Dissimilarity ofccrABgene sequences between methicillin-resistantStaphylococcus epidermidisand methicillin-resistantStaphylococcus aureusamong bovine isolates in Korea

Abstract

The sequences of the ccrAB genes from bovine-, canine- and chicken-originating methicillin-resistant Staphylococcus (S.) epidermidis (MRSE) and bovine methicillin-resistant Staphylococcus (S.) aureus (MRSA) were compared to investigate the frequency of intra-species horizontal transfer of the staphylococcal cassette chromosome mec (SCCmec) complex. Nineteen MRSE strains were isolated from bovine milk, chickens, and dogs, and their genetic characteristics were investigated by multilocus sequence typing and SCCmec typing. Among the animal MRSE strains, the most frequent SCCmec type was type IV, which consisted of the type B mec complex and ccrAB type 2. The ccrA2 and ccrB2 genes were sequenced from the bovine, chicken and canine MRSE strains and compared with those of the bovine MRSA strains. The sequences generally clustered as MRSA and MRSE groups, regardless of the animal source. Additionally, no bovine MRSE sequence was associated with the bovine MRSA groups. Although most of the bovine MRSE and MRSA isolates possessed SCCmec type IV sequences, our results suggest that the intra-species gene transfer of the SCCmec complex between bovine S. aureus and bovine S. epidermidis strains is not a frequent event.

Antimicrobial resistance and population structure of Staphylococcus epidermidis recovered from pig farms in Belgium

“…SCCmec type IV, carried by most MRSE isolates identified in the present study, has been reported to be the most frequent type of MRSE in animals (Park et al, 2013) and humans (Miragaia et al, 2008;Barbier et al, 2010). The other SCCmec types (V and NT) have been described in MRSE isolates from infections of companion animals (Kern and Perreten, 2013), in ovine mastitis (Onni et al, 2011) and healthy cattle .…”

“…The predominance, widespread distribution and diversity of SCCmec in S. epidermidis suggest that this bacterial species might be a potential reservoir of this cassette for S. aureus or other staphylococci. However, recent research has suggested that intra-species transfer of the SCCmec complex between S. aureus and S. epidermidis is not a frequent event, at least in cattle (Park et al, 2013). The role of S. epidermidis in pigs as a SCCmec reservoir for S. aureus should be further investigated.…”

Clonally Diverse Methicillin and Multidrug Resistant Coagulase Negative Staphylococci Are Ubiquitous and Pose Transfer Ability Between Pets and Their Owners

“…Lack of robust concordance was observed between results obtained by both schemes, with guidelines from Kondo et al (2007) showing a remarkable high diversity index (SID 0.89 versus 0.71), and reflecting the high intergenic diversity within MRCoNS cassettes. SCC mec IV was the predominant typeable cassette for both owner and pets, and despite, additional cassettes have been sporadically detected, it is also the most prevalent cassette among humans and companion animals (Ruppe et al, 2009; Barbier et al, 2010; Lebeaux et al, 2012; Aslantas et al, 2013; Kern and Perreten, 2013; Park et al, 2013; Weiss et al, 2013; Becker et al, 2014; Abadi et al, 2015; McManus et al, 2015; Couto et al, 2016).…”

Livestock-Associated MRSA and Its Current Evolution

“…As shown [158], there is much more variation in the sequences, and ideally the SCCmec types should be subdivided based on differences in sequences. This task has become feasible with new-generation sequencing; therefore, it no longer will be a great challenge to develop a more detailed subtyping scheme of SCCmec types to enable researchers to follow their movements, plasticity, and epidemiology.…”