2022
DOI: 10.1021/acs.langmuir.1c03096
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Dissimilar Deformation of Fluid- and Gel-Phase Liposomes upon Multivalent Interaction with Cell Membrane Mimics Revealed Using Dual-Wavelength Surface Plasmon Resonance

Abstract: The mechanical properties of biological nanoparticles play a crucial role in their interaction with the cellular membrane, in particular for cellular uptake. This has significant implications for the design of pharmaceutical carrier particles. In this context, liposomes have become increasingly popular, among other reasons due to their customizability and easily varied physicochemical properties. With currently available methods, it is, however, not trivial to characterize the mechanical properties of nanoscop… Show more

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Cited by 11 publications
(12 citation statements)
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“…For 200 nm LUVs, a drastic decrease in particle diameter calculated from SPR measurements is evident when the jamming limit is approached. An explanation for this behavior could be the deformation of vesicles [6,30], which would not be as prominent phenomena for EVs that are more rigid compared to LUVs. Both NTA and DLS measurements show that the peak of the size distribution for 200 nm LUVs is around a diameter of ca.…”
Section: Determination Of Size Of Luvs and Rbc Evsmentioning
confidence: 99%
“…For 200 nm LUVs, a drastic decrease in particle diameter calculated from SPR measurements is evident when the jamming limit is approached. An explanation for this behavior could be the deformation of vesicles [6,30], which would not be as prominent phenomena for EVs that are more rigid compared to LUVs. Both NTA and DLS measurements show that the peak of the size distribution for 200 nm LUVs is around a diameter of ca.…”
Section: Determination Of Size Of Luvs and Rbc Evsmentioning
confidence: 99%
“…NPs are often engineered with two classes of surface modifications that affect this interaction: targeting moieties to promote NP interactions with specific receptors and cell types, and shielding polymers like polyethylene glycol (PEG) to increase in vivo circulation time and reduce the rate of biofouling in biological systems . While many intrinsic properties of NPs affect ligand-receptor binding, such as stiffness, ligand mobility, and surface roughness, an important, often less explored feature is the spatial presentation of conjugated ligands on the NP surface. Ligand density is an important parameter that controls bond multivalency when targeting a NP to a cellular receptor. This density affects the overall binding strength of the NP to the target cell, or NP avidity, and ultimately contributes to the physiological fate of the NP and overall efficacy of drug delivery.…”
Section: Introductionmentioning
confidence: 99%
“…Since planar-supported lipid bilayers (SLBs) and nanoscopic lipid vesicles can be readily prepared and tuned by varying preparation conditions, such nanoarchitectures have been extensively utilized in most of the relevant biophysical studies as simple models of the cell membrane and LNPs/extracellular vesicles, respectively. , Early work focused on developing a measurement platform to monitor the attachment of biotin-modified lipid vesicles onto a streptavidin-coated SLB surface and resulting vesicle shape deformation in multivalent ligand–receptor interaction contexts . More recently, this measurement approach has gained scientific interest to study various factors that influence the membrane properties of LNPs such as nanoparticle size, , lipid composition, , and cholesterol fraction, which indicates the potential to further assess the effect of environmental conditions, including salt and solution pH …”
Section: Introductionmentioning
confidence: 99%