and linked to food-producing animals or food borne transmission; (ii) to review the information on the epidemiology of acquired resistance to broad spectrum cephalosporins including the genes coding for ...

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“…CTX-M-55 first isolated from E. coli in Thailand [33] developed from one point mutation of CTX-M-15, leading to replacement of amino acid 80.In recent years, CTX-M-55 in E. coli was separated from food animals and pets in China [33][34][35][36][37], but was very rare in clinical isolates. Yu et al [10] first detected it in S. Typhimurium from the stool of a child in 2011.…”

Section: Discussionmentioning

confidence: 99%

“…Gallati et al [40] [42], Guangzhou [43], and Beijing [35] in China. The blaCTX-M-65 gene detected in our investigation was the first time this gene was isolated in Salmonella from infected humans.…”

Section: Discussionmentioning

confidence: 99%

Prevalence, resistance, and extended-spectrum beta-lactamases among Salmonella from patients in northeast China

Zhang¹,

Wang²,

Li³

et al. 2017

J Infect Dev Ctries

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Introduction: Salmonella is an important infection in Liaoning Province. Serotype and resistance levels are poorly studied. Knowledge of cephalosporin-resistant genes is needed to provide information to support the clinical diagnosis and to guide therapy. Methodology: A total of 172 isolates were collected from January 2009 to March 2014. Resistance to cephalosporin, fluoroquinolones, and other conventional first-line antibiotics was detected using the disk diffusion and agar dilution methods. The relatedness of isolates with the same serotype was analyzed by pulsed-field gel electrophoresis (PFGE). Genes encoding extended-spectrum β-lactamase enzymes (blaCTX-M, blaTEM, blaSHV, and blaOXA) were identified by polymerase chain reaction (PCR) and sequencing in cephalosporin-resistant strains. Results: S. Enteritidis, 1,4,[5],12:i:-, and S .Typhimurium were the most common serotypes. The resistance rate of Salmonella to conventional first-line antibiotics sulfamethoxazole-trimethoprim, tetracycline, chloramphenicol, and ampicillin was 21%,33%,37%, and 55%,respectively. To fluoroquinolones (gatixacin, levofloxacin, and ciprofloxacin), the resistance rates were 7%,18%, and 23%, respectively, whereas for extended-spectrum cephalosporins(ceftazidime, cefepime, and ceftriaxone), there sistance rates were 4%,8%, and 9%. In total, 14blaCTX-M, 14 blaTEM-1, and 1blaOXA-1 were found in 15 Salmonella isolates resistant to cephalosporin. No blaSHVwas detected. The blaCTX-M-55 gene was the most frequently encountered gene. The blaCTX-M-65 gene from S. Enteritidis is described for first time in Salmonella from humans. Results of PFGE indicated strong similarity among Salmonella isolates in Liaoning Province. Conclusions: Extended-spectrum cephalosporins are the first choice for the treatment of infection with Salmonella, but blaCTX-M-encoded resistance, including blaCTX-M-65, is present.

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Dissemination of Cefotaxime-M-Producing Escherichia coli Isolates in Poultry Farms, But Not Swine Farms, in China

Cited by 41 publications

References 22 publications

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

Prevalence, resistance, and extended-spectrum beta-lactamases among Salmonella from patients in northeast China

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