Dissociation in a marine pseudomonad

Abstract

Eight morphological variants, the product of colonial dissociation, were isolated from cultures of the marine pseudomonad B-16. Features which distinguished the variants were smooth versus rough colony type, the presence or absence of color, and differences in colony diameter. The variants differed in their capacity to form stable protoplasts and to grow at suboptimal Na+ concentrations in defined medium. All, however, required Na+ for growth. The ability of the organism to accumulate α-aminoisobutyric acid (AIB) and the requirement for Na+ for this process was not affected by dissociation.

Effect of strain variation and growth phase of culture on dry weight and hexosamine content of cell wall layers of a marine pseudomonad

“…Variant 7 was isolated originally from a translucent sector of a colony of variant 3 (7). Variant 3 is considered to be the normal form of the organism and is the one which has been used most freauentlv in this laboratory for physiological studies.…”

“…Variant 3 is considered to be the normal form of the organism and is the one which has been used most freauentlv in this laboratory for physiological studies. The p;oced;res used to maintain the cultures and the composition of the complex medium which was used to grow the cells have been described (7). All cultures were grown at 25°C in Erlenmeyer flasks on a rotary shaker.…”

“…Eight morphological variants, the product of colonial dissociation, have been isolated from cultures of the marine pseudomonad B-16 (7). Features which distinguish the variants are smooth versus rough colony type, the presence or absence of opacity and color in colonies produced, and differences in colony diameter.…”

Effects of wash treatments on the ultrastructure and lysozyme penetrability of the outer membrane of various marine and two terrestrial gram-negative bacteria

“…For day to day use cultures of A. haloplanktis were maintained as described previously (Gow et al 1973). Photobacterium phosphoreum was maintained at 4OC and all other cultures at 19 to 22OC by monthly transfer on slants of Difco marine agar 2216.…”

Kinetics of Na+-dependent K+ ion transport in a marine pseudomonad

“…Thus, deplasmolysis required the presence of both Na+ and K+ in the suspending medium. This dependence of K+ accumulation upon the presence of Na+ was attributed to the ability of Na+ to cause the membrane to retain the K+ accumulated by the cells (20 (12), and was recently reclassified as Alteromonas haloplanktis (17). The organism has been deposited in the National Collection of Marine Bacteria (NCMB 19) and in the American Type Culture Collection (ATCC 19855).…”