Dissociation of macromolecular ribonucleoprotein of yeast

Chao, Fu-Chuan

doi:10.1016/0003-9861(57)90130-3

Cited by 153 publications

(42 citation statements)

References 2 publications

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“…(Chao & Schachman, 1956); a similar value was also reported by Bowen, Dagley, Sykes & Wild (1961). In solutions poor in Mg and Cay the 80 S ribosome dissociated into 60 and 40 S particles (Chao, 1957); papain action led to a similar breakdown (Morgan, Greenspan & Cunningham, 1963 …”

Section: Introductionsupporting

confidence: 67%

Sedimentation Coefficients of Yeast Ribosomes

Ley

1964

Journal of General Microbiology

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SUNZMARYThe corrected sedimentation coefficients, expressed as (S20, a)O, of ribosomes from nine different yeasts, representing a wide taxonomic range, were found to be 51.5 f 2.4, 68.6 f 3.3 and 86.7 & 2.9 x see. Very small quantities of a faster (approximately 128 x sec.) and a slower particle were sometimes detected. Yeast ribosomes have thus sedimentation coefficients which are distinctly different from bacterial ribosomes.

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Section: Introductionsupporting

confidence: 67%

Sedimentation Coefficients of Yeast Ribosomes

Ley

1964

Journal of General Microbiology

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“…Magnesium, which is known to stabilize isolated ribosomes (see review by McQuillen, 1962), decreased both rates, whereas EDTA, which accelerates ribosomal breakdown (Chao, 1957; Wade, 1961), increased both rates. Magnesium, manganese, cobalt and calcium, which afforded good or some protection to A. aerogenes during heating, all strongly inhibited the auto-degradation at pH 6.5 of purified ribosomes isolated from Escherichia coli by Mr H. E. Wade. In most cases the shape of the death and leakage curves provided evidence that RNA breakdown preceded bacterial death a t M", there being an initial period of zero or small death-rate during which RNA degradation occurred a t the maximum rate.…”

Section: Discussionmentioning

confidence: 99%

Effects of Thermal Stress on Viability and Ribonucleic Acid of Aerobacter aerogenes in Aqueous Suspension

Strange¹,

Shon²

1964

Journal of General Microbiology

105

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SUMMARYThe death-rate of washed Aerobucter aerogernes in aqueous suspension at 47" depended on the nature of the growth medium, the composition of the liquid used to wash and resuspend the bacteria, the bacterial growth phase, the bacterial concentration in heated suspensions, the pH value, the oxygen tension and the composition of the diluent in which bacteria were heated. The relative resistance of bacteria in different growth phases differed according to the growth medium and the washing fluid; stationary phase bacteria were not more resistant than exponential phase organisms under all conditions. Starvation increased the thermal resistance of exponential and stationary phase bacteria. High bacterial concentration favoured survival a t 47" under most conditions; cell-free filtrate from a heated dense suspension (1010 bacteria/ml.) protected a sparser population of fresh bacteria ( 107-10g/ml.) heated in it. Protective material in filtrate was heat-stable ( 100°/15 min.) and diffused through cellophan. The optimum pH value for survival at 47" was near pH 6-5. Aerobic conditions favoured survival in distilled water but not in salt solutions or phosphate saline (pH 6-5). The effects of various concentrations of NaCl and KC1 on the survival of bacteria at 47' under aerobic conditions were different, K+ concentrations above 0.1 M being more lethal than equivalent concentrations of Na+; the lethal effect of heating in mixtures of these salts (total M > 0.1) increased with K+ concentration. Growth medium, Mg2+ (0-01-5 mM) and, to a lesser extent, Mn*+ (0.5 mM) or Co2+ ( 5 mM) decreased the death-rate, whereas ethylenediamine tetraacetic acid (mM), or various sugars, increased it. Mga+ but not M n 2 + reversed the lethal effect of sugars.Generally, conditions which accelerated the death-rate of Aerobucter wogem?s at 47" also increased the rate of degradation of endogenous RNA. This was accompanied by an increase in the ultraviolet absorption of cold acid-extracts of bacteria and of the suspending fluid. Bacterial protein was degraded to a smaller extent. Depletion of RNA is probably not the primary cause of death a t 47" but the effect on bacterial metabolism of a rapid increase in endogenous pool constituents resulting from RNA degradation may contribute to the lethal effect.

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“…For example, the in vitro association of the small and large ribosomal subunits to form intact ribosomes depends strongly on Mg 2+ ion concentration (Chao and Schachman 1956;Chao 1957;Tissieres et al 1959). Further, growth of Escherichia coli cells under conditions of Mg 2+ starvation result in ribosome depletion (McCarthy 1962), suggesting that Mg 2+ is essential for the assembly and structural integrity of ribosomes.…”

Section: Introductionmentioning

confidence: 99%

The contribution of metal ions to the structural stability of the large ribosomal subunit

Klein¹,

Moore²,

Steitz³

2004

RNA

297

378

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Both monovalent cations and magnesium ions are well known to be essential for the folding and stability of large RNA molecules that form complex and compact structures. In the atomic structure of the large ribosomal subunit from Haloarcula marismortui, we have identified 116 magnesium ions and 88 monovalent cations bound principally to rRNA. Although the rRNA structures to which these metal ions bind are highly idiosyncratic, a few common principles have emerged from the identities of the specific functional groups that coordinate them. The nonbridging oxygen of a phosphate group is the most common inner shell ligand of Mg

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Dissociation of macromolecular ribonucleoprotein of yeast

Cited by 153 publications

References 2 publications

Sedimentation Coefficients of Yeast Ribosomes

Sedimentation Coefficients of Yeast Ribosomes

Effects of Thermal Stress on Viability and Ribonucleic Acid of Aerobacter aerogenes in Aqueous Suspension

The contribution of metal ions to the structural stability of the large ribosomal subunit

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