In agriculture, fungi can cause serious damage, resulting in critical losses of yield, quality and profit. Fungicides help in reducing the damage caused by fungus, reduce the yield loss and play a major role in quality improvement. The present investigation was carried out at Tamil Nadu Agricultural University to evaluate the influence of trifloxystrobin 50% + tebuconazole 25% (Nativo 75WG) on the yield and quality improvement on Bunny hybrid Bt cotton. Nativo 75WG was applied on the leaves of cotton plants at 40-60 (DAS) and 60-80 (DAS) at the concentration of 250, 300, 350 g/ha and Carbendazim @ 500 g/ha. The observations recorded were related to yield and quality attributes in all treatments. The application of Nativo @ 300 g/ha showed a significant increase in boll weight (4.86 g), lint yield per boll (3.86 g boll-1) and lint per plant (138.48 g plant -1) than other treatments. With respect to seed cotton yield and harvest index (0.37 %), the Nativo @ 300 g/ha registered a higher yield (20.2 %) and HI than control under the irrigated situation. Foliar application of treatments during the flowering stage (40-60 DAS) and boll formation stages (60-80 DAS) had increased the quality parameters such as fiber length (2.5% staple length, 50 % staple length) and fiber strength. Further, the foliar spray of Nativo @ 300 g/ha applied to bunny hybrid Bt cotton had resulted in a higher yield (2920.15 kg ha-1) due to an increase in leaf area index, greenness of leaf and higher dry matter production of the plant.