Dissociating effects of cocaine and d-amphetamine on dopamine and serotonin in the perirhinal, entorhinal, and prefrontal cortex of freely moving rats

Pum, Martin; Carey, Robert J.; Huston, Joseph P.; Müller, Christian P.

doi:10.1007/s00213-007-0791-2

Cited by 81 publications

(65 citation statements)

References 91 publications

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“…Samples were collected every 20 min. Three samples were taken as baseline and the neurotransmitters DA, 5-HT and NA were quantified by HPLC-EC as described previously Pum et al, 2007). An injection of alcohol was then administered i.p.…”

Section: In Vivo Microdialysismentioning

confidence: 99%

αCaMKII Autophosphorylation Controls the Establishment of Alcohol Drinking Behavior

Easton

Lucchesi

Lourdusamy

et al. 2013

Neuropsychopharmacol

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The a-Ca 2 þ /calmodulin-dependent protein kinase II (aCaMKII) is a crucial enzyme controlling plasticity in the brain. The autophosphorylation of aCaMKII works as a 'molecular memory' for a transient calcium activation, thereby accelerating learning. We investigated the role of aCaMKII autophosphorylation in the establishment of alcohol drinking as an addiction-related behavior in mice. We found that alcohol drinking was initially diminished in aCaMKII autophosphorylation-deficient aCaMKII T286A mice, but could be established at wild-type level after repeated withdrawals. The locomotor activating effects of a low-dose alcohol (2 g/kg) were absent in aCaMKII T286A mice, whereas the sedating effects of high-dose (3.5 g/kg) were preserved after acute and subchronic administration. The in vivo microdialysis revealed that aCaMKII T286A mice showed no dopamine (DA) response in the nucleus accumbens to acute or subchronic alcohol administration, but enhanced serotonin (5-HT) responses in the prefrontal cortex. The attenuated DA response in aCaMKII T286A mice was in line with altered c-Fos activation in the ventral tegmental area after acute and subchronic alcohol administration. In order to compare findings in mice with the human condition, we tested 23 single-nucleotide polymorphisms (SNPs) in the CAMK2A gene for their association with alcohol dependence in a population of 1333 male patients with severe alcohol dependence and 939 controls. We found seven significant associations between CAMK2A SNPs and alcohol dependence, one of which in an autophosphorylation-related area of the gene. Together, our data suggest aCaMKII autophosphorylation as a facilitating mechanism in the establishment of alcohol drinking behavior with changing the DA-5-HT balance as a putative mechanism.

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Section: In Vivo Microdialysismentioning

confidence: 99%

αCaMKII Autophosphorylation Controls the Establishment of Alcohol Drinking Behavior

Easton

Lucchesi

Lourdusamy

et al. 2013

Neuropsychopharmacol

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“…Amphetamine (0.5 mg/kg) was administered 15 min prior to CR testing to ensure testing commenced during the peak of amphetamine effects. This interval was chosen based on previous behavioral data from our lab (Tenn et al 2003(Tenn et al , 2005 and previous neurochemical findings (Di Chiara and Imperato 1988;McKittrick and Abercrombie 2007;Pum et al 2007). Psychostimulant selfadministration during adolescence may shift the peak of amphetamine effects; thus, injecting amphetamine immediately prior to the session may have revealed group differences.…”

Section: Discussionmentioning

confidence: 99%

The effects of adolescent methylphenidate self-administration on responding for a conditioned reward, amphetamine-induced locomotor activity, and neuronal activation

2009

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“…Animals were placed into a 20 × 20-cm open field, and experiments were initiated after stable levels of DA transmitter had been obtained. As previously detailed (43), DA levels were determined in dialysate samples taken at 20-min intervals over a period of 3 h by use of HPLC with electrochemical detection. Because no zero-net flux methodology could be used, basal transmitter levels in mutants can only be interpreted in relation to WT levels.…”

Section: Methodsmentioning

confidence: 99%

RASGRF2 regulates alcohol-induced reinforcement by influencing mesolimbic dopamine neuron activity and dopamine release

Stacey

Bilbao

Maroteaux

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

102

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The firing of mesolimbic dopamine neurons is important for druginduced reinforcement, although underlying genetic factors remain poorly understood. In a recent genome-wide association metaanalysis of alcohol intake, we identified a suggestive association of SNP rs26907 in the ras-specific guanine-nucleotide releasing factor 2 (RASGRF2) gene, encoding a protein that mediates Ca 2+ -dependent activation of the ERK pathway. We performed functional characterization of this gene in relation to alcohol-related phenotypes and mesolimbic dopamine function in both mice and adolescent humans. Ethanol intake and preference were decreased in Rasgrf2 −/− mice relative to WT controls. Accordingly, ethanol-induced dopamine release in the ventral striatum was blunted in Rasgrf2 −/− mice. Recording of dopamine neurons in the ventral tegmental area revealed reduced excitability in the absence of Ras-GRF2, likely because of lack of inhibition of the I A potassium current by ERK. This deficit provided an explanation for the altered dopamine release, presumably linked to impaired activation of dopamine neurons firing. Functional neuroimaging analysis of a monetary incentive-delay task in 663 adolescent boys revealed significant association of ventral striatal activity during reward anticipation with a RASGRF2 haplotype containing rs26907, the SNP associated with alcohol intake in our previous metaanalysis. This finding suggests a link between the RASGRF2 haplotype and reward sensitivity, a known risk factor for alcohol and drug addiction. Indeed, follow-up of these same boys at age 16 y revealed an association between this haplotype and number of drinking episodes. Together, these combined animal and human data indicate a role for RASGRF2 in the regulation of mesolimbic dopamine neuron activity, reward response, and alcohol use and abuse.I A current | neuroimaging genetic reward-anticipation preference T he reinforcing properties of addictive drugs are dependent on the activity of mesolimbic dopamine (DA) neurons in the ventral tegmental area (VTA) and their projections to the ventral striatum (VS) and prefrontal cortex (PFC) (1). Microdialysis studies using rodent models have shown that acute administration of addictive drugs, including alcohol, results in elevated DA levels in the VS (2). This effect results from local inhibition of DA reuptake, stimulation of its release, or an increase in firing rate of DA neurons in the VTA (3-5). PET studies have shown a similar effect in the VS of humans as evidenced by decreased competitive binding of a DA receptor antagonist [ 11 C] raclopride (6, 7). Illustrating the importance of DA signaling in the regulation of alcohol-induced reinforcement, rats are known to self-administer ethanol in the nucleus accumbens (8) and posterior VTA (9). These studies and other animal studies suggest that midbrain DA neurons are involved in the acquisition of primary alcohol reinforcement (review in ref. 1).Although the neurobiological and molecular mechanisms controlling DA neuron activity by different ...

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Dissociating effects of cocaine and d-amphetamine on dopamine and serotonin in the perirhinal, entorhinal, and prefrontal cortex of freely moving rats

Abstract: We conclude that cocaine and d-amphetamine increase DA and 5-HT levels in PRC and EC largely to the same extent as in the PFC.

Cited by 81 publications

References 91 publications

αCaMKII Autophosphorylation Controls the Establishment of Alcohol Drinking Behavior

αCaMKII Autophosphorylation Controls the Establishment of Alcohol Drinking Behavior

The effects of adolescent methylphenidate self-administration on responding for a conditioned reward, amphetamine-induced locomotor activity, and neuronal activation

RASGRF2 regulates alcohol-induced reinforcement by influencing mesolimbic dopamine neuron activity and dopamine release

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