Dissemination of New Delhi metallo-β-lactamase-1-producing Acinetobacter baumannii in Europe

Abstract

Multidrug-resistant and New Delhi metallo-β-lactamase 1 (NDM-1) -producing Acinetobacter baumannii are increasingly reported. A collection of five NDM-1-positive A. baumannii isolates recovered in four European countries were analysed. Genotyping was performed by pulsed-field gel electrophoresis, multiplex PCR sequence typing, Diversilab and multilocus sequence typing. Three distinct sequence types were identified. All isolates harboured a chromosomally located bla(NDM-1) gene within a Tn125-like transposon. One isolate co-expressed another unrelated carbapenemase OXA-23. This report constitutes the first epidemiological study of NDM-1-producing A. baumannii from four countries.

Outbreak of NDM-1-producing Acinetobacter baumannii in France, January to May 2013

“…Genotypic comparison by pulsed-field gel electrophoresis using restriction enzyme SmaI revealed an indistinguishable profile (data not shown). Diversilab (bioMérieux, France) analysis and multilocus sequence typing (MLST) typing confirmed that these isolates were clonally related and belonged to the same sequence type, ST85 (Figure 2) [8]. The genetic environment of blaNDM-1 was investigated as previously described [8] and showed that it was located in the composite transposon Tn125 made of two copies of insertion sequence (IS) ISAba125.…”

“…Since 2010, NDM-producing MDR-AB has been identified in various parts of the world, in particular in North Africa and the Middle East [8,[13][14][15][16][17]. A series of imported cases have been identified recently in Europe, such as in the Czech Republic, Germany, Slovenia, Switzerland and Belgium [8,13,14,17].…”

“…A series of imported cases have been identified recently in Europe, such as in the Czech Republic, Germany, Slovenia, Switzerland and Belgium [8,13,14,17]. In France, the emergence of an NDM-1-producing MDR-AB strain originating from North Africa was recently highlighted [15,17].…”

“…The isolates from the hospitalised patients were compared with a collection of characterised strains [8,17]. A similarity line (89.4%) shows the cut-off to separate different clones.…”

CHROMagar Acinetobacter medium for detection of carbapenemase-producing Acinetobacter spp. strains from spiked stools

“…Carbapenemases in Acinetobacter spp. belong to Ambler class A (KPC and some GES variants) (Bonnin et al, 2013b;Robledo et al, 2010), class B (VIM, IMP, SIM, NDM) (Bonnin et al, 2012;Poirel and Nordmann, 2006), and mostly class D (oxacillinases) (Bonnin et al, 2013a). Five main groups of oxacillinases with carbapenemase activity have been identified in A. baumannii, i.e., the intrinsic chromosomal OXA-51-like and the acquired chromosomally and plasmid-encoded OXA-23-like, OXA-40-like, OXA-58-like, and OXA-143-like enzymes (Bonnin et al, 2013a;Higgins et al, 2009;Poirel and Nordmann, 2006).…”