Dissociation of actomyosin complex by monovalent fragments of polyclonal antibodies to myosin

Mazander, K.D.; Gröschel‐Stewart, Ute

doi:10.1016/0014-5793(85)80317-3

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1997

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“…We have previously shown that polyclonal antibodies elicited by immunization with native chicken fast-twitch myosin inhibited ATPase activity and actin-binding (8,9). We then noticed that antibodies to SDS-denatured myosin heavy chain, shown to react with myosin in Western blotting, enzyme immunoassay and immunofluorescence, did not influence its enzymatic activity.…”

Section: Introductionmentioning

confidence: 99%

Heat treated myosin does not bind ATPase ‐ inhibiting antibodies

1997

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Polyclonal antibodies to native chicken pectoral fast-twitch myosin are directed to all subfragments of the molecule ($1, $2 and LMM), as seen in the ELISA and Western blotting techniques. The antibodies inhibit the Ca2+-activated myosin ATPase. Absorption of the antibodies with native myosin abolishes these reactions. Heat treatment of myosin for 2h at 40~ will inactivate myosin ATPase and alter its antibody binding pattern: the binding of antibodies to the rod fractions is reduced, that to the globular head ($1) completely abolished. Thus, these antibodies are useful as sensitive probes for the structural integrity of the myosin head.

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Section: Introductionmentioning

confidence: 99%