Dissemination of macrolides, fusidic acid and mupirocin resistance among <i>Staphylococcus aureus</i> clinical isolates

Liu, Xingmei; Deng, Shanshan; Huang, Jinmei; Huang, Yaling; Zhang, Yu; Yan, Qin; Wang, Yanhong; Li, Yanyue; Sun, Chengfu; Xu, Jin

doi:10.18632/oncotarget.19491

Cited by 21 publications

(14 citation statements)

References 74 publications

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“…Interestingly, we also determined that the majority of cfr -positive MRSA isolates harbored optrA , which is in agreement with previous reports suggesting that optrA and cfr coexist ( Li et al, 2016 ; Fan et al, 2017 ). In addition to optrA , our study cfr -positive MRSA isolates also co-carried fexA and ermC , which is also consistent with previous studies ( Liu .X et al, 2017 ). This linked the cotransmission of fexA and ermA-C with cfr gene in diverse plasmids from coagulase-negative Staphylococci as well as Enterobacteriaceae of different origins ( Wang et al, 2012a , 2013 ; Ye et al, 2015 ).…”

Section: Discussionsupporting

confidence: 93%

Characterization of the Multi-Drug Resistance Gene cfr in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated From Animals and Humans in China

Zhou

et al. 2018

Front. Microbiol.

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We investigated cfr-positive and -negative MRSA strains isolated from animals and humans in different geographical areas of China, from 2011 to 2016. Twenty cfr-positive strains (15.6%) were identified from 128 MRSA strains including 17 from food animals and three from humans. The resistance rates and prevalence of the tested antibiotic resistance genes (ARGs) in the cfr-positive MRSA isolates were higher than that in the cfr-negative MRSA isolates. All cfr-positive MRSA isolates were co-carrying fexA and ermC, and had significantly higher optrA incidence rate vs. the cfr-negative isolates (P < 0.05). In addition, multilocus sequence typing (MLST) assays showed that ST9 and spa-type t899 were the most prevalent ST and spa types in the study strains. However, all of the 20 cfr-positive and 10 randomly selected cfr-negative MRSA isolates were clonally unrelated as determined by pulsed-field gel electrophoresis (PFGE) analyses. Importantly, the cfr gene was successfully transferred to a recipient Staphylococcus aureus strain RN4220 from 13 of the 20 cfr-positive MRSA isolates by electroporation. Among these 13 cfr-positive MRSA isolates, two different genetic contexts surrounding cfr were determined and each was associated with one type of cfr-carrying plasmids. Of note, the predominant genetic context of cfr was found to be a Tn558 variant and locate on large plasmids (∼50 kb) co-harboring fexA in 11 of the 13 MRSA isolates. Furthermore, the cfr gene was also identified on small plasmids (∼ 7.1 kb) that co-carried ermC in two of the 13 MRSA isolates. Our results demonstrated a high occurrence of multi-drug resistance in cfr-positive MRSA isolates, and the spread of cfr might be attributed to horizontal dissemination of similar cfr-carrying transposons and plasmids.

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Section: Discussionsupporting

confidence: 93%

Characterization of the Multi-Drug Resistance Gene cfr in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated From Animals and Humans in China

Zhou

et al. 2018

Front. Microbiol.

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“…Liu [39]. Similar results were exhibited by Liu X et al [40], they found that the resistance rate of fusidic acid (2.9%) was much lower than mupirocin (17.6%). According to our study, most of the patients were topically administered with mupirocin rather than fusidic acid, frequent application of mupirocin may contribute to the induction of the drug resistance in our district.…”

Section: Discussionsupporting

confidence: 80%

Antibiotic-resistant profile and the factors affecting the intravenous antibiotic treatment course of generalized Staphylococcal Scalded Skin Syndrome: A retrospective study

Yang

Wang

Cao

et al. 2021

Preprint

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Background: Staphylococcal Scalded Skin Syndrome (SSSS) is caused by a special type of Staphylococcus aureus (S.aureus) which can produce exfoliative toxins. The generalized SSSS is recommended to be admitted and treated with intravenous antibiotics. However, there were limited reports on whether personal and clinical factors can have impacts on the duration of intravenous antibiotic application for pediatric patients with generalized SSSS.We performed a study to assess the factors affecting intravenous antibiotic treatment course of SSSS patients. Additionally, the positive culture rates of S.aureus in different samples and the antibiotic-resistant profile were investigated. Methods: 219 patients with generalized SSSS were included. Gender, age, area, season, maximum axillary temperature, white blood cell(WBC) count, C-reactive protein (CRP) level, types of intravenous antibiotics, and types of external antibiotics were recorded as the baseline. Simple linear regression was applied in the univariate analysis to determine the variables with statistical significance and then these variables were further examined in multivariate linear regression model. The positive culture rates of S.aureus in different samples and the drug sensitivity results were statistically compared by Fisher’s exact test and pairwise Chi square test respectively. Results: According to the multiple linear regression, older ages (β=-0.01, p <0.05) and external application of fusidic acid (β=-1.57, p <0.05) were associated with shorter treatment course, elevated leukocytes (β=0.11, p <0.001) and CRP level (β=1.64, p <0.01) were associated with longer treatment course. The positive culture rates of periorificial (54.55%) and throat swabs (30.77%) were higher than the ones of blood samples (5.97%) (p <0.01). The resistant rates of levofloxacin (8.33%), gentamycin (8.33%), tetracycline (25%), oxacillin (8.33%), vancomycin (0%) were significantly lower than the ones of erythromycin (100%), trimethoprim-sulfamethoxazole (TMP/SMX) (83.33%), clindamycin (91.67%), penicillin G(100%) (p <0.001).Conclusion: Elevated leukocytes and CRP level indicated prolonged intravenous antibiotic treatment course. Older ages and external application of fusidic acid helped to reduce the treatment course. Periorificial and throat swabs were useful to identify causative S.aureus. Oxacillin and vancomycin resistance was rare and clindamycin resistance was common. Clindamycin monotherapy for SSSS should be avoided.

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“…The ST395 lineage expressing GroP-α-GalNAc WTA was unable to undergo HGT with typical S. aureus but may have an increased capacity for acquiring mobile genetic elements, including SCCmec and some virulence genes, from CoNS. ST630 clone has been reported in many previous studies (Yu et al, 2015;Liu et al, 2017), but none of them have described their special WTA phenotypes. We hypothesize that the special tarP-/tarS-/tarM+/tagN+ ST630 clone might serve as a hub for the continuous HGT of multiple MGEs such as antimicrobial resistance and pathogenicity islands (SaPIs) between both S. aureus and CoNS.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characteristics, Virulence Gene and Wall Teichoic Acid Glycosyltransferase Profiles of Staphylococcus aureus: A Multicenter Study in China

et al. 2020

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Staphylococcus aureus (S. aureus) constantly evolves under host and environment pressures. The monitoring network is essential in assessing the epidemiology of S. aureus infections. A total of 555 S. aureus isolates were collected from five hospitals in three different geographical regions of China for the investigation of molecular characteristics, antibiotic resistance, virulence gene, and wall teichoic acid (WTA) glycosyltransferase gene profiles. 233 (42.0%) isolates were identified as MRSA, and 323 (58.2%) were defined as multidrug-resistant (MDR) isolates. MRSA prevalence showed no significant difference among the three regions. In contrast, the MDR prevalence was significantly higher in central China than that in northern China (63.5% vs. 50.8%, P < 0.05). Thirty-eight sequence types (STs) belonging to 17 clone complexes (CCs) and 126 distinct spa-types were identified. The most prevalent clone was ST59-t437 (9.7%, 54/555), followed by ST22-t309 (7.6%, 42/555) and ST5-t2460 (7.2%, 40/555). Most ST59-t437 and ST5-t2460 were MRSA isolates, whereas most ST22-t309 was MSSA isolates. The predominant clones varied in different geographical areas. The distribution of the pvl, etb, tsst, clfb, sdrC, sdrD, hlg, fnbA, and hla genes showed significant differences among different regions. We found five WTA glycosyltransferase gene profiles, with tarP-/tarS+/tarM-/tagN-being the most common combination. Remarkably, the tarP gene was identified in more CCs than just CC5 and CC398. All of 16 tarP-positive isolates also contained the tarS. Moreover, tarS was present in almost all S. aureus isolates except 10 ST630 isolates. The tagN gene was only detected in 10 of 12 ST630 S. aureus isolates without tarS. The tarM gene was absent in CC5 and CC398. In brief, there were regional differences among

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Dissemination of macrolides, fusidic acid and mupirocin resistance among Staphylococcus aureus clinical isolates

Cited by 21 publications

References 74 publications

Characterization of the Multi-Drug Resistance Gene cfr in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated From Animals and Humans in China

Characterization of the Multi-Drug Resistance Gene cfr in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated From Animals and Humans in China

Antibiotic-resistant profile and the factors affecting the intravenous antibiotic treatment course of generalized Staphylococcal Scalded Skin Syndrome: A retrospective study

Molecular Characteristics, Virulence Gene and Wall Teichoic Acid Glycosyltransferase Profiles of Staphylococcus aureus: A Multicenter Study in China

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