Dissipative interactions in cell–matrix adhesion

Müller, Christina; Müller, Andreas; Pompe, Tilo

doi:10.1039/c3sm50803j

Cited by 26 publications

(23 citation statements)

References 74 publications

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“…In addition to elasticity, most hydrogels, especially biopolymer‐based hydrogels, show both elastic and viscous properties . These hydrogels are viscoelastic and often exhibit stress relaxation (e.g., a decrease in stress in response to constant applied strain) or creep (e.g., an increase in strain in response to constantly applied stress) behaviors.…”

Section: Mechanical Properties Of Hydrogels For 3d Stem Cell Culture mentioning

confidence: 99%

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

et al. 2018

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Stem cells hold great promise for widespread biomedical applications, for which stem cell fate needs to be well tailored. Besides biochemical cues, accumulating evidence has demonstrated that spatiotemporal biophysical cues (especially mechanical cues) imposed by cell microenvironments also critically impact on the stem cell fate. As such, various biomaterials, especially hydrogels due to their tunable physicochemical properties and advanced fabrication approaches, are developed to spatiotemporally manipulate biophysical cues in vitro so as to recapitulate the 3D mechanical microenvironment where stem cells reside in vivo. Here, the main mechanical cues that stem cells experience in their native microenvironment are summarized. Then, recent advances in the design of hydrogel materials with spatiotemporally tunable mechanical properties for engineering 3D the spatiotemporal mechanical microenvironment of stem cells are highlighted. These in vitro engineered spatiotemporal mechanical microenvironments are crucial for guiding stem cell fate and their potential biomedical applications are subsequently discussed. Finally, the challenges and future perspectives are presented.

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Section: Mechanical Properties Of Hydrogels For 3d Stem Cell Culture mentioning

confidence: 99%

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

et al. 2018

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“…This signaling process is facilitated by a complex interplay between transmembrane and intracellular proteins of multicomponent adhesion sites, which link the cytoskeleton with the extracellular matrix (ECM). 10,16 The remodeling of the ECM accompanying cell adhesion and migration implies a continuous molecular friction of adhesion ligands and thus energy dissipation. 1, 2 Viscoelastic properties of the ECM influence cell fate beyond simple adhesive forces.…”

Section: Introductionmentioning

confidence: 99%

Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Müller

Pompe

2016

Soft Matter

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Cell adhesion is regulated by the mechanical characteristics of the cell environment. The influences of different parameters of the adhesive substrates are convoluted in the cell response leading to questions on the underlying mechanisms, like biochemical signaling on the level of adhesion molecules, or viscoelastic properties of substrates and cell. By a time-resolved analysis of traction force generation during early cell adhesion, we wanted to elucidate the contributions of substrate mechanics to the adhesion process, in particular the impact of substrate elasticity and the molecular friction of adhesion ligands on the substrate surface. Both parameters were independently adjusted by (i) an elastic polyacrylamide hydrogel of variable crosslinking degree and (ii) a thin polymer coating of the hydrogel surface controlling the affinity (and the correlated substrate-ligand friction) of the adhesion ligand fibronectin. Our analysis showed two sequential regimes of considerable force generation, whose occurrence was found to be independent of substrate properties. The first regime is characterized by spreading of the cell and a succeeding force increase. After spreading cells enter the second regime with saturated forces. Substrate elasticity and viscosity, namely hydrogel elasticity and ligand affinity, were both found to affect the kinetics and absolute levels of traction force quantities. A faster increase and a higher saturation level of traction forces were observed for a higher substrate stiffness and a higher ligand affinity. The results complement recent modeling approaches on the evolution of forces in cell spreading and contribute to a better understanding of the dynamics of cell adhesion on viscoelastic substrates.

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“…On 2D substrates, lateral forces and friction between cells and ECM interface were recently interpreted as being coresponsible for the signaling pathways during mechanotransduction, as cells are capable of reorganizing ligand presentation to an extent that is directly related to the dissipative frictional forces which originate at the ECM surface . Hence, interfaces featuring precisely tunable friction and allowing controlled tethering of cues could in principle actively control the mechanism of cell adhesion and, consequently, mechanotransduction.…”

Section: Introductionmentioning

confidence: 99%

Stem‐Cell Clinging by a Thread: AFM Measure of Polymer‐Brush Lateral Deformation

Gunnewiek

Ramakrishna

Luca

et al. 2015

Adv Materials Inter

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If the binding strength of adhesive cues to the extracellular matrix (ECM) and the mechanisms involved in cell adhesion are synergistically correlated via a “mechanical” feedback, engineering of cue presentation at the ECM by designer macromolecules can enable control over cell–matrix interaction. Here, polymer brushes supporting fibronectin (FN) and presenting different grafted‐chain length to modulate cell interaction at ECM cell‐binding sites are exploited. Application of friction force microscopy allows us to estimate the lateral deformability and friction of oligoethylene glycol‐containing brushes. These parameters are demonstrated to regulate the adhesion of human mesenchymal stem cells (hMSCs), which adopt their morphology and form focal adhesions (FAs) responding to FN brush‐tether characteristics. Across a brush‐thickness gradient presenting uniform FN exposure, thin brushes stimulate cell spreading and the development of FAs. Conversely, thick and more laterally deformable polymer grafts induce a decrease in cell spreading and FA formation. A correlation between frictional forces experienced at the (macro)molecular scale and the behavior of stem cells has been found. This interaction can be clarified by exploring novel aspects of FFM, which demonstrates a powerful tool to dynamically probe the ECM environment and indirectly suggest a way to structure ECM in order to trigger specific cell responses.

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Dissipative interactions in cell–matrix adhesion

Cited by 26 publications

References 74 publications

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Stem‐Cell Clinging by a Thread: AFM Measure of Polymer‐Brush Lateral Deformation

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