Dissemination of Quinupristin-Dalfopristin and Linezolid resistance genes among hospital environmental and healthy volunteer fecal isolates of Enterococcus faecalis and Enterococcus faecium

Malidareh, Elham Boodaghi; Ahanjan, Mohammad; Marghmalek, Saba Asgharzadeh; Goli, Hamid Reza

doi:10.1007/s11033-022-07627-3

Cited by 4 publications

(4 citation statements)

References 46 publications

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“…To the best of our knowledge, this is the first study performing Boodaghi Malidareh et al, 2022;Miller et al, 2014). Almost all sequenced E. faecium strains in this study, except strain E5-100, carried the msr(C) gene.…”

Section: Discussionmentioning

confidence: 89%

“…Our results are also in accordance with the high resistance prevalence against quinupristin/dalfopristin in E. faecium from healthy food‐producing animals in Europe (de Jong et al, 2019 ). Quinupristin/dalfopristin resistance in E. faecium can be achieved through several different mechanisms, including the presence of acquired ARGs like vat (D), vat (E), vgb (A), vgb (B), and eat (A), as well as the presence of the intrinsic gene, msr (C) (Boodaghi Malidareh et al, 2022 ; Miller et al, 2014 ). Almost all sequenced E. faecium strains in this study, except strain E5‐100, carried the msr (C) gene.…”

Section: Discussionmentioning

confidence: 99%

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Multidrug‐resistant Enterococcus faecium strains enter the Norwegian marine environment through treated sewage

Radisic,

Grevskott,

Junghardt

et al. 2024

MicrobiologyOpen

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This study aimed to understand the antibiotic resistance prevalence among Enterococcus spp. from raw and treated sewage in Bergen city, Norway. In total, 517 Enterococcus spp. isolates were obtained from raw and treated sewage from five sewage treatment plants (STPs) over three sampling occasions, with Enterococcus faecium as the most prevalent (n = 492) species. E. faecium strains (n = 307) obtained from the influent samples, showed the highest resistance against quinupristin/dalfopristin (67.8%). We observed reduced susceptibility to erythromycin (30.6%) and tetracycline (6.2%) in these strains. E. faecium strains (n = 185) obtained from the effluent samples showed highest resistance against quinupristin/dalfopristin (68.1%) and reduced susceptibility to erythromycin (24.9%) and tetracycline (8.6%). We did not detect resistance against last‐resort antibiotics, such as linezolid, vancomycin, and tigecycline in any of the strains. Multidrug‐resistant (MDR) E. faecium strains were detected in both influent (2.3%) and effluent (2.2%) samples. Whole genome sequencing of the Enterococcus spp. strains (n = 25) showed the presence of several antibiotic resistance genes, conferring resistance against aminoglycosides, tetracyclines, and macrolides, as well as several virulence genes and plasmid replicons. Two sequenced MDR strains from the effluents belonged to the hospital‐associated clonal complex 17 and carried multiple virulence genes. Our study demonstrates that clinically relevant MDR Enterococcus spp. strains are entering the marine environment through treated sewage.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Multidrug‐resistant Enterococcus faecium strains enter the Norwegian marine environment through treated sewage

Radisic,

Grevskott,

Junghardt

et al. 2024

MicrobiologyOpen

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“…E. faecalis shows a negative correlation with these antibiotics (p < 0.001), differing from that of E. faecium. This resistance pattern of E. faecalis is likely due to the lsa gene, which confers innate resistance to quinupristin/dalfopristin [5].…”

Section: Discussionmentioning

confidence: 99%

“…Enterococci inherently resist many antibiotics, including cephalosporins, aminoglycosides, trimethoprim-sulfamethoxazole, lincosamides, and quinolones. For instance, this intrinsic resistance to quinupristin/dalfopristin in E. faecalis is attributed to the lsa gene [5,6]. Furthermore, enterococci rapidly acquire resistance to antibiotics through mutations (various levels of resistance to aminoglycosides) or genetic transfers through plasmids, transposons, or integrons (resistance to glycopeptides, mainly vancomycin) [7].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Antibiotic Resistance and Biofilm Characteristics of Two Major Enterococcus Species from Poultry Slaughterhouses in South Korea

Son,

Jin,

Cho

et al. 2024

Veterinary Sciences

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The spread of antibiotic-resistant Enterococcus in the poultry industry poses significant public health challenges due to multidrug resistance and biofilm formation. We investigated the antibiotic resistance profiles and biofilm characteristics of E. faecalis and E. faecium isolates from chicken meat in poultry slaughterhouses in South Korea. Ninety-six isolates (forty-eight each of E. faecalis and E. faecium) were collected between March and September 2022. Both species were analyzed using MALDI-TOF, PCR, antibiotic susceptibility testing, and biofilm assays. A high level of multidrug resistance was observed in E. faecalis (95.8%) and E. faecium (93.8%), with E. faecium exhibiting a broader range of resistance, particularly to linezolid (52.1%) and rifampicin (47.9%). All E. faecalis isolates formed biofilm in vitro, showing stronger biofilm formation than E. faecium with a significant difference (p < 0.001) in biofilm strength. Specific genes (cob, ccf, and sprE) were found to be correlated with biofilm strength. In E. faecium isolates, biofilm strength was correlated with resistance to linezolid and rifampicin, while a general correlation between antibiotic resistance and biofilm strength was not established. Through analysis, correlations were noted between antibiotics within the same class, while no general trends were evident in other analyzed factors. This study highlights the public health risks posed by multidrug-resistant enterococci collected from poultry slaughterhouses, emphasizing the complexity of the biofilm-resistance relationship and the need for enhanced control measures.

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Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method

Wang,

Zhang,

Huo

et al. 2023

Arch Microbiol

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Dissemination of Quinupristin-Dalfopristin and Linezolid resistance genes among hospital environmental and healthy volunteer fecal isolates of Enterococcus faecalis and Enterococcus faecium

Cited by 4 publications

References 46 publications

Multidrug‐resistant Enterococcus faecium strains enter the Norwegian marine environment through treated sewage

Multidrug‐resistant Enterococcus faecium strains enter the Norwegian marine environment through treated sewage

Comparative Analysis of Antibiotic Resistance and Biofilm Characteristics of Two Major Enterococcus Species from Poultry Slaughterhouses in South Korea

Rapid and specific detection of Enterococcus faecium with an isothermal amplification and lateral flow strip combined method

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