We report on the observation of half-quantum vortices (HQVs) in the easy-plane polar phase of an antiferromagnetic spinor Bose-Einstein condensate. Using in situ magnetization-sensitive imaging, we observe that pairs of HQVs with opposite core magnetization are generated when singly charged quantum vortices are injected into the condensate. The dynamics of HQV pair formation is characterized by measuring the temporal evolutions of the pair separation distance and the core magnetization, which reveals the short-range nature of the repulsive interactions between the HQVs. We find that spin fluctuations arising from thermal population of axial magnon excitations do not significantly affect the HQV pair formation dynamics. Our results demonstrate the instability of a singly charged vortex in the antiferromagnetic spinor condensate. In a scalar superfluid, the supercurrent circulation around quantum vortices is quantized in units of h/m due to U (1) gauge symmetry [1], where h is the Planck constant and m is particle mass. However, when a superfluid possesses an internal spin degree of freedom, there is an intriguing possibility for the superfluid to host quantum vortices of a fractional circulation of h/m. The superfluid phase interwinds with the spin orientation and a new relation is imposed on the supercurrent circulation in connection with spin texture [2]. Fractional quantum vortices are of particular interest in two-dimensional (2D) superfluidity. In the absence of long-range order in two dimensions [3], the superfluid phase transition in 2D is associated with vortex-antivortex pairing as described by the Berezinskii-Kosterlitz-Thouless (BKT) theory [4,5]. Hence fractional quantum vortices, introduced as new point defects, represent an interesting opportunity to explore for exotic superfluid phases, possibly beyond the BKT physics.Quantum vortices having h/2m circulation, so-called half-quantum vortices (HQVs) have been experimentally observed in spinor superfluid systems such as excitonpolariton condensates [6][7][8] and triplet superconductors [9]. In previous cold atom experiments, HQV states were created with an optical method in two-component Bose-Einstein condensates (BECs) [10], where the two components are not symmetric in terms of interactions. Recently, a spin-1 BEC with antiferromagnetic interactions has been considered with great interest because HQVs are topologically allowed in the polar phase of the system [11][12][13]. Theoretical studies predicted an anomalous superfluid density jump at the phase transition in two dimensions [14][15][16] as well as a new superfluid state that has completely broken spin ordering [17,18].In this Letter, we report on the observation of HQVs in the easy-plane polar phase of an antiferromagnetic spinor BEC of 23 Na atoms. Using magnetization-sensitive imaging, we observe that pairs of HQVs with opposite core magnetization are generated when singly charged quantum vortices are injected into the condensate. The temporal evolutions of the pair separation distance and t...